ID   OHK2_ORYSI              Reviewed;        1048 AA.
AC   A2YA15;
DT   16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Probable histidine kinase 2 {ECO:0000305};
DE            Short=OsHK2 {ECO:0000305};
DE            EC=2.7.13.3 {ECO:0000305};
GN   Name=HK2 {ECO:0000305}; Synonyms=OHK1 {ECO:0000305};
GN   ORFNames=OsI_21926 {ECO:0000312|EMBL:EAY99925.1};
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Cytokinin receptor related to bacterial two-component
CC       regulators. Functions as a histidine kinase and transmits the stress
CC       signal to a downstream MAPK cascade. {ECO:0000250|UniProtKB:A1A698}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC       active histidine that mediates the phosphotransfer. {ECO:0000305}.
CC   -!- PTM: Activation probably requires a transfer of a phosphate group
CC       between a His in the transmitter domain and an Asp of the receiver
CC       domain. {ECO:0000305}.
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DR   EMBL; CM000131; EAY99925.1; -; Genomic_DNA.
DR   AlphaFoldDB; A2YA15; -.
DR   SMR; A2YA15; -.
DR   STRING; 39946.A2YA15; -.
DR   EnsemblPlants; BGIOSGA021781-TA; BGIOSGA021781-PA; BGIOSGA021781.
DR   Gramene; BGIOSGA021781-TA; BGIOSGA021781-PA; BGIOSGA021781.
DR   HOGENOM; CLU_000445_104_16_1; -.
DR   OMA; NAGPFSE; -.
DR   Proteomes; UP000007015; Chromosome 6.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytokinin signaling pathway; Kinase; Membrane;
KW   Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..1048
FT                   /note="Probable histidine kinase 2"
FT                   /id="PRO_0000433806"
FT   TOPO_DOM        1..16
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        38..336
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        337..357
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        358..1048
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          398..662
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   DOMAIN          912..1044
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   MOD_RES         401
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         975
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   1048 AA;  114674 MW;  85A65F6B8D21DE85 CRC64;
     MREVEEVSKW RRRCCYFWIL FPLAVIATCM TITVVTFCSS TMYMTEVMGE ATKGAMDSAL
     MHIAGNMRPL LEANRSVFTI ANTLHVQGNM ASFSHVGPKL FLAFSMQPLQ AQISYAAVDG
     AAFAYYRAGG GDGEARAMFA RPNGTWFTQA VDPATGRPVG NATAAAPHQQ LPPNVTRLLL
     DGGGGGASLA DGWARPGVRM LFLSAPVGGG GGAVSAAVAV DDVVLRGAAG LRQLRDLGMY
     YAVAGNGGAT AAPPAPEPAA YRSLLGDGAA AEEMALFSSV KCTASAIDAP PKLDVHGVKS
     DKYRFACTNF DISGVQMGFR VVLRKSAMVG VFRRGGVTMV AVACAAAAAA TVACVLMARA
     LRRAVAREAA LGADLARHRD ALRQAERKSM NKSNAFASAS HDIRSALAAV AGLVEVSRPE
     ANPNIVDNLN QMELCTNKLL DILNSILDTT KVESGKVQLE EVEFNMADVL EESVDMANVV
     GITKGIEVIW DPCDFSVMKC DNIIGDSKRF KQILDNLLGN AMKFTQEGHV ILRAWANRPI
     ARGSIGAPSR FAYRSLENNF FSFFFGAKED RVSQNSFNPL QNDPNSVEFY FEVVDTGIGI
     PKEKRESVFE NYVQVKEGHG GTGLGLGIVQ SFVRLMGGEI SIKEKEPGER GTCFGFNVLL
     KTSGNQAAEE DIEEGPSTVS ELDIRASVFR ETNCFKGWHC ILFVHGDETR RVLQAWMESI
     GMKVWMVPGV ESISSTLEKA RSSRDDCDVD RCFSSKEMVS QVLPTTLRNN NIMARNLGEH
     HPLGMLLIVD VSNGQLENIQ RQARDFTQMR SQVPCKFVCL TDLRTSYKDF RRFEEMSCDL
     ILRKPVHGSR LYSLLMTLRD VQSSPMHRSS LVGHENYVTR HQDSANIVAL AEVGRLDQGL
     KTEEDRPLDG MHVLLVEDTL VLQTIQRKML NQLGAIVELA GDGAKAVDMF RDAIERASVS
     EEHSVPLPYD VIFMDCQMPR MDGYEATRRI REEESRYGIR TPIIALTAHS MEDDLQKAID
     VGMDLHMTKP IERRRIVEAV HGVCKGKN