OEP7_ARATH
ID OEP7_ARATH Reviewed; 64 AA.
AC Q9SVC4;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Outer envelope membrane protein 7 {ECO:0000303|PubMed:11595795};
DE Short=AtOEP7 {ECO:0000303|PubMed:11595795};
GN Name=OEP7 {ECO:0000303|PubMed:11595795};
GN OrderedLocusNames=At3g52420 {ECO:0000312|EMBL:AEE78944.1};
GN ORFNames=F22O6.200 {ECO:0000312|EMBL:CAB43440.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN, AND MUTAGENESIS OF
RP 10-ALA--LEU-22; ALA-15; LEU-19; ALA-23; LYS-29; ASP-33 AND LYS-34.
RC STRAIN=cv. Columbia;
RX PubMed=11595795; DOI=10.2307/3871501;
RA Lee Y.J., Kim D.H., Kim Y.-W., Hwang I.;
RT "Identification of a signal that distinguishes between the chloroplast
RT outer envelope membrane and the endomembrane system in vivo.";
RL Plant Cell 13:2175-2190(2001).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=17337631; DOI=10.1105/tpc.106.047407;
RA Duy D., Wanner G., Meda A.R., von Wiren N., Soll J., Philippar K.;
RT "PIC1, an ancient permease in Arabidopsis chloroplasts, mediates iron
RT transport.";
RL Plant Cell 19:986-1006(2007).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH AKR2A.
RC STRAIN=cv. Columbia;
RX PubMed=18193034; DOI=10.1038/ncb1683;
RA Bae W., Lee Y.J., Kim D.H., Lee J., Kim S., Sohn E.J., Hwang I.;
RT "AKR2A-mediated import of chloroplast outer membrane proteins is essential
RT for chloroplast biogenesis.";
RL Nat. Cell Biol. 10:220-227(2008).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=18715957; DOI=10.1104/pp.108.123075;
RA Oikawa K., Yamasato A., Kong S.-G., Kasahara M., Nakai M., Takahashi F.,
RA Ogura Y., Kagawa T., Wada M.;
RT "Chloroplast outer envelope protein CHUP1 is essential for chloroplast
RT anchorage to the plasma membrane and chloroplast movement.";
RL Plant Physiol. 148:829-842(2008).
RN [9]
RP AKR2A-BINDING SEQUENCE, AND REVIEW.
RX PubMed=21057222; DOI=10.4161/psb.5.11.13714;
RA Zhang H., Li X., Zhang Y., Kuppu S., Shen G.;
RT "Is AKR2A an essential molecular chaperone for a class of membrane-bound
RT proteins in plants?";
RL Plant Signal. Behav. 5:1520-1522(2010).
RN [10]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=21730198; DOI=10.1104/pp.111.178681;
RA Kim D.H., Xu Z.-Y., Na Y.J., Yoo Y.-J., Lee J., Sohn E.-J., Hwang I.;
RT "Small heat shock protein Hsp17.8 functions as an AKR2A cofactor in the
RT targeting of chloroplast outer membrane proteins in Arabidopsis.";
RL Plant Physiol. 157:132-146(2011).
CC -!- SUBUNIT: Interacts with AKR2A. {ECO:0000269|PubMed:18193034}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000269|PubMed:11595795, ECO:0000269|PubMed:17337631,
CC ECO:0000269|PubMed:18715957, ECO:0000269|PubMed:21730198}; Single-pass
CC membrane protein {ECO:0000269|PubMed:11595795}. Note=The targeting to
CC chloroplasts is facilitated by AKR2A and HSP17.8.
CC {ECO:0000269|PubMed:18193034, ECO:0000269|PubMed:21730198}.
CC -!- TISSUE SPECIFICITY: Confined to green tissues.
CC {ECO:0000269|PubMed:11595795}.
CC -!- DOMAIN: The transmembrane plays critical roles in migration to the
CC chloroplasts and/or subsequent insertion into the membrane.
