ID   ARSR1_PSEPK             Reviewed;         128 AA.
AC   Q88LK1;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Arsenic resistance transcriptional regulator ArsR1 {ECO:0000305};
GN   Name=arsR1 {ECO:0000303|PubMed:27208139};
GN   Synonyms=arsR-I {ECO:0000312|EMBL:AAN67547.1};
GN   OrderedLocusNames=PP_1930 {ECO:0000312|EMBL:AAN67547.1};
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
RN   [2]
RP   FUNCTION, DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=27208139; DOI=10.1128/aem.00606-16;
RA   Fernandez M., Morel B., Ramos J.L., Krell T.;
RT   "Paralogous regulators ArsR1 and ArsR2 of Pseudomonas putida KT2440 as a
RT   basis for arsenic biosensor development.";
RL   Appl. Environ. Microbiol. 82:4133-4144(2016).
CC   -!- FUNCTION: Binds arsenite and regulates the expression of arsenic efflux
CC       pumps. In vitro, also binds antimony and bismuth, but not arsenate.
CC       {ECO:0000269|PubMed:27208139}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:27208139}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27208139}.
CC   -!- BIOTECHNOLOGY: Could be used to construct robust and highly sensitive
CC       arsenic biosensors. {ECO:0000269|PubMed:27208139}.
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DR   EMBL; AE015451; AAN67547.1; -; Genomic_DNA.
DR   RefSeq; NP_744083.1; NC_002947.4.
DR   AlphaFoldDB; Q88LK1; -.
DR   SMR; Q88LK1; -.
DR   STRING; 160488.PP_1930; -.
DR   PRIDE; Q88LK1; -.
DR   EnsemblBacteria; AAN67547; AAN67547; PP_1930.
DR   KEGG; ppu:PP_1930; -.
DR   PATRIC; fig|160488.4.peg.2041; -.
DR   eggNOG; COG0640; Bacteria.
DR   HOGENOM; CLU_097806_3_1_6; -.
DR   OMA; DGREHCV; -.
DR   PhylomeDB; Q88LK1; -.
DR   BioCyc; PPUT160488:G1G01-2045-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01022; HTH_5; 1.
DR   PRINTS; PR00778; HTHARSR.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   1: Evidence at protein level;
KW   Arsenical resistance; Cytoplasm; DNA-binding; Metal-binding;
KW   Metal-thiolate cluster; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..128
FT                   /note="Arsenic resistance transcriptional regulator ArsR1"
FT                   /id="PRO_0000438512"
FT   DOMAIN          11..103
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        44..67
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         43
FT                   /ligand="arsenite"
FT                   /ligand_id="ChEBI:CHEBI:29242"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340,
FT                   ECO:0000305"
FT   BINDING         45
FT                   /ligand="arsenite"
FT                   /ligand_id="ChEBI:CHEBI:29242"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340,
FT                   ECO:0000305"
SQ   SEQUENCE   128 AA;  14487 MW;  29A5C5AA11532BC9 CRC64;
     MAVRAFPGGH MREILTPPIV FKCLADDTRA RMTLLIAREG ELCVCELTHA LELSQPKISR
     HLAQLREAGI LMDRRKGQWV YYRLHPEVPQ WVDAMLKGVV DANQEWLSPD ALRLAEMGER
     PQSPVACA