OFUT5_ARATH
ID OFUT5_ARATH Reviewed; 564 AA.
AC Q84WU0; Q9SHI5;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=O-fucosyltransferase 5 {ECO:0000305};
DE Short=O-FucT-5 {ECO:0000305};
DE EC=2.4.1.- {ECO:0000305};
DE AltName: Full=O-fucosyltransferase family protein {ECO:0000305};
GN Name=OFUT5 {ECO:0000305};
GN Synonyms=GT68 {ECO:0000303|PubMed:23272088, ECO:0000312|EMBL:AHL38942.1};
GN OrderedLocusNames=At1g17270 {ECO:0000312|Araport:AT1G17270};
GN ORFNames=F20D23.3 {ECO:0000312|EMBL:AAD50008.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND WEB RESOURCE.
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP REVIEW.
RX PubMed=22629278; DOI=10.3389/fpls.2012.00059;
RA Hansen S.F., Harholt J., Oikawa A., Scheller H.V.;
RT "Plant glycosyltransferases beyond CAZy: a perspective on DUF families.";
RL Front. Plant Sci. 3:59-59(2012).
RN [6]
RP GENE FAMILY.
RX PubMed=23272088; DOI=10.1371/journal.pone.0051129;
RA Voxeur A., Andre A., Breton C., Lerouge P.;
RT "Identification of putative rhamnogalacturonan-II specific
RT glycosyltransferases in Arabidopsis using a combination of bioinformatics
RT approaches.";
RL PLoS ONE 7:E51129-E51129(2012).
RN [7]
RP GENE FAMILY.
RX PubMed=22966747; DOI=10.1111/tpj.12019;
RA Wang Y., Mortimer J.C., Davis J., Dupree P., Keegstra K.;
RT "Identification of an additional protein involved in mannan biosynthesis.";
RL Plant J. 73:105-117(2013).
CC -!- PATHWAY: Glycan metabolism. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase GT106 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD50008.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=The Arabidopsis GT Collection;
CC URL="http://gt.jbei.org/arabidopsis.html";
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DR EMBL; KJ139002; AHL38942.1; -; mRNA.
DR EMBL; AC007651; AAD50008.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29566.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60393.1; -; Genomic_DNA.
DR EMBL; BT002770; AAO22598.1; -; mRNA.
DR PIR; A86309; A86309.
DR RefSeq; NP_001322684.1; NM_001332286.1.
DR RefSeq; NP_173170.2; NM_101588.4.
DR AlphaFoldDB; Q84WU0; -.
DR SMR; Q84WU0; -.
DR STRING; 3702.AT1G17270.1; -.
DR iPTMnet; Q84WU0; -.
DR PaxDb; Q84WU0; -.
DR PRIDE; Q84WU0; -.
DR ProteomicsDB; 250801; -.
DR EnsemblPlants; AT1G17270.1; AT1G17270.1; AT1G17270.
DR EnsemblPlants; AT1G17270.2; AT1G17270.2; AT1G17270.
DR GeneID; 838298; -.
DR Gramene; AT1G17270.1; AT1G17270.1; AT1G17270.
DR Gramene; AT1G17270.2; AT1G17270.2; AT1G17270.
DR KEGG; ath:AT1G17270; -.
DR Araport; AT1G17270; -.
DR TAIR; locus:2020432; AT1G17270.
DR eggNOG; ENOG502QS4M; Eukaryota.
DR HOGENOM; CLU_039118_0_0_1; -.
DR InParanoid; Q84WU0; -.
DR OMA; VEKKWVM; -.
DR OrthoDB; 459621at2759; -.
DR PhylomeDB; Q84WU0; -.
DR PRO; PR:Q84WU0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84WU0; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046922; F:peptide-O-fucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006004; P:fucose metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR019378; GDP-Fuc_O-FucTrfase.
DR InterPro; IPR045130; OFUT2-like.
DR PANTHER; PTHR13398; PTHR13398; 1.
DR Pfam; PF10250; O-FucT; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Fucose metabolism; Glycoprotein;
KW Glycosyltransferase; Membrane; Reference proteome; Signal-anchor;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..564
FT /note="O-fucosyltransferase 5"
FT /id="PRO_0000442068"
FT TRANSMEM 70..90
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000305"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 413..415
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2G5"
FT BINDING 529..530
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2G5"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 564 AA; 63622 MW; 3B59E16A878B2AE1 CRC64;
MVRNSSDEEE DHRNLIPQND TRDNDLNLRP DARTVNMANG GGRSPRSALQ IDEILSRARN
RWKISVNKRY VVAAVSLTLF VGLLFLFTDT RTFFSSFKLD PMSSRVKESE LQALNLLRQQ
QLALVSLLNR TNFNSSNAIS SSVVIDNVKA ALLKQISVNK EIEEVLLSPH RTGNYSITAS
GSDSFTGSYN ADICRKVDQK LLDRKTIEWK PRPDKFLFAI CLSGQMSNHL ICLEKHMFFA
ALLDRVLVIP SSKFDYQYDK VIDIERINTC LGRTVVISFD QFKEIDKKNN AHIDRFICYV
SSPQPCYVDE DHIKKLKGLG VSIGGKLEAP WSEDIKKPTK RTSQEVVEKF KSDDGVIAIG
DVFYADMEQD LVMQPGGPIN HKCKTLIEPS RLILVTAQRF IQTFLGKNFI SLHLRRHGFL
KFCNAKSPSC FYPIPQAADC ISRMVERANA PVIYLSTDAA ESETGLLQSL VVVDGKVVPL
VKRPPQNSAE KWDSLLYRHG IEDDSQVYAM LDKTICAMSS VFIGASGSTF TEDILRLRKD
WGTSSMCDEY LCRGEEPNFI AENE