CC {ECO:0000269|PubMed:11595795}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL050300; CAB43440.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78944.1; -; Genomic_DNA.
DR EMBL; BT010511; AAQ65134.1; -; mRNA.
DR EMBL; AK175275; BAD43038.1; -; mRNA.
DR EMBL; AK175812; BAD43575.1; -; mRNA.
DR PIR; T08457; T08457.
DR RefSeq; NP_190810.1; NM_115102.3.
DR AlphaFoldDB; Q9SVC4; -.
DR SMR; Q9SVC4; -.
DR STRING; 3702.AT3G52420.1; -.
DR iPTMnet; Q9SVC4; -.
DR PaxDb; Q9SVC4; -.
DR PRIDE; Q9SVC4; -.
DR ProteomicsDB; 238921; -.
DR EnsemblPlants; AT3G52420.1; AT3G52420.1; AT3G52420.
DR GeneID; 824407; -.
DR Gramene; AT3G52420.1; AT3G52420.1; AT3G52420.
DR KEGG; ath:AT3G52420; -.
DR Araport; AT3G52420; -.
DR TAIR; locus:2079929; AT3G52420.
DR eggNOG; ENOG502S8H4; Eukaryota.
DR HOGENOM; CLU_187971_0_0_1; -.
DR InParanoid; Q9SVC4; -.
DR OMA; WWTIEIA; -.
DR OrthoDB; 1644858at2759; -.
DR PhylomeDB; Q9SVC4; -.
DR PRO; PR:Q9SVC4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SVC4; baseline and differential.
DR Genevisible; Q9SVC4; AT.
DR GO; GO:0009707; C:chloroplast outer membrane; IDA:UniProtKB.
DR GO; GO:0031359; C:integral component of chloroplast outer membrane; IDA:TAIR.
DR InterPro; IPR038944; OEP7-like.
DR PANTHER; PTHR33982; PTHR33982; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Plastid outer membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..64
FT /note="Outer envelope membrane protein 7"
FT /id="PRO_0000434612"
FT TOPO_DOM 1..11
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000305|PubMed:11595795"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:11595795"
FT REGION 39..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 29..35
FT /note="AKR2A-binding sequence (ABS) required for
FT chloroplast outer envelope membrane targeting"
FT /evidence="ECO:0000269|PubMed:11595795,
FT ECO:0000269|PubMed:21057222"
FT MUTAGEN 10..21
FT /note="ATVVVAAMALGW->IIIIIIIIIIII: Normal targeting to the
FT chloroplast."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 10..21
FT /note="ATVVVAAMALGW->MAMMMMMMAMMM,FFFFFFFFFFFF: Impaired
FT targeting to the chloroplast resulting in the formation of
FT large aggregates in the cytoplasm."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 11..22
FT /note="TVVVAAMALGWL->AAAAAAAAAAAA,IIIIIIIIIIIIL: Normal
FT targeting to the chloroplast."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 15
FT /note="A->P: Reduced efficiency of chloroplast-targeting."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 19
FT /note="L->P: Impaired chloroplast-targeting."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 23
FT /note="A->P: Reduced efficiency of chloroplast-targeting."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 29
FT /note="K->G: Impaired targeting to the chloroplast outer
FT membrane leading to plasma membrane localization; when
FT associated with G-34."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 33
FT /note="D->G: Impaired targeting to the chloroplast outer
FT membrane leading to plasma membrane localization."
FT /evidence="ECO:0000269|PubMed:11595795"
FT MUTAGEN 34
FT /note="K->G: Impaired targeting to the chloroplast outer
FT membrane leading to plasma membrane localization; when
FT associated with G-29."
FT /evidence="ECO:0000269|PubMed:11595795"
SQ SEQUENCE 64 AA; 6764 MW; BA5B5D4340F9B006 CRC64;
MGKTSGAKQA TVVVAAMALG WLAIEIAFKP FLDKFRSSID KSDPTKDPDD FDTAATATTS
KEGL
