ID   OLEO1_SOYBN             Reviewed;         226 AA.
AC   P29530;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=P24 oleosin isoform A;
DE   AltName: Full=P89;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Century 84;
RX   PubMed=9084495; DOI=10.1016/s0005-2760(97)00004-0;
RA   Rowley D.L., Herman E.M.;
RT   "The upstream domain of soybean oleosin genes contains regulatory elements
RT   similar to those of legume storage proteins.";
RL   Biochim. Biophys. Acta 1345:1-4(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 75-226.
RC   STRAIN=cv. Century;
RX   PubMed=1932682; DOI=10.1007/bf00037150;
RA   Kalinski A., Loer D.S., Weisemann J.M., Matthews B.F., Herman E.M.;
RT   "Isoforms of soybean seed oil body membrane protein 24 kDa oleosin are
RT   encoded by closely related cDNAs.";
RL   Plant Mol. Biol. 17:1095-1098(1991).
CC   -!- FUNCTION: May have a structural role to stabilize the lipid body during
CC       desiccation of the seed by preventing coalescence of the oil. Probably
CC       interacts with both lipid and phospholipid moieties of lipid bodies.
CC       May also provide recognition signals for specific lipase anchorage in
CC       lipolysis during seedling growth.
CC   -!- SUBCELLULAR LOCATION: Lipid droplet. Membrane; Multi-pass membrane
CC       protein. Note=Surface of oil bodies. Oleosins exist at a monolayer
CC       lipid/water interface.
CC   -!- SIMILARITY: Belongs to the oleosin family. {ECO:0000305}.
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DR   EMBL; U09118; AAA17854.1; -; Unassigned_DNA.
DR   EMBL; X60772; CAA43182.1; -; mRNA.
DR   PIR; S17935; S17935.
DR   PIR; T06378; T06378.
DR   AlphaFoldDB; P29530; -.
DR   SMR; P29530; -.
DR   STRING; 3847.GLYMA19G13060.1; -.
DR   PRIDE; P29530; -.
DR   eggNOG; ENOG502S1R0; Eukaryota.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0012511; C:monolayer-surrounded lipid storage body; IBA:GO_Central.
DR   GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR   GO; GO:0050826; P:response to freezing; IBA:GO_Central.
DR   GO; GO:0010344; P:seed oilbody biogenesis; IBA:GO_Central.
DR   InterPro; IPR000136; Oleosin.
DR   PANTHER; PTHR33203; PTHR33203; 1.
DR   Pfam; PF01277; Oleosin; 1.
DR   PROSITE; PS00811; OLEOSINS; 1.
PE   2: Evidence at transcript level;
KW   Lipid droplet; