OFP1_ARATH
ID OFP1_ARATH Reviewed; 270 AA.
AC Q9LZW2;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Transcription repressor OFP1;
DE AltName: Full=Ovate family protein 1;
DE Short=AtOFP1;
GN Name=OFP1; OrderedLocusNames=At5g01840; ORFNames=T20L15.110;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, INTERACTION WITH ATH1; BLH1; BLH2; BLH3; BLH4; BLH5; BLH10;
RP KNAT1; KNAT3; KNAT4; KNAT5 AND KNAT7, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15781858; DOI=10.1073/pnas.0501181102;
RA Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.;
RT "A central role of Arabidopsis thaliana ovate family proteins in networking
RT and subcellular localization of 3-aa loop extension homeodomain proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17461792; DOI=10.1111/j.1365-313x.2007.03096.x;
RA Wang S., Chang Y., Guo J., Chen J.G.;
RT "Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that
RT suppresses cell elongation.";
RL Plant J. 50:858-872(2007).
RN [5]
RP FUNCTION, INTERACTION WITH KU70, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20844935; DOI=10.1007/s11103-010-9685-5;
RA Wang Y.K., Chang W.C., Liu P.F., Hsiao M.K., Lin C.T., Lin S.M., Pan R.L.;
RT "Ovate family protein 1 as a plant Ku70 interacting protein involving in
RT DNA double-strand break repair.";
RL Plant Mol. Biol. 74:453-466(2010).
RN [6]
RP FUNCTION, INTERACTION WITH KNAT7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=21457372; DOI=10.1111/j.1365-313x.2011.04595.x;
RA Li E., Wang S., Liu Y., Chen J.G., Douglas C.J.;
RT "OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary
RT cell wall formation in Arabidopsis thaliana.";
RL Plant J. 67:328-341(2011).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, AND DISRUPTION PHENOTYPE.
RX PubMed=21886836; DOI=10.1371/journal.pone.0023896;
RA Wang S., Chang Y., Guo J., Zeng Q., Ellis B.E., Chen J.G.;
RT "Arabidopsis ovate family proteins, a novel transcriptional repressor
RT family, control multiple aspects of plant growth and development.";
RL PLoS ONE 6:E23896-E23896(2011).
CC -!- FUNCTION: Transcriptional repressor that regulates multiple aspects of
CC plant growth and development through the regulation of BEL1-LIKE (BLH)
CC and KNOX TALE (KNAT) homeodomain transcription factors. Controls the
CC subcellular localization of the homeodomain protein BLH1. Plays a role
CC in the regulation of cell elongation by controlling the expression of
CC GA20OX1, a gene that encodes a key enzyme in gibberellin biosynthesis.
CC May play a role in double-stranded DNA repair through the DNA non-
CC homologous end joining (NHEJ) pathway along with KU70 and KU80 protein
CC complex. Possesses DNA-binding activity towards double-stranded and
CC single-stranded DNA in vitro. {ECO:0000269|PubMed:15781858,
CC ECO:0000269|PubMed:17461792, ECO:0000269|PubMed:20844935,
CC ECO:0000269|PubMed:21457372, ECO:0000269|PubMed:21886836}.
CC -!- SUBUNIT: Interacts with ATH1, BLH1, BLH2, BLH3, BLH4, BLH5, BLH10,
CC KNAT1, KNAT3, KNAT4, KNAT5, KNAT7 and KU70.
CC {ECO:0000269|PubMed:15781858, ECO:0000269|PubMed:20844935,
CC ECO:0000269|PubMed:21457372}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15781858,
CC ECO:0000269|PubMed:17461792, ECO:0000269|PubMed:21457372}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, xylem of stems, flower buds and
CC siliques. {ECO:0000269|PubMed:17461792, ECO:0000269|PubMed:21457372,
CC ECO:0000269|PubMed:21886836}.
CC -!- INDUCTION: By the DNA-damaging agents methyl methanesulfonate (MMS) and
CC menadione. {ECO:0000269|PubMed:20844935}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants are hypersensitive to the DNA-damaging
CC agents methyl methanesulfonate (MMS) and menadione.
CC {ECO:0000269|PubMed:15781858, ECO:0000269|PubMed:17461792,
CC ECO:0000269|PubMed:20844935, ECO:0000269|PubMed:21457372,
CC ECO:0000269|PubMed:21886836}.
CC -!- MISCELLANEOUS: Plants over-expressing OFP1 show stunted growth with
CC general delayed development and shortening and thickening of all aerial
CC parts. Leaves are irregularly heart-shaped and lobed and display curved
CC surfaces. Anthers are short with a thick filament and style and stigma
CC protruded from the flower. Siliques are short and uneven and produce
CC few seeds (PubMed:15781858 and PubMed:21886836).
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DR EMBL; AL162351; CAB82754.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90399.1; -; Genomic_DNA.
DR PIR; T48205; T48205.
DR RefSeq; NP_195804.1; NM_120262.3.
DR AlphaFoldDB; Q9LZW2; -.
DR SMR; Q9LZW2; -.
DR BioGRID; 17089; 16.
DR IntAct; Q9LZW2; 13.
DR STRING; 3702.AT5G01840.1; -.
DR PaxDb; Q9LZW2; -.
DR PRIDE; Q9LZW2; -.
DR ProteomicsDB; 250887; -.
DR EnsemblPlants; AT5G01840.1; AT5G01840.1; AT5G01840.
DR GeneID; 831813; -.
DR Gramene; AT5G01840.1; AT5G01840.1; AT5G01840.
DR KEGG; ath:AT5G01840; -.
DR Araport; AT5G01840; -.
DR TAIR; locus:2180977; AT5G01840.
DR eggNOG; ENOG502RTS2; Eukaryota.
DR HOGENOM; CLU_036558_0_0_1; -.
DR InParanoid; Q9LZW2; -.
DR OMA; CLNSDEY; -.
DR OrthoDB; 1209787at2759; -.
DR PhylomeDB; Q9LZW2; -.
DR PRO; PR:Q9LZW2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZW2; baseline and differential.
DR Genevisible; Q9LZW2; AT.
DR GO; GO:0005856; C:cytoskeleton; IDA:TAIR.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0043622; P:cortical microtubule organization; IMP:TAIR.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0051510; P:regulation of unidimensional cell growth; IMP:TAIR.
DR InterPro; IPR025830; DNA_bnd_dom_ovate.
DR InterPro; IPR038933; Ovate.
DR InterPro; IPR006458; Ovate_C.
DR PANTHER; PTHR33057; PTHR33057; 1.
DR Pfam; PF13724; DNA_binding_2; 1.
DR Pfam; PF04844; Ovate; 1.
DR TIGRFAMs; TIGR01568; A_thal_3678; 1.
DR PROSITE; PS51754; OVATE; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; DNA-binding; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..270
FT /note="Transcription repressor OFP1"
FT /id="PRO_0000429670"
FT DOMAIN 202..261
FT /note="OVATE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01090"
FT REGION 25..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 28..45
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 260..264
FT /note="LxLxL"
FT COMPBIAS 30..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..72
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..114
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..195
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 270 AA; 30030 MW; C2A2058809257C39 CRC64;
MGNNYRFKLS ELIPNAWFYK LRDMSKSKKK NLQSQPNSTT SKKKHHAVPT PTSTTPLSPR
PPRRPSHSSK APPSHPPRKS SGNRLRHRAT VDSKSSTTSG DSTTTETGSF SPDFRSDQVL
LPDESLTGSW HSPCSSKLSK TATFTPPPEL ELRPIITKTA ATARKTAVNS PAGVRLRMRS
PRISVSSSAR RSGSSARRSR AVVKASVDPK RDFKESMEEM IAENKIRATK DLEELLACYL
CLNSDEYHAI IINVFKQIWL DLNLPPPHSK
