OIT3_HUMAN
ID OIT3_HUMAN Reviewed; 545 AA.
AC Q8WWZ8; A0AVP3; Q8N1M8;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Oncoprotein-induced transcript 3 protein;
DE AltName: Full=Liver-specific zona pellucida domain-containing protein;
DE Flags: Precursor;
GN Name=OIT3; Synonyms=LZP; ORFNames=UNQ826/PRO1753;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Liver;
RX PubMed=12939600; DOI=10.1053/jhep.2003.50340;
RA Xu Z.-G., Du J.-J., Zhang X., Cheng Z.-H., Ma Z.-Z., Xiao H.-S., Yu L.,
RA Wang Z.-Q., Li Y.-Y., Huo K.-K., Han Z.-G.;
RT "A novel liver-specific zona pellucida domain containing protein that is
RT expressed rarely in hepatocellular carcinoma.";
RL Hepatology 38:735-744(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-291.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
CC -!- FUNCTION: May be involved in hepatocellular function and development.
CC {ECO:0000269|PubMed:12939600}.
CC -!- INTERACTION:
CC Q8WWZ8; Q9UGL9: CRCT1; NbExp=3; IntAct=EBI-10277776, EBI-713677;
CC Q8WWZ8; Q9NWN3: FBXO34; NbExp=3; IntAct=EBI-10277776, EBI-719816;
CC Q8WWZ8; P49639: HOXA1; NbExp=5; IntAct=EBI-10277776, EBI-740785;
CC Q8WWZ8; Q8IUC1: KRTAP11-1; NbExp=3; IntAct=EBI-10277776, EBI-1052037;
CC Q8WWZ8; Q5T751: LCE1C; NbExp=3; IntAct=EBI-10277776, EBI-12224199;
CC Q8WWZ8; Q5T754: LCE1F; NbExp=3; IntAct=EBI-10277776, EBI-11958008;
CC Q8WWZ8; Q5TA76: LCE3A; NbExp=3; IntAct=EBI-10277776, EBI-9394625;
CC Q8WWZ8; P50222: MEOX2; NbExp=3; IntAct=EBI-10277776, EBI-748397;
CC Q8WWZ8; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-10277776, EBI-16439278;
CC Q8WWZ8; P32242: OTX1; NbExp=3; IntAct=EBI-10277776, EBI-740446;
CC Q8WWZ8; Q08AM6: VAC14; NbExp=3; IntAct=EBI-10277776, EBI-2107455;
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:12939600}.
CC Note=Can be secreted into blood.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8WWZ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8WWZ8-2; Sequence=VSP_027482, VSP_027483;
CC -!- TISSUE SPECIFICITY: Liver-specific. Expressed only in the hepatocytes.
CC Down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines.
CC {ECO:0000269|PubMed:12939600}.
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DR EMBL; AY013707; AAG40096.2; -; mRNA.
DR EMBL; AY358339; AAQ88705.1; -; mRNA.
DR EMBL; AK096435; BAC04788.1; -; mRNA.
DR EMBL; BC126440; AAI26441.1; -; mRNA.
DR CCDS; CCDS7318.1; -. [Q8WWZ8-1]
DR RefSeq; NP_689848.1; NM_152635.2. [Q8WWZ8-1]
DR AlphaFoldDB; Q8WWZ8; -.
DR SMR; Q8WWZ8; -.
DR BioGRID; 128002; 73.
DR IntAct; Q8WWZ8; 13.
DR MINT; Q8WWZ8; -.
DR STRING; 9606.ENSP00000333900; -.
DR GlyConnect; 1964; 7 N-Linked glycans (5 sites).
DR GlyGen; Q8WWZ8; 6 sites, 8 N-linked glycans (5 sites).
DR iPTMnet; Q8WWZ8; -.
DR PhosphoSitePlus; Q8WWZ8; -.
DR BioMuta; OIT3; -.
DR DMDM; 74730985; -.
DR MassIVE; Q8WWZ8; -.
DR PaxDb; Q8WWZ8; -.
DR PeptideAtlas; Q8WWZ8; -.
DR PRIDE; Q8WWZ8; -.
DR ProteomicsDB; 74973; -. [Q8WWZ8-1]
DR ProteomicsDB; 74974; -. [Q8WWZ8-2]
DR Antibodypedia; 29281; 114 antibodies from 19 providers.
DR DNASU; 170392; -.
DR Ensembl; ENST00000334011.10; ENSP00000333900.5; ENSG00000138315.13. [Q8WWZ8-1]
DR Ensembl; ENST00000622652.1; ENSP00000479787.1; ENSG00000138315.13. [Q8WWZ8-2]
DR GeneID; 170392; -.
DR KEGG; hsa:170392; -.
DR MANE-Select; ENST00000334011.10; ENSP00000333900.5; NM_152635.3; NP_689848.1.
