OL56_STRAT
ID OL56_STRAT Reviewed; 3519 AA.
AC Q07017;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Oleandomycin polyketide synthase, modules 5 and 6;
GN Name=orfB;
OS Streptomyces antibioticus.
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=1890;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8107683; DOI=10.1007/bf00280426;
RA Swan D.G., Rodriguez A.M., Vilches C., Mendez C., Salas J.A.;
RT "Characterisation of a Streptomyces antibioticus gene encoding a type I
RT polyketide synthase which has an unusual coding sequence.";
RL Mol. Gen. Genet. 242:358-362(1994).
CC -!- FUNCTION: May be involved in the biosynthesis of the oleandomycin
CC lactone ring.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Note=Binds 2 phosphopantetheines covalently.;
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DR EMBL; L09654; AAA19695.1; -; Unassigned_DNA.
DR PIR; S43048; S43048.
DR SMR; Q07017; -.
DR ESTHER; strat-ol56; Thioesterase.
DR PRIDE; Q07017; -.
DR KEGG; ag:AAA19695; -.
DR BioCyc; MetaCyc:MON-17052; -.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR Gene3D; 1.10.1200.10; -; 2.
DR Gene3D; 3.40.366.10; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR020802; PKS_thioesterase.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR036299; Polyketide_synth_docking_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR001031; Thioesterase.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF18369; PKS_DE; 2.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF00975; Thioesterase; 1.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SMART; SM00824; PKS_TE; 1.
DR SUPFAM; SSF101173; SSF101173; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR SUPFAM; SSF51735; SSF51735; 4.
DR SUPFAM; SSF52151; SSF52151; 2.
DR SUPFAM; SSF53474; SSF53474; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR SUPFAM; SSF55048; SSF55048; 2.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 3: Inferred from homology;
KW Acyltransferase; Antibiotic biosynthesis; Multifunctional enzyme; NADP;
KW Phosphopantetheine; Phosphoprotein; Repeat; Transferase.
FT CHAIN 1..3519
FT /note="Oleandomycin polyketide synthase, modules 5 and 6"
FT /id="PRO_0000180297"
FT DOMAIN 1489..1564
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3143..3218
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 1..?
FT /note="Module 5"
FT REGION 32..501
FT /note="Beta-ketoacyl synthase 1"
FT REGION 569..890
FT /note="Acyltransferase (AT) 1"
FT REGION 1200..1382
FT /note="Beta-ketoacyl reductase 1"
FT REGION 1686..2156
FT /note="Beta-ketoacyl synthase 2"
FT REGION 2220..2541
FT /note="Acyltransferase (AT) 2"
FT REGION 2856..3038
FT /note="Beta-ketoacyl reductase 2"
FT REGION 3224..3245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3270..3519
FT /note="Thioesterase"
FT REGION ?..3519
FT /note="Module 6"
FT ACT_SITE 210
FT /note="For beta-ketoacyl synthase 1 activity"
FT ACT_SITE 660
FT /note="Acyl-ester intermediate"
FT ACT_SITE 1859
FT /note="For beta-ketoacyl synthase 2 activity"
FT ACT_SITE 2311
FT /note="Acyl-ester intermediate"
FT BINDING 1203..1249
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT BINDING 2859..2905
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT MOD_RES 1524
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3178
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 3519 AA; 368567 MW; 41AE78AAAEB61F86 CRC64;
MAEAEKLREY LWRATTELKE VSDRLRETEE RAREPIAIVG MSCRFPGGGD ATVNTPEQFW
DLLNSGGDGI AGLPEDRGWD LGRLYDPDPD RAGTSYVREG GFLYDSGEFD AAFFGISPRE
ALAMDPQQRL LLETSWEAFE SAGIKRAALR GSDTGVYIGA WSTGYAGSPY RLVEGLEGQL
AIGTTLGAAS GRVAYTFGLE GPAVTVDTAC SSSLVALHLA VQGLRRGECS LALVGGVTVM
SSPVTLTTFS RQRGLSVDGR CKAFPASADG FGAAEGVGVL LVERLSDARR LGHRVLAVVR
GSAVNQDGAS NGLTAPNGPS QQRVIRAALA DAGLAPADVD VVEAHGTGTR LGDPIEAQAL
LATYGQGRAG GRPVWLGSVK SNIGHTQAAA GVAGVMKMVL ALGRGVVPKT LHVDEPSPHV
DWSAGAVELL TEERPWEPEA ERLRRAGISA FGVSGTNAHV IVEEAPAEPE PEPGTRVVAA
GDLVVPWVVS GRDARALRAQ AARLAAHVSG VSAVDVGWSL VATRSVFEHR AVAIGSELDS
MAGSLAGFAA GGVVPGVVSG VAPAEGRRVV FVFPGQGSQW VGMAAGLLDA CPVFAEAVAE
CAAVLDPVTG WSLVEVLQGR DATVLGRVDV VQPALWAVMV SLARTWRYYG VEPAAVVGHS
QGEIAAACVA GGLSLADGAR VVVLRSRAIA RIAGGGGMVS VSLPAGRVRT MLEEFDGRLS
VAAVNGPSST VVSGDVQALD ELLAGCEREG VRARRVPVDY ASHSAQMDQL RDELLEALAD
ITPQDSSVPF FSTVTADWLG TTALGAGYWF TNLRETVRFQ EAVEGLVAQG MGAFVECSPH
PVLVPGIEQT LDALDQNAAV FGSLRRDEGG LDRFLTSLAE AFVQGVPVDW SRAFEGVTPR
TVDLPTYPFQ RQHYWLMAEE APVSQPPHSE NSFWSVVADA DAEAAAELLG VDVEAVEAVM
PALSSWHRQS QLRAEVNQWR YDVAWKRLTT GALPEKPGNW LVVTPAGTDT TFAESLARTA
AAELGVSVSF AQVDTAHPDR SQYAHALRQA LTGPENVDHL VSLLALDQAT DDLAAAPSCL
AASLVLAQAL VDLGRVGEGP RLWLVTRGAV VAGPSDAGAV IDPVQAQVWG FGRVLGLEHP
ELWGGLIDLP VGVDEEVCRR FVGVVASAGF EDQVAVRGSG VWVRRLVRAV VDGGGGGWRP
RGTVLVTGGL GGLGAHTARW LVGGGADHVV LVSRRGGSAP GAGDLVRELE GLGGARVSVR
ACDVADRVAL RALLSDLGEP VTAVFHAAGV PQSTPLAEIS VQEAADVMAA KVAGAVNLGE
