OHK4_ORYSJ
ID OHK4_ORYSJ Reviewed; 1005 AA.
AC A1A698; A0A0P0W2A8; Q0DP41; Q10DW1;
DT 16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Probable histidine kinase 4 {ECO:0000305};
DE Short=OsHK4 {ECO:0000303|PubMed:22642989};
DE EC=2.7.13.3 {ECO:0000305};
DE AltName: Full=OsCRL1b {ECO:0000305};
GN Name=HK4 {ECO:0000303|PubMed:17284581};
GN Synonyms=OHK4 {ECO:0000303|PubMed:16919402};
GN OrderedLocusNames=Os03g0717700 {ECO:0000312|EMBL:BAF12997.2},
GN LOC_Os03g50860 {ECO:0000312|EMBL:ABF98563.1};
GN ORFNames=OsJ_12359 {ECO:0000312|EMBL:EEE59816.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16919402; DOI=10.1016/j.gene.2006.06.020;
RA Ito Y., Kurata N.;
RT "Identification and characterization of cytokinin-signalling gene families
RT in rice.";
RL Gene 382:57-65(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=16891544; DOI=10.1104/pp.106.086371;
RA Pareek A., Singh A., Kumar M., Kushwaha H.R., Lynn A.M.,
RA Singla-Pareek S.L.;
RT "Whole-genome analysis of Oryza sativa reveals similar architecture of two-
RT component signaling machinery with Arabidopsis.";
RL Plant Physiol. 142:380-397(2006).
RN [8]
RP INDUCTION BY CYTOKININ.
RX PubMed=17408920; DOI=10.1016/j.ygeno.2007.02.001;
RA Du L., Jiao F., Chu J., Jin G., Chen M., Wu P.;
RT "The two-component signal system in rice (Oryza sativa L.): a genome-wide
RT study of cytokinin signal perception and transduction.";
RL Genomics 89:697-707(2007).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17284581; DOI=10.1104/pp.106.093666;
RA Schaller G.E., Doi K., Hwang I., Kieber J.J., Khurana J.P., Kurata N.,
RA Mizuno T., Pareek A., Shiu S.H., Wu P., Yip W.K.;
RT "Nomenclature for two-component signaling elements of rice.";
RL Plant Physiol. 143:555-557(2007).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=22642989; DOI=10.1093/pcp/pcs079;
RA Choi J., Lee J., Kim K., Cho M., Ryu H., An G., Hwang I.;
RT "Functional identification of OsHk6 as a homotypic cytokinin receptor in
RT rice with preferential affinity for iP.";
RL Plant Cell Physiol. 53:1334-1343(2012).
CC -!- FUNCTION: Cytokinin receptor related to bacterial two-component
CC regulators. Functions as a histidine kinase and transmits the stress
CC signal to a downstream MAPK cascade. {ECO:0000269|PubMed:22642989}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in young leaves and spikelets, and
CC at lower levels in roots, mature leaves and stems.
CC {ECO:0000269|PubMed:22642989}.
CC -!- INDUCTION: By isopentenyladenine (iP), cis-zeatin (cZ) and
CC dihydrozeatin (DHZ) (PubMed:22642989). Induced by cytokinin in roots
CC (PubMed:17408920). {ECO:0000269|PubMed:17408920,
CC ECO:0000269|PubMed:22642989}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer. {ECO:0000305}.
CC -!- PTM: Activation probably requires a transfer of a phosphate group
CC between a His in the transmitter domain and an Asp of the receiver
CC domain. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Semi-dwarf, chlorina, late heading and low
CC fertility phenotypes. {ECO:0000269|PubMed:16891544}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF98563.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF12997.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BR000246; FAA00250.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98563.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008209; BAF12997.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS86088.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE59816.1; -; Genomic_DNA.
DR RefSeq; XP_015630313.1; XM_015774827.1.
DR AlphaFoldDB; A1A698; -.
DR SMR; A1A698; -.
DR STRING; 4530.OS03T0717700-01; -.
DR PaxDb; A1A698; -.
DR PRIDE; A1A698; -.
DR EnsemblPlants; Os03t0717700-01; Os03t0717700-01; Os03g0717700.
DR GeneID; 4333916; -.
DR Gramene; Os03t0717700-01; Os03t0717700-01; Os03g0717700.
DR KEGG; osa:4333916; -.
DR eggNOG; KOG0519; Eukaryota.
DR HOGENOM; CLU_000445_16_2_1; -.
DR OMA; QMESKAN; -.
DR OrthoDB; 51398at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; A1A698; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019955; F:cytokine binding; IDA:UniProtKB.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IDA:UniProtKB.
DR GO; GO:0004673; F:protein histidine kinase activity; IDA:UniProtKB.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IDA:UniProtKB.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.450.350; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR006189; CHASE_dom.
DR InterPro; IPR042240; CHASE_sf.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF03924; CHASE; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM01079; CHASE; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50839; CHASE; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytokinin signaling pathway; Kinase; Membrane;
KW Phosphoprotein; Reference proteome; Repeat; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..1005
FT /note="Probable histidine kinase 4"
FT /id="PRO_0000433809"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..333
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..1005
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 110..321
FT /note="CHASE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00049"
FT DOMAIN 389..675
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT DOMAIN 700..829
FT /note="Response regulatory 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 862..999
FT /note="Response regulatory 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 392
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 912
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 1005 AA; 109977 MW; 0D83685C93CCFC8E CRC64;
MGVGGGGGGG GGEAAAAVAV EGDEAGKGRR WWRVKVKLST VAVVAWVLAS AALWAGLHWR
FRRAALHKAE EALVCMCEER ARMLQDQFAV SVNHVHALAI LVATFHYDKH PPALDQDTFA
VYAARTSFER PLLSGVAYAQ RVVHADRESF ERQQGWIIKT MKHEPSPAQD EYAPVIYSQE
TISYIEGLDV MSGEEDRENI LRARATGKAV LTRPFRLMSN HLGVVLTFPV YLVDLPNDTA
VEDRVAATAG YLGGAFDVES LVENLLRQLA GNQELVVNVY DVTNHSNPLV MYGSEVPLGI
PSPSHTYTLD FGDPLRKHQM VCRYRNKLHV SWSAITTPSG VFVICMLVGY IIYAAWSRYD
NVKEDCRKME ALKKRAEAAD IAKSQFLATV SHEIRTPMNG VLGMLDMLLD TELKSTQRDY
AQTAQVCGKA LISLINEVLD RAKIEAGKID LESVPFDLRS ILDDVISLFS SKSREKGIEL
AVYVSERVPE ILLGDPGRFR QIITNLVGNS IKFTERGHIF VQVHLADHSN LATEAKIEPV
VNGMNGHKDE AIAIPTSGSH NTLSGFEAAD SRNNWENFKL LLSYEKNEMP YESDSDKVTL
VVSVEDTGIG IPLHAQGRVF TPFMQADSST SRNYGGTGIG LSISKCLVEI MGGQINFVSR
PLVGSTFTFT AVLRRCDKNA ISDSKTVALH PLPSSFKGLS ALLVDKRPVR ATVTKYHLQR
LGITSEVVGT IDPTFGVLSG RNGSSLTSIG KKQPCMLLIE SDSWGPQMDV SLHARLQEMK
QSDRIHVLPK VFLLSAAESD KVKKIHAVDS VIPKPLKASA LAACLFQALG ITQPSHEKRD
DSGSLHGRDG SGSLHGLLLG KNILVVDDNK VNLRVAAGTL KKYGAKVECV ESGKDALSLL
QVPHKFDLCL MDIQMPEMDG FEATRQIRAM EGKANEQADD SESGSEIAAK TAKWHLPILA
MTADVIQATH EECTKCGMDG YVSKPFEEKQ LFQAVQKFLG PCVSS
