ID   OEP16_PEA               Reviewed;         146 AA.
AC   Q41050;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Outer envelope pore protein 16, chloroplastic;
DE   AltName: Full=Chloroplastic outer envelope pore protein of 16 kDa;
GN   Name=OEP16;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND BETA STRANDS.
RC   STRAIN=cv. Golf; TISSUE=Leaf;
RX   PubMed=9256512; DOI=10.1073/pnas.94.17.9504;
RA   Pohlmeyer K., Soll J., Steinkamp T., Hinnah S., Wagner R.;
RT   "Isolation and characterization of an amino acid-selective channel protein
RT   present in the chloroplastic outer envelope membrane.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:9504-9509(1997).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=10998242; DOI=10.1021/bi001034m;
RA   Linke D., Frank J., Holzwarth J.F., Soll J., Boettcher C., Fromme P.;
RT   "In vitro reconstitution and biophysical characterization of OEP16, an
RT   outer envelope pore protein of pea chloroplasts.";
RL   Biochemistry 39:11050-11056(2000).
RN   [3]
RP   FUNCTION, TOPOLOGY, AND MUTAGENESIS OF CYS-71.
RX   PubMed=10766798; DOI=10.1074/jbc.275.16.11758;
RA   Steinkamp T., Hill K., Hinnah S.C., Wagner R., Roehl T., Pohlmeyer K.,
RA   Soll J.;
RT   "Identification of the pore-forming region of the outer chloroplast
RT   envelope protein OEP16.";
RL   J. Biol. Chem. 275:11758-11764(2000).
RN   [4]
RP   FUNCTION, TOPOLOGY, AND SUBUNIT.
RX   PubMed=21878393; DOI=10.1016/j.pep.2011.08.004;
RA   Ni D.Q., Zook J., Klewer D.A., Nieman R.A., Soll J., Fromme P.;
RT   "Isolation, folding and structural investigations of the amino acid
RT   transporter OEP16.";
RL   Protein Expr. Purif. 80:157-168(2011).
CC   -!- FUNCTION: Voltage-dependent high-conductance channel with a slight
CC       cation-selectivity; selective for amino acids but excludes
CC       triosephosphates or uncharged sugars. Non-essential amino acid-
CC       selective channel protein and translocation pore for
CC       NADPH:protochlorophyllide oxidoreductase A (PORA) and possibly PORB.
CC       {ECO:0000269|PubMed:10766798, ECO:0000269|PubMed:21878393,
CC       ECO:0000269|PubMed:9256512}.
CC   -!- SUBUNIT: Homodimer and oligomers in membrane.
CC       {ECO:0000269|PubMed:21878393, ECO:0000269|PubMed:9256512}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Plastid,
CC       etioplast membrane {ECO:0000250}; Multi-pass membrane protein
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. Plastid outer
CC       envelope porin OEP16 (TC 1.B.30) subfamily. {ECO:0000305}.
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DR   EMBL; Z73553; CAA97910.1; -; mRNA.
DR   PIR; T06471; T06471.
DR   AlphaFoldDB; Q41050; -.
DR   BMRB; Q41050; -.
DR   SMR; Q41050; -.
DR   TCDB; 1.B.30.1.1; the plastid outer envelope porin of 16 kda (oep16) family.
DR   EnsemblPlants; Psat5g284600.2; Psat5g284600.2.cds; Psat5g284600.
DR   Gramene; Psat5g284600.2; Psat5g284600.2.cds; Psat5g284600.
DR   GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034426; C:etioplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0045037; P:protein import into chloroplast stroma; IDA:UniProtKB.
DR   InterPro; IPR045238; Tim23-like.
DR   PANTHER; PTHR15371; PTHR15371; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Ion transport; Membrane; Plastid; Plastid outer membrane;
KW   Porin; Transmembrane; Transmembrane beta strand; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..146
FT                   /note="Outer envelope pore protein 16, chloroplastic"
FT                   /id="PRO_0000415700"
FT   TRANSMEM        75..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..73
FT                   /note="Contains 4 beta strands"
FT   MUTAGEN         71
FT                   /note="C->S: Loss of CuCl(2) sensitivity of the channel."
FT                   /evidence="ECO:0000269|PubMed:10766798"
SQ   SEQUENCE   146 AA;  15490 MW;  B72E24ABF6AAC2AB CRC64;
     MPRSSFSGSL SSPKLDVVID MGNPFLNLTV DGFLKIGAVA ATRSVAEDTF HIIRKGSISS
     NDFEKSLKKM CKEGAYWGAI AGVYVGMEYG VERIRGTRDW KNAMFGGAVT GALVSAASNN
     KKDKIAVDAI TGAAIATAAE FINYLT