OL734_MOUSE
ID OL734_MOUSE Reviewed; 313 AA.
AC Q8VFT4;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Olfactory receptor 734 {ECO:0000303|PubMed:31230984};
GN Name=Olfr734 {ECO:0000303|PubMed:31230984, ECO:0000312|MGI:MGI:3030568};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11875048; DOI=10.1093/hmg/11.5.535;
RA Young J.M., Friedman C., Williams E.M., Ross J.A., Tonnes-Priddy L.,
RA Trask B.J.;
RT "Different evolutionary processes shaped the mouse and human olfactory
RT receptor gene families.";
RL Hum. Mol. Genet. 11:535-546(2002).
RN [2]
RP ERRATUM OF PUBMED:11875048.
RA Young J.M., Friedman C., Williams E.M., Ross J.A., Tonnes-Priddy L.,
RA Trask B.J.;
RL Hum. Mol. Genet. 11:1683-1683(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11802173; DOI=10.1038/nn800;
RA Zhang X., Firestein S.;
RT "The olfactory receptor gene superfamily of the mouse.";
RL Nat. Neurosci. 5:124-133(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14611657; DOI=10.1186/gb-2003-4-11-r71;
RA Young J.M., Shykind B.M., Lane R.P., Tonnes-Priddy L., Ross J.A.,
RA Walker M., Williams E.M., Trask B.J.;
RT "Odorant receptor expressed sequence tags demonstrate olfactory expression
RT of over 400 genes, extensive alternate splicing and unequal expression
RT levels.";
RL Genome Biol. 4:R71.1-R71.13(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain {ECO:0000312|EMBL:AAI41030.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=31798959; DOI=10.1038/s41421-019-0122-x;
RA Wei F., Long A., Wang Y.;
RT "The Asprosin-OLFR734 hormonal signaling axis modulates male fertility.";
RL Cell Discov. 5:55-55(2019).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF GLU-173 AND ARG-186.
RX PubMed=31230984; DOI=10.1016/j.cmet.2019.05.022;
RA Li E., Shan H., Chen L., Long A., Zhang Y., Liu Y., Jia L., Wei F., Han J.,
RA Li T., Liu X., Deng H., Wang Y.;
RT "OLFR734 mediates glucose metabolism as a receptor of asprosin.";
RL Cell Metab. 30:319-328(2019).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32337066; DOI=10.1038/s41421-020-0152-4;
RA Liu Y., Long A., Chen L., Jia L., Wang Y.;
RT "The Asprosin-OLFR734 module regulates appetitive behaviors.";
RL Cell Discov. 6:19-19(2020).
CC -!- FUNCTION: Olfactory receptor that acts as a receptor of Asprosin
CC hormone at the surface of hepatocytes to promote hepatocyte glucose
CC release (PubMed:31230984). Also binds Asprosin in the arcuate nucleus
CC of the hypothalamus, thereby stimulating appetite by promoting
CC orexigenic AgRP neuronal activity (PubMed:32337066). In testis,
CC Asprosin-binding promotes sperm progressive motility and enhances male
CC fertility (PubMed:31798959). The activity of this receptor is mediated
CC by G proteins which activate adenylyl cyclase, resulting in an
CC elevation of intracellular cAMP (PubMed:31230984).
CC {ECO:0000269|PubMed:31230984, ECO:0000269|PubMed:31798959,
CC ECO:0000269|PubMed:32337066}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:31230984};
CC Multi-pass membrane protein {ECO:0000255|RuleBase:RU363047}.
CC -!- TISSUE SPECIFICITY: Highly expressed in liver but not in adipose tissue
CC (PubMed:31230984). Also expressed at high level in testis
CC (PubMed:31798959). {ECO:0000269|PubMed:31230984,
CC ECO:0000269|PubMed:31798959}.
CC -!- DISRUPTION PHENOTYPE: Compared to wild-type mice, mutant mice fed on a
CC regular diet show a remarkable decrease in body weight, food intake,
CC fat mass and plasma lipids (PubMed:31230984). Blunted response to
CC Asprosin, including attenuated cAMP levels and hepatic glucose
CC production, as well as improved insulin sensitivity (PubMed:31230984).
