OHK6_ORYSJ
ID OHK6_ORYSJ Reviewed; 970 AA.
AC A1A699; A0A0P0VP61; Q6Z5P3;
DT 16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Probable histidine kinase 6 {ECO:0000305};
DE Short=OsHK6 {ECO:0000303|PubMed:22642989};
DE EC=2.7.13.3 {ECO:0000305};
DE AltName: Full=OsCRL1a {ECO:0000305};
GN Name=HK6 {ECO:0000303|PubMed:17284581};
GN Synonyms=OHK5 {ECO:0000303|PubMed:16919402};
GN OrderedLocusNames=Os02g0738400 {ECO:0000312|EMBL:AP008208},
GN LOC_Os02g50480 {ECO:0000305};
GN ORFNames=P0684F11.6 {ECO:0000312|EMBL:BAD16039.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16919402; DOI=10.1016/j.gene.2006.06.020;
RA Ito Y., Kurata N.;
RT "Identification and characterization of cytokinin-signalling gene families
RT in rice.";
RL Gene 382:57-65(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP INDUCTION BY CYTOKININ.
RX PubMed=17408920; DOI=10.1016/j.ygeno.2007.02.001;
RA Du L., Jiao F., Chu J., Jin G., Chen M., Wu P.;
RT "The two-component signal system in rice (Oryza sativa L.): a genome-wide
RT study of cytokinin signal perception and transduction.";
RL Genomics 89:697-707(2007).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17284581; DOI=10.1104/pp.106.093666;
RA Schaller G.E., Doi K., Hwang I., Kieber J.J., Khurana J.P., Kurata N.,
RA Mizuno T., Pareek A., Shiu S.H., Wu P., Yip W.K.;
RT "Nomenclature for two-component signaling elements of rice.";
RL Plant Physiol. 143:555-557(2007).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND MUTAGENESIS OF HIS-365;
RP GLY-614 AND ASP-877.
RX PubMed=22642989; DOI=10.1093/pcp/pcs079;
RA Choi J., Lee J., Kim K., Cho M., Ryu H., An G., Hwang I.;
RT "Functional identification of OsHk6 as a homotypic cytokinin receptor in
RT rice with preferential affinity for iP.";
RL Plant Cell Physiol. 53:1334-1343(2012).
CC -!- FUNCTION: Cytokinin receptor related to bacterial two-component
CC regulators. Functions as a histidine kinase and transmits the stress
CC signal to a downstream MAPK cascade. {ECO:0000269|PubMed:22642989}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in spikelets and at lower levels
CC in roots, young leaves, mature leaves and stems.
CC {ECO:0000269|PubMed:22642989}.
CC -!- INDUCTION: Highly induced by isopentenyladenine (iP). Induced by cis-
CC zeatin (cZ) and dihydrozeatin (DHZ) (PubMed:22642989). Induced by
CC cytokinin in roots (PubMed:17408920). {ECO:0000269|PubMed:17408920,
CC ECO:0000269|PubMed:22642989}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer. {ECO:0000305}.
CC -!- PTM: Activation probably requires a transfer of a phosphate group
CC between a His in the transmitter domain and an Asp of the receiver
CC domain. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD16039.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BR000247; FAA00251.1; -; Genomic_DNA.
DR EMBL; AP005112; BAD16039.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008208; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP014958; BAS80826.1; -; Genomic_DNA.
DR RefSeq; XP_015626816.1; XM_015771330.1.
DR AlphaFoldDB; A1A699; -.
DR SMR; A1A699; -.
DR STRING; 4530.OS02T0738400-01; -.
DR PaxDb; A1A699; -.
DR PRIDE; A1A699; -.
DR EnsemblPlants; Os02t0738400-02; Os02t0738400-02; Os02g0738400.
DR GeneID; 107275680; -.
DR Gramene; Os02t0738400-02; Os02t0738400-02; Os02g0738400.
DR KEGG; osa:107275680; -.
DR eggNOG; KOG0519; Eukaryota.
