OHK2_ORYSJ
ID OHK2_ORYSJ Reviewed; 1048 AA.
AC Q5SML4;
DT 16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable histidine kinase 2 {ECO:0000305};
DE Short=OsHK2 {ECO:0000303|PubMed:16891544};
DE EC=2.7.13.3 {ECO:0000305};
GN Name=HK2 {ECO:0000303|PubMed:17284581};
GN Synonyms=OHK1 {ECO:0000303|PubMed:16919402};
GN OrderedLocusNames=Os06g0183200 {ECO:0000312|EMBL:AP008212},
GN LOC_Os06g08450 {ECO:0000305};
GN ORFNames=OSJNBb0036B04.16 {ECO:0000312|EMBL:BAD72542.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16919402; DOI=10.1016/j.gene.2006.06.020;
RA Ito Y., Kurata N.;
RT "Identification and characterization of cytokinin-signalling gene families
RT in rice.";
RL Gene 382:57-65(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=16891544; DOI=10.1104/pp.106.086371;
RA Pareek A., Singh A., Kumar M., Kushwaha H.R., Lynn A.M.,
RA Singla-Pareek S.L.;
RT "Whole-genome analysis of Oryza sativa reveals similar architecture of two-
RT component signaling machinery with Arabidopsis.";
RL Plant Physiol. 142:380-397(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17284581; DOI=10.1104/pp.106.093666;
RA Schaller G.E., Doi K., Hwang I., Kieber J.J., Khurana J.P., Kurata N.,
RA Mizuno T., Pareek A., Shiu S.H., Wu P., Yip W.K.;
RT "Nomenclature for two-component signaling elements of rice.";
RL Plant Physiol. 143:555-557(2007).
CC -!- FUNCTION: Cytokinin receptor related to bacterial two-component
CC regulators. Functions as a histidine kinase and transmits the stress
CC signal to a downstream MAPK cascade. {ECO:0000250|UniProtKB:A1A698}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf blades, leaf sheaths,
CC shoot apex, flowers and panicles. {ECO:0000269|PubMed:16919402}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer. {ECO:0000305}.
CC -!- PTM: Activation probably requires a transfer of a phosphate group
CC between a His in the transmitter domain and an Asp of the receiver
CC domain. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Sterility. {ECO:0000269|PubMed:16891544}.
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DR EMBL; BR000243; FAA00247.1; -; Genomic_DNA.
DR EMBL; AP007226; BAD72542.1; -; Genomic_DNA.
DR EMBL; AP008212; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q5SML4; -.
DR SMR; Q5SML4; -.
DR STRING; 4530.OS06T0183200-00; -.
DR PaxDb; Q5SML4; -.
DR PRIDE; Q5SML4; -.
DR EnsemblPlants; Os06t0183200-01; Os06t0183200-01; Os06g0183200.
DR Gramene; Os06t0183200-01; Os06t0183200-01; Os06g0183200.
DR eggNOG; KOG0519; Eukaryota.
DR HOGENOM; CLU_000445_104_16_1; -.
DR InParanoid; Q5SML4; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0009927; F:histidine phosphotransfer kinase activity; IBA:GO_Central.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IBA:GO_Central.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0046777; P:protein autophosphorylation; IBA:GO_Central.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytokinin signaling pathway; Kinase; Membrane;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..1048
FT /note="Probable histidine kinase 2"
FT /id="PRO_0000433805"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..336
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..1048
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 398..662
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT DOMAIN 912..1044
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 401
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 975
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 1048 AA; 114674 MW; 85A65F6B8D21DE85 CRC64;
MREVEEVSKW RRRCCYFWIL FPLAVIATCM TITVVTFCSS TMYMTEVMGE ATKGAMDSAL
MHIAGNMRPL LEANRSVFTI ANTLHVQGNM ASFSHVGPKL FLAFSMQPLQ AQISYAAVDG
AAFAYYRAGG GDGEARAMFA RPNGTWFTQA VDPATGRPVG NATAAAPHQQ LPPNVTRLLL
DGGGGGASLA DGWARPGVRM LFLSAPVGGG GGAVSAAVAV DDVVLRGAAG LRQLRDLGMY
YAVAGNGGAT AAPPAPEPAA YRSLLGDGAA AEEMALFSSV KCTASAIDAP PKLDVHGVKS
DKYRFACTNF DISGVQMGFR VVLRKSAMVG VFRRGGVTMV AVACAAAAAA TVACVLMARA
LRRAVAREAA LGADLARHRD ALRQAERKSM NKSNAFASAS HDIRSALAAV AGLVEVSRPE
ANPNIVDNLN QMELCTNKLL DILNSILDTT KVESGKVQLE EVEFNMADVL EESVDMANVV
GITKGIEVIW DPCDFSVMKC DNIIGDSKRF KQILDNLLGN AMKFTQEGHV ILRAWANRPI
ARGSIGAPSR FAYRSLENNF FSFFFGAKED RVSQNSFNPL QNDPNSVEFY FEVVDTGIGI
PKEKRESVFE NYVQVKEGHG GTGLGLGIVQ SFVRLMGGEI SIKEKEPGER GTCFGFNVLL
KTSGNQAAEE DIEEGPSTVS ELDIRASVFR ETNCFKGWHC ILFVHGDETR RVLQAWMESI
GMKVWMVPGV ESISSTLEKA RSSRDDCDVD RCFSSKEMVS QVLPTTLRNN NIMARNLGEH
HPLGMLLIVD VSNGQLENIQ RQARDFTQMR SQVPCKFVCL TDLRTSYKDF RRFEEMSCDL
ILRKPVHGSR LYSLLMTLRD VQSSPMHRSS LVGHENYVTR HQDSANIVAL AEVGRLDQGL
KTEEDRPLDG MHVLLVEDTL VLQTIQRKML NQLGAIVELA GDGAKAVDMF RDAIERASVS
EEHSVPLPYD VIFMDCQMPR MDGYEATRRI REEESRYGIR TPIIALTAHS MEDDLQKAID
VGMDLHMTKP IERRRIVEAV HGVCKGKN