DR UCSC; uc001jte.2; human. [Q8WWZ8-1]
DR CTD; 170392; -.
DR DisGeNET; 170392; -.
DR GeneCards; OIT3; -.
DR HGNC; HGNC:29953; OIT3.
DR HPA; ENSG00000138315; Tissue enriched (liver).
DR MIM; 609330; gene.
DR neXtProt; NX_Q8WWZ8; -.
DR OpenTargets; ENSG00000138315; -.
DR PharmGKB; PA142671231; -.
DR VEuPathDB; HostDB:ENSG00000138315; -.
DR eggNOG; ENOG502QW18; Eukaryota.
DR GeneTree; ENSGT00940000157851; -.
DR HOGENOM; CLU_037129_2_0_1; -.
DR InParanoid; Q8WWZ8; -.
DR OMA; GPQSQML; -.
DR OrthoDB; 319810at2759; -.
DR PhylomeDB; Q8WWZ8; -.
DR TreeFam; TF330284; -.
DR PathwayCommons; Q8WWZ8; -.
DR SignaLink; Q8WWZ8; -.
DR BioGRID-ORCS; 170392; 8 hits in 1065 CRISPR screens.
DR ChiTaRS; OIT3; human.
DR GenomeRNAi; 170392; -.
DR Pharos; Q8WWZ8; Tbio.
DR PRO; PR:Q8WWZ8; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q8WWZ8; protein.
DR Bgee; ENSG00000138315; Expressed in liver and 56 other tissues.
DR Genevisible; Q8WWZ8; HS.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005201; F:extracellular matrix structural constituent; IBA:GO_Central.
DR GO; GO:1903118; P:urate homeostasis; IEA:Ensembl.
DR Gene3D; 2.60.40.4100; -; 1.
DR InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR018097; EGF_Ca-bd_CS.
DR InterPro; IPR042235; ZP-C.
DR InterPro; IPR001507; ZP_dom.
DR Pfam; PF00100; Zona_pellucida; 1.
DR PRINTS; PR00023; ZPELLUCIDA.
DR SMART; SM00181; EGF; 3.
DR SMART; SM00179; EGF_CA; 2.
DR SMART; SM00241; ZP; 1.
DR PROSITE; PS00010; ASX_HYDROXYL; 1.
DR PROSITE; PS01187; EGF_CA; 1.
DR PROSITE; PS51034; ZP_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Disulfide bond; EGF-like domain;
KW Glycoprotein; Nucleus; Reference proteome; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..545
FT /note="Oncoprotein-induced transcript 3 protein"
FT /id="PRO_0000298931"
FT DOMAIN 182..222
FT /note="EGF-like; calcium-binding"
FT /evidence="ECO:0000255"
FT DOMAIN 261..516
FT /note="ZP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00375"
FT REGION 518..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218"
FT CARBOHYD 299
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 186..197
FT /evidence="ECO:0000250"
FT DISULFID 193..206
FT /evidence="ECO:0000250"
FT DISULFID 208..221
FT /evidence="ECO:0000250"
FT VAR_SEQ 318..321
FT /note="VVND -> LCFR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027482"
FT VAR_SEQ 322..545
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027483"
FT VARIANT 237
FT /note="S -> P (in dbSNP:rs35089256)"
FT /id="VAR_034742"
SQ SEQUENCE 545 AA; 60022 MW; E4BDE126213484B0 CRC64;
MPPFLLLTCL FITGTSVSPV ALDPCSAYIS LNEPWRNTDH QLDESQGPPL CDNHVNGEWY
HFTGMAGDAM PTFCIPENHC GTHAPVWLNG SHPLEGDGIV QRQACASFNG NCCLWNTTVE
VKACPGGYYV YRLTKPSVCF HVYCGHFYDI CDEDCHGSCS DTSECTCAPG TVLGPDRQTC
FDENECEQNN GGCSEICVNL KNSYRCECGV GRVLRSDGKT CEDVEGCHNN NGGCSHSCLG
SEKGYQCECP RGLVLSEDNH TCQVPVLCKS NAIEVNIPRE LVGGLELFLT NTSCRGVSNG
THVNILFSLK TCGTVVDVVN DKIVASNLVT GLPKQTPGSS GDFIIRTSKL LIPVTCEFPR
LYTISEGYVP NLRNSPLEIM SRNHGIFPFT LEIFKDNEFE EPYREALPTL KLRDSLYFGI
EPVVHVSGLE SLVESCFATP TSKIDEVLKY YLIRDGCVSD DSVKQYTSRD HLAKHFQVPV
FKFVGKDHKE VFLHCRVLVC GVLDERSRCA QGCHRRMRRG AGGEDSAGLQ GQTLTGGPIR
IDWED
