OFP8_ORYSJ
ID OFP8_ORYSJ Reviewed; 228 AA.
AC Q94CV1; Q0JHH3;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Transcription repressor OFP8 {ECO:0000305};
DE AltName: Full=OVATE family protein 8 {ECO:0000303|PubMed:27332964};
DE Short=OsOFP8 {ECO:0000303|PubMed:27332964};
GN Name=OFP8 {ECO:0000303|PubMed:27332964};
GN OrderedLocusNames=Os01g0864000 {ECO:0000312|EMBL:BAS75362.1},
GN LOC_Os01g64430 {ECO:0000305};
GN ORFNames=OsJ_04171 {ECO:0000312|EMBL:EAZ14247.1},
GN P0423B08.30 {ECO:0000312|EMBL:BAB63811.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, INTERACTION WITH GSK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INDUCTION BY 24-EPIBRASSINOLIDE, AND PHOSPHORYLATION.
RX PubMed=27332964; DOI=10.1371/journal.pgen.1006118;
RA Yang C., Shen W., He Y., Tian Z., Li J.;
RT "OVATE family protein 8 positively mediates brassinosteroid signaling
RT through interacting with the GSK3-like kinase in rice.";
RL PLoS Genet. 12:E1006118-E1006118(2016).
CC -!- FUNCTION: Probable transcriptional repressor that regulates multiple
CC aspects of plant growth and development, partly through brassinosteroid
CC (BR) signaling pathway. Acts downstream of the kinase GSK2, a negative
CC regulator of BR signaling. {ECO:0000269|PubMed:27332964}.
CC -!- SUBUNIT: Interacts with GSK2. {ECO:0000269|PubMed:27332964}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27332964}. Cytoplasm
CC {ECO:0000269|PubMed:27332964}. Note=Phosphorylated OFP8 shuttles from
CC the nucleus to the cytoplasm where it is degraded by the proteasome.
CC {ECO:0000269|PubMed:27332964}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, stem nodes, young
CC leaves, leaf sheaths, lamina joints, young spikelets, inflorescences,
CC stamens and ovaries, embryos and seeds. {ECO:0000269|PubMed:27332964}.
CC -!- INDUCTION: Induced by 24-epibrassinolide.
CC {ECO:0000269|PubMed:27332964}.
CC -!- PTM: Phosphorylated on serine and threonine residues by GSK2.
CC Dephosphorylated during response to brassinosteroid.
CC {ECO:0000269|PubMed:27332964}.
CC -!- MISCELLANEOUS: The gain-of-function mutant ofp8 (T-DNA tagging) shows
CC an enhanced lamina joint inclination at the maturation stage. Plants
CC silencing OFP8 have a marked upright leaf phenotype and are insensitive
CC to brassinolide. {ECO:0000269|PubMed:27332964}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF06805.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP003611; BAB63811.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06805.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; BAS75362.1; -; Genomic_DNA.
DR EMBL; CM000138; EAZ14247.1; -; Genomic_DNA.
DR EMBL; AK106759; BAG97821.1; -; mRNA.
DR RefSeq; XP_015643359.1; XM_015787873.1.
DR AlphaFoldDB; Q94CV1; -.
DR SMR; Q94CV1; -.
DR STRING; 4530.OS01T0864000-01; -.
DR PaxDb; Q94CV1; -.
DR PRIDE; Q94CV1; -.
DR EnsemblPlants; Os01t0864000-01; Os01t0864000-01; Os01g0864000.
DR GeneID; 4324787; -.
DR Gramene; Os01t0864000-01; Os01t0864000-01; Os01g0864000.
DR KEGG; osa:4324787; -.
DR eggNOG; ENOG502S0X5; Eukaryota.
DR HOGENOM; CLU_061898_2_0_1; -.
DR InParanoid; Q94CV1; -.
DR OMA; WGPASFV; -.
DR OrthoDB; 1501951at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:1900457; P:regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR InterPro; IPR038933; Ovate.
DR InterPro; IPR006458; Ovate_C.
DR PANTHER; PTHR33057; PTHR33057; 1.
DR Pfam; PF04844; Ovate; 1.
DR TIGRFAMs; TIGR01568; A_thal_3678; 1.
DR PROSITE; PS51754; OVATE; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; Cytoplasm; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..228
FT /note="Transcription repressor OFP8"
FT /id="PRO_0000439017"
FT DOMAIN 157..216
FT /note="OVATE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01090"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 36..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 228 AA; 25360 MW; D45B94D58CC186C3 CRC64;
MSGRSSRRGS FSLRQPPVVD IGCNCRRPKL FSIFSSSSSS SFRRGGSKPK SPNASSTSTT
TAFTATTGGA GTATSTDSSW GPASFTTNSL FEEPAAAQQE QEQLETRRRR RQQRRRRRRA
GATSFARGGD VGGHDDEQQQ LQEQAPYRRV AKESVAVAVE SAEPYEDFRE SMVQMVVEKE
IYAWDDLNDL LHQFLSLNSP RHHPLILHAF ADLWTRNGLF SPPSPCQF
