ID   A28_VACCC               Reviewed;         146 AA.
AC   P68632; P21086; Q80HU6;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Envelope protein A28 homolog;
GN   ORFNames=A28L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Envelope protein required for virus entry into host cell and
CC       for cell-cell fusion (syncytium formation). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex.
CC       Interacts directly with protein H2 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       III membrane protein {ECO:0000305}. Note=Component of the intracellular
CC       mature virion (IMV) outer membrane. {ECO:0000250}.
CC   -!- PTM: Contains two intramolecular disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific pathway
CC       that operates on the cytoplasmic side of the MV membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae A28 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48153.1; -; Genomic_DNA.
DR   PIR; C42520; C42520.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039663; P:membrane fusion involved in viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR007664; Poxvirus_A28.
DR   Pfam; PF04584; Pox_A28; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Fusion of virus membrane with host membrane; Late protein;
KW   Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral envelope protein;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..146
FT                   /note="Envelope protein A28 homolog"
FT                   /id="PRO_0000099299"
FT   TRANSMEM        1..21
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        22..146
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   146 AA;  16329 MW;  27C91A3A47DB0A0A CRC64;
     MNSLSIFFIV VATAAVCLLF IQGYSIYENY GNIKEFNATH AAFEYSKSIG GTPALDRRVQ
     DVNDTISDVK QKWRCVVYPG NGFVSASIFG FQAEVGPNNT RSIRKFNTMQ QCIDFTFSDV
     ININIYNPCV VPNINNAECQ FLKSVL