DR HOGENOM; CLU_000445_16_2_1; -.
DR OrthoDB; 51398at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; A1A699; baseline and differential.
DR Genevisible; A1A699; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019955; F:cytokine binding; IDA:UniProtKB.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IDA:UniProtKB.
DR GO; GO:0004673; F:protein histidine kinase activity; IDA:UniProtKB.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IDA:UniProtKB.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.450.350; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR006189; CHASE_dom.
DR InterPro; IPR042240; CHASE_sf.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF03924; CHASE; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM01079; CHASE; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF52172; SSF52172; 2.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50839; CHASE; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Cytokinin signaling pathway; Kinase; Membrane;
KW Phosphoprotein; Reference proteome; Repeat; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..970
FT /note="Probable histidine kinase 6"
FT /id="PRO_0000433811"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..306
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..970
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 82..294
FT /note="CHASE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00049"
FT DOMAIN 362..651
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT DOMAIN 676..802
FT /note="Response regulatory 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 827..962
FT /note="Response regulatory 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 365
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 877
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MUTAGEN 365
FT /note="H->Q: Loss of function; when associated with A-614
FT and N-877."
FT /evidence="ECO:0000269|PubMed:22642989"
FT MUTAGEN 614
FT /note="G->A: Loss of function; when associated with Q-365
FT and N-877."
FT /evidence="ECO:0000269|PubMed:22642989"
FT MUTAGEN 877
FT /note="D->N: Loss of function; when associated with Q-365
FT and A-614."
FT /evidence="ECO:0000269|PubMed:22642989"
SQ SEQUENCE 970 AA; 108209 MW; FB0A1F1F992F72CA CRC64;
MGKPEARSGW RNAAAAAWVL VAVACAAYMH WHLRRETMDR AEERLVSMCE ERARMLQEQF
GVTVNHVHAL AILISTFHFE KFPSAIDQDT FAKYTARTSF ERPLLNGVAY AQRIFHHERE
MFENQQGWIM KTMKRQAAPP QDEYAPVIFS QDTVSYLARI DMMSGEEDRE NILRARATGK
AVLTNPFRLL GSNHLGVVLT FAVYRPGLAA DASVEERVEA TAGYLGGAFD VESLVENLLS
KLAGNQDIVV NVYDVTNASE PMAMYGPQSP DGKVSLFHVS TLDFGDPFRA HEMRCRYRQK
PPLPWSAITN PLGTFVIWML VGYIICAAWS RYDKVSEDCR KMEELKTQAE AADVAKSQFL
ATVSHEIRTP MNGVLGMLDM LLGTDLSMTQ KDYAQTAQMC GRALITLIND VLDRAKIEAG
KLELEAVPFD LRSLMDDVIS LFSSKSREKC IELAVFVCDD VPKVVIGDPW RYRQILTNLV
GNAVKFTERG HVFVRVCLAE NSKVEANQVL NGTMNGKDGK VETTANGAFN TLSGFQAADE
RNNWDYFKLL LSDKEPHMDE LECDRSYQND CDCVTLMISI EDTGVGIPLH AQDRVFTPFM
QADSSTSRNY GGTGIGLSIS KCLAELMGGQ ISFTSRPFVG STFTFSAVLK RSCKDTSSDS
KRSLSEALPT AFKGMKAILV DGRPVRGAVT RYHLNRLGIV VKVVNNLSMG LQTLAGQNGV
KESREKLSML FIESDIWRPE TDILLLNRLH ELKNNGQVHE LPKLVLLVTS EADKDRYGSA
FDIVMYKPIR ASTIASCLQQ LLKVVMPERK DNQNRPSFLR SLLIGKNILI VDDNKVNLRV
AAAALKKYGA KVHCVESGKD AVSLLQQPHC FDACFMDVQM PEMDGFEATR QIRQMEVKAN
EERKALDLME GSTFVESHLP VLAMTADVIQ ATYEECIKSG MDGYVSKPFD EEQLYQAVSR
LVVGTKESAV
