OFUT8_ARATH
ID OFUT8_ARATH Reviewed; 611 AA.
AC F4HZX7; Q5BPY9; Q9LP49;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=O-fucosyltransferase 8 {ECO:0000305};
DE Short=O-FucT-8 {ECO:0000305};
DE EC=2.4.1.- {ECO:0000305};
DE AltName: Full=O-fucosyltransferase family protein {ECO:0000312|EMBL:ARJ31409.1};
GN Name=OFUT8 {ECO:0000305};
GN OrderedLocusNames=At1g29200 {ECO:0000312|Araport:AT1G29200};
GN ORFNames=F28N24.11 {ECO:0000312|EMBL:AAF88118.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Zeng W., Gluza P., Heazlewood J.;
RT "Arabidopsis glycosyltransferases: an update.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Xiao Y., Underwood B.A., Moskal W., Redman J., Wang W., Monaghan E.,
RA Wu H.C., Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND REVIEW.
RX PubMed=22629278; DOI=10.3389/fpls.2012.00059;
RA Hansen S.F., Harholt J., Oikawa A., Scheller H.V.;
RT "Plant glycosyltransferases beyond CAZy: a perspective on DUF families.";
RL Front. Plant Sci. 3:59-59(2012).
RN [7]
RP GENE FAMILY.
RX PubMed=22916179; DOI=10.1371/journal.pone.0042914;
RA Neumetzler L., Humphrey T., Lumba S., Snyder S., Yeats T.H., Usadel B.,
RA Vasilevski A., Patel J., Rose J.K., Persson S., Bonetta D.;
RT "The FRIABLE1 gene product affects cell adhesion in Arabidopsis.";
RL PLoS ONE 7:E42914-E42914(2012).
RN [8]
RP GENE FAMILY.
RX PubMed=23272088; DOI=10.1371/journal.pone.0051129;
RA Voxeur A., Andre A., Breton C., Lerouge P.;
RT "Identification of putative rhamnogalacturonan-II specific
RT glycosyltransferases in Arabidopsis using a combination of bioinformatics
RT approaches.";
RL PLoS ONE 7:E51129-E51129(2012).
RN [9]
RP GENE FAMILY.
RX PubMed=22966747; DOI=10.1111/tpj.12019;
RA Wang Y., Mortimer J.C., Davis J., Dupree P., Keegstra K.;
RT "Identification of an additional protein involved in mannan biosynthesis.";
RL Plant J. 73:105-117(2013).
RN [10]
RP WEB RESOURCE.
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
CC -!- PATHWAY: Glycan metabolism. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4HZX7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HZX7-2; Sequence=VSP_059167;
CC -!- SIMILARITY: Belongs to the glycosyltransferase GT106 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF88118.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=The Arabidopsis GT Collection;
CC URL="http://gt.jbei.org/arabidopsis.html";
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DR EMBL; KY906045; ARJ31409.1; -; mRNA.
DR EMBL; AC021043; AAF88118.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31059.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31058.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59998.1; -; Genomic_DNA.
DR EMBL; AY924689; AAX23764.1; -; mRNA.
DR EMBL; DQ132669; AAZ52699.1; -; mRNA.
DR PIR; E86414; E86414.
DR RefSeq; NP_001117377.1; NM_001123905.1. [F4HZX7-1]
DR RefSeq; NP_001322313.1; NM_001332841.1. [F4HZX7-2]
DR RefSeq; NP_174215.2; NM_102661.3. [F4HZX7-2]
DR AlphaFoldDB; F4HZX7; -.
DR PaxDb; F4HZX7; -.
DR PRIDE; F4HZX7; -.
DR ProteomicsDB; 238938; -. [F4HZX7-1]
DR EnsemblPlants; AT1G29200.1; AT1G29200.1; AT1G29200. [F4HZX7-2]
DR EnsemblPlants; AT1G29200.2; AT1G29200.2; AT1G29200. [F4HZX7-1]
DR EnsemblPlants; AT1G29200.4; AT1G29200.4; AT1G29200. [F4HZX7-2]
DR GeneID; 839795; -.
DR Gramene; AT1G29200.1; AT1G29200.1; AT1G29200. [F4HZX7-2]
DR Gramene; AT1G29200.2; AT1G29200.2; AT1G29200. [F4HZX7-1]
DR Gramene; AT1G29200.4; AT1G29200.4; AT1G29200. [F4HZX7-2]
DR KEGG; ath:AT1G29200; -.
DR Araport; AT1G29200; -.
DR TAIR; locus:2029984; AT1G29200.
DR eggNOG; ENOG502QVVB; Eukaryota.
DR InParanoid; F4HZX7; -.
DR OMA; KPCAAKH; -.
DR OrthoDB; 526720at2759; -.
DR PRO; PR:F4HZX7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HZX7; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006004; P:fucose metabolic process; IEA:UniProtKB-KW.
DR CDD; cd11299; O-FucT_plant; 1.
DR InterPro; IPR024709; FucosylTrfase_pln.
DR InterPro; IPR019378; GDP-Fuc_O-FucTrfase.
DR Pfam; PF10250; O-FucT; 1.
DR PIRSF; PIRSF009360; UCP009360; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Carbohydrate metabolism; Fucose metabolism;
KW Glycoprotein; Glycosyltransferase; Membrane; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..611
FT /note="O-fucosyltransferase 8"
FT /id="PRO_0000442071"
FT TRANSMEM 78..98
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000305"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 386..388
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9H488"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 216
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 270
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 506
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1..119
FT /note="MGKQGSPRSPRPETIDKEEKFGRRSLDSLSGNDLLLGRRIYASEVSKAQGSK
FT HDQSSGSSNKFWKKQHSWLRRNFKSIVLMISVTGFIFCMDSIMVSIFHSDSRAVVQDIS
FT RLSNMTLH -> MSI (in isoform 2)"
FT /id="VSP_059167"
SQ SEQUENCE 611 AA; 69119 MW; 60C46F867D5DEE8B CRC64;
MGKQGSPRSP RPETIDKEEK FGRRSLDSLS GNDLLLGRRI YASEVSKAQG SKHDQSSGSS
NKFWKKQHSW LRRNFKSIVL MISVTGFIFC MDSIMVSIFH SDSRAVVQDI SRLSNMTLHK
NGAVDASPVQ MYSRLLNLAS DSLAKNEFKP DTPNFREERS SKSSQWKPCA DNNKAAVALE
RSRELSNGYI MVSANGGLNQ QRVAICNAVA VAALLNATLV LPRFLYSNVW KDPSQFGDIY
QEDHFIEYLK DEVNIVKNLP QHLKSTDNKN LSLVTDTELV KEATPVDYIE HVLPLLKKYG
MVHLFGYGNR LGFDPLPFDV QRLRCKCNFH ALKFAPKIQE AGSLLVKRIR RFKTSRSRLE
EALLGESMVK STVKGEEEPL KYLALHLRFE EDMVAYSLCD FGGGEAERKE LQAYREDHFP
LLLKRLKKSK PVSPEELRKT GKCPLTPEEA TLVLAGLGFK RKTYIYLAGS QIYGGSSRML
PLTRLYPNIA TKETLLTPQE LAPFKNFSSQ LAALDFIACI ASDVFAMTDS GSQLSSLVSG
FRNYYGNGQA PTLRPNKKRL AAILSDSETI KWKIFEDRVR KMVEEGQKLR TRPYGRSIYR
QPRCPECMCK F