LVDPCGLEAF VLFSSNAGVW GSGGQAVYAA ANAFLDALAV RRRGVGLPAT SVAWGMWAGE
GMASVGGAAR ELSRRGVRAM DPERAVAVMA DAVGRGEAFV AVADVDWERF VTGFASARPR
PLISDLPEVR AVVEGQVQGR GQGLGLVGEE ESSGWLKRLS GLSRVRQEEE LVELVRAQAA
VVLGHGSAQD VPAERAFKEL GFDSLTAVEL RNGLAAATGI RLPATMAFDH PNATAIARFL
QSQLLPDAES ESAVPSSPED EVRQALASLS LDQLKGAGLL DPLLALTRLR EINSTVQNPE
PTTESIDEMD GETCCAWRSA KSTAEPLTTG ADMPDPTAKY VEALRASLKE NERLRQQNHS
LLAASREAIA ITAMSCRFGG GIDSPEDLWR FLAEGRDAVA GLPEDRGWDL DALYHPDPEN
PGTTYVREGA FRYDAAQFDA GFFGISPREA LAMDPQQRLL LETSWELFER ADIDPYTVRG
TATGIFIGAG HQGYGPDPKR APESVAGYLL TGTASAVLSG RISYTFGLEG PAVTVDTACS
SSLVALHLAV QALRRGECSL AIAGGVAVMS TPDAFVEFSR QQGMARDGRC KAFAAAADGM
GWGEGVSLLL LERLSDARRL GHRVLAVVRG SAVNQDGASN GLAAPNGPSQ QRVIRAALAD
AGLAPADVDV VEAHGTGTRL GDPIEAQALL ATYGQGRAGG RPVWLGSVKS NIGHTQAAAG
VAGVMKMVLA LGRGVVPKTL HVDEPSPHVD WSAGAVELLT EERPWEPEAE RLRRAGISAF
GVSGTNAHVI VEEAPAEPEP EPGTRVVAAG DLVVPWVVSG RDVGALREQA ARLAAHVSST
GAGVVDVGWS LVATRSVFEH RAVMVGTDLD SMAGSLAGFA AGGVVPGVVS GVAPAEGRRV
VFVFPGQGSQ WVGMAAGLLD ACPVFAEAVA ECAAVLDPVT GWSLVEVLQG RDATVLGRVD
VVQPALWAVM VSLARTWRYY GVEPAAVVGH SQGEIAAACV AGGLSLADGA RVVVLRSRAI
ARIAGGGGMV SVSLPAGRVR TMLDTYGGRV SVAAVNGPSS TVVSGDVQAL DELLAGCERE
GVRARRVPVD YASHSAQMDQ LRDELLEALA DITPQDSSVP FFSTVTADWL DTTALDAGYW
FTNLRETVRF QEAVEGLVAQ GMGAFVECSP HPVLVPGIEQ TLDALDQNAA VLGSLRRDEG
GLDRLLTSLA EAFVQGVPVD WTHAFEGVTP RTVDLPTYPF QRQRFWLDGS PASSANGVDG
EADAMIWDAV EREDSVAVAE ELGIDAEALH TVLPALSSWR RRRVEHRRLQ DWRYRVEWKP
FPAALDEVLG GGWLFVVPRG LADDGVVARV VAAVTARGGE VSVVELDPTR PDRRAYAEAV
AGRGVSGVVS FLSWDDRRHS EHPVVPAGLA ASLVLAQALV DLGRVGEGPR LWLVTRDAVV
AGPSDAGAVI DPVQAQVWGF GRVLGLEHPE LWGGLIDLPV EAPEPGSTCD HTYADLLATV
VASAGFEDQV AVRGSGVWVR RLVRAVVDGG GGGWRPRGTV LVTGGLGGLG AHTARWLVGG
GADHVVLVSR RGGSAPGAGD LVRELEGLGG ARVSVRACDV ADRVALRALL SDLGEPVTAV
FHAAGVPQST PLAEISVQEA ADVMAAKVAG AVNLGELVDP CGLEAFVLFS SNAGVWGSGG
QAVYAAANAF LDALAVRRRG VGLPATSVAW GMWAGEGMAS VGGAARELSR RGVRAMDPER
AVAVMADAVG RGEAFVAVAD VDWERFVTGF ASARPRPLIS DLPEVRTALR NQEQEQLHAP
VPEDRSAQLL RRLSMLSPAG REAELVKLVR TEAAAVLGHG SAQDVPAERA FKELGFDSLT
AVQLRNRLAA ATGTRLPASA VFDHPHAAAL ARWLLAGMRH ADGGHGGGHA GGPGPDADEG
RSAGAGHSGM LADLYRRSAE LGRSREFIGL LADTAAFRPV FHGPADLDAP LEAVPLADGV
RKPQLICCSG TAPVGGPHEF ARLASFFRGT RAVSALPLPG YLPGEQLPAD LDAVLAAQAE
AVEKQTGGAP FVLVGYSAGG LMAHALACHL AGRGTPPSGE VLVDVYPPGR QEPVFGWQKE
LTEGMFAQDF VPMDDTRLTA LGTYDRLMGE WRPAPSGLPT LLIRATEPMA EWTGAIDWRA
SWEYDHTAVD MPGNHFTIMR EHAEDAARHI DVWLKGLTP
