OEP80_ARATH
ID OEP80_ARATH Reviewed; 732 AA.
AC Q9C5J8;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Outer envelope protein 80, chloroplastic;
DE AltName: Full=Chloroplastic outer envelope protein of 80 kDa;
DE Short=AtOEP80;
DE AltName: Full=Protein TOC75-V;
DE Short=AtToc75-V;
GN Name=OEP80; OrderedLocusNames=At5g19620; ORFNames=T29J13.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION, AND SUBCELLULAR LOCATION.
RX PubMed=12034753; DOI=10.1093/embo-reports/kvf110;
RA Eckart K., Eichacker L., Sohrt K., Schleiff E., Heins L., Soll J.;
RT "A Toc75-like protein import channel is abundant in chloroplasts.";
RL EMBO Rep. 3:557-562(2002).
RN [5]
RP SUBCELLULAR LOCATION, AND LACK OF PROCESSING.
RX PubMed=15255865; DOI=10.1111/j.1365-313x.2004.02135.x;
RA Inoue K., Potter D.;
RT "The chloroplastic protein translocation channel Toc75 and its paralog
RT OEP80 represent two distinct protein families and are targeted to the
RT chloroplastic outer envelope by different mechanisms.";
RL Plant J. 39:354-365(2004).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, LACK OF PROCESSING, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18621981; DOI=10.1104/pp.108.122754;
RA Patel R., Hsu S.-C., Bedard J., Inoue K., Jarvis P.;
RT "The Omp85-related chloroplast outer envelope protein OEP80 is essential
RT for viability in Arabidopsis.";
RL Plant Physiol. 148:235-245(2008).
CC -!- FUNCTION: Plays an essential role during early stages of plastid
CC development. {ECO:0000269|PubMed:18621981}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000269|PubMed:12034753, ECO:0000269|PubMed:15255865}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:18621981}.
CC -!- DOMAIN: The N-terminal domain (1-52) is not required for import,
CC membrane integration or activity.
CC -!- DISRUPTION PHENOTYPE: Embryo lethality at the globular stage.
CC {ECO:0000269|PubMed:18621981}.
CC -!- MISCELLANEOUS: Probably not processed upon targeting to chloroplasts.
CC The targeting is independent of the general import pathway.
CC -!- SIMILARITY: Belongs to the OEP80 (TC 1.B.33.2) family. {ECO:0000305}.
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DR EMBL; AF296838; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92731.1; -; Genomic_DNA.
DR EMBL; AF360205; AAK25915.1; -; mRNA.
DR EMBL; AY040053; AAK64111.1; -; mRNA.
DR RefSeq; NP_568378.1; NM_121967.4.
DR AlphaFoldDB; Q9C5J8; -.
DR SMR; Q9C5J8; -.
DR BioGRID; 17358; 8.
DR STRING; 3702.AT5G19620.1; -.
DR TCDB; 1.B.33.2.2; the outer membrane protein insertion porin (bam complex) (ompip) family.
DR SwissPalm; Q9C5J8; -.
DR PaxDb; Q9C5J8; -.
DR PRIDE; Q9C5J8; -.
DR ProteomicsDB; 238922; -.
DR EnsemblPlants; AT5G19620.1; AT5G19620.1; AT5G19620.
DR GeneID; 832082; -.
DR Gramene; AT5G19620.1; AT5G19620.1; AT5G19620.
DR KEGG; ath:AT5G19620; -.
DR Araport; AT5G19620; -.
DR TAIR; locus:2183164; AT5G19620.
DR eggNOG; ENOG502QSQ3; Eukaryota.
DR HOGENOM; CLU_007664_2_1_1; -.
DR InParanoid; Q9C5J8; -.
DR OMA; PPYEAFC; -.
DR OrthoDB; 265672at2759; -.
DR PhylomeDB; Q9C5J8; -.
DR PRO; PR:Q9C5J8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9C5J8; baseline and differential.
DR Genevisible; Q9C5J8; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0001401; C:SAM complex; IBA:GO_Central.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IBA:GO_Central.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IBA:GO_Central.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR039910; D15-like.
DR InterPro; IPR034746; POTRA.
DR PANTHER; PTHR12815; PTHR12815; 1.
DR Pfam; PF01103; Omp85; 1.
DR PROSITE; PS51779; POTRA; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Plastid outer membrane; Reference proteome.
FT CHAIN 1..732
FT /note="Outer envelope protein 80, chloroplastic"
FT /id="PRO_0000352174"
FT DOMAIN 314..396
FT /note="POTRA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 732 AA; 79936 MW; 42D2F84E68C4BF83 CRC64;
MHCHNDDVRF SSSSIRIHSP SPKEQHSLLT NLQSCSKTFV SHLSNTRNSL NQMLQSLKNR
HTPPPRSVRR PNLPTQMLNS VTQLMIGKSS PISLSLIQST QFNWSESRDE NVETIRGLSS
PLLCCASLSL TRPNESTQSV EGKDTVQQQK GHSVSRNAEE RVLISEVLVR TKDGEELERK
DLEMEALAAL KACRANSALT IREVQEDVHR IIESGYFCSC TPVAVDTRDG IRLMFQVEPN
QEFRGLVCEN ANVLPSKFIH EAFRDGFGKV INIKRLEEAI TSINGWYMER GLFGIVSDID
TLSGGIVRLQ VAEAEVNNIS IRFLDRKTGE PTKGKTSPET ILRQLTTKKG QVYSMLQGKR
DVDTVLAMGI MEDVSIIPQP AGDSGKVDLI MNCVERPSGG FSAGGGISSG ITSGPLSGLI
GSFAYSHRNL FGRNQKLNVS LERGQIDSIF RINYTDPWIE GDDKRTSRSI MVQNSRTPGN
LVHGNQPDNS SLTIGRVTAG VEYSRPFRPK WNGTAGLIFQ HAGARDEQGN PIIKDFYSSP
LTASGKPHDE TMLAKLESIY TGSGDQGSTM FAFNMEQGLP VLPEWLCFNR VTGRARKGIH
IGPARFLFSL SGGHVVGKFS PHEAFVIGGT NSVRGYEEGA VGSGRSYVVG SGELSFPVRG
PVEGVIFTDY GTDMGSGSTV PGDPAGARLK PGSGYGYGLG VRVDSPLGPL RLEYAFNDQH
AGRFHFGVGL RN