CC Mutant mice show significantly reduced food intake in overnight-fasted
CC mice compared with wild-type mice (PubMed:32337066). Male mice display
CC decreased fertility caused by impaired sperm motility
CC (PubMed:31798959). {ECO:0000269|PubMed:31230984,
CC ECO:0000269|PubMed:31798959, ECO:0000269|PubMed:32337066}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000305}.
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DR EMBL; AY073432; AAL61095.1; -; Genomic_DNA.
DR EMBL; AY317859; AAP71197.1; -; Genomic_DNA.
DR EMBL; BC141029; AAI41030.1; -; mRNA.
DR CCDS; CCDS27010.1; -.
DR RefSeq; NP_666875.1; NM_146664.1.
DR AlphaFoldDB; Q8VFT4; -.
DR SMR; Q8VFT4; -.
DR STRING; 10090.ENSMUSP00000057376; -.
DR PaxDb; Q8VFT4; -.
DR PRIDE; Q8VFT4; -.
DR Ensembl; ENSMUST00000050928; ENSMUSP00000057376; ENSMUSG00000045306.
DR Ensembl; ENSMUST00000217152; ENSMUSP00000150732; ENSMUSG00000045306.
DR GeneID; 258658; -.
DR KEGG; mmu:258658; -.
DR UCSC; uc007tkx.1; mouse.
DR CTD; 258658; -.
DR MGI; MGI:3030568; Olfr734.
DR VEuPathDB; HostDB:ENSMUSG00000045306; -.
DR eggNOG; ENOG502SKDS; Eukaryota.
DR GeneTree; ENSGT00940000163142; -.
DR HOGENOM; CLU_012526_8_1_1; -.
DR InParanoid; Q8VFT4; -.
DR OMA; MNRHLCC; -.
DR OrthoDB; 980902at2759; -.
DR PhylomeDB; Q8VFT4; -.
DR TreeFam; TF338273; -.
DR BioGRID-ORCS; 258658; 1 hit in 65 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8VFT4; protein.
DR Bgee; ENSMUSG00000045306; Expressed in respiratory tract epithelium and 4 other tissues.
DR GO; GO:0016021; C:integral component of membrane; ISA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISA:MGI.
DR GO; GO:0042593; P:glucose homeostasis; IDA:UniProtKB.
DR GO; GO:0032100; P:positive regulation of appetite; IMP:UniProtKB.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; IMP:UniProtKB.
DR GO; GO:1902093; P:positive regulation of flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0007608; P:sensory perception of smell; ISA:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000725; Olfact_rcpt.
DR Pfam; PF13853; 7tm_4; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00245; OLFACTORYR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Olfaction; Receptor; Reference proteome; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..313
FT /note="Olfactory receptor 734"
FT /id="PRO_0000453783"
FT TOPO_DOM 1..25
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 58..78
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..97
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 98..118
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 143..163
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..204
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 205..225
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 239..259
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..270
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 271..291
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..313
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 97..179
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT MUTAGEN 173
FT /note="E->A: Reduced affinity for Asprosin."
FT /evidence="ECO:0000269|PubMed:31230984"
FT MUTAGEN 186
FT /note="R->D: Reduced affinity for Asprosin."
FT /evidence="ECO:0000269|PubMed:31230984"
SQ SEQUENCE 313 AA; 35601 MW; 5A73BDE2898D6A5F CRC64;
MEPANDTTVT EFILTGLSQT REVQLVLFVI FLSFYLFILP VNILIICTIR LDSHLSSPMY
FLLANLAFLD IWYSSITAPK MLVDFFVERK IISFGGCIAQ LFFLHFVGAS EMFLLTVMAF
DRYAAICRPL HYATIMNRRL CCILVALSWT GGFVHSIIQV ALIVRLPFCG PNELDNYFCD
ITQVVRIACA NTFLEEMVMI FSSGLISVVC FIALLMSYAF LLTMLKKHSS SGESTSRAIS
TCYSHITIVV LMFGPSIYIY ARPFDSFSLD KVVSVFHTVI FPLLNPIIYT LRNKEVKAAM
RKLVNRYIFC KEK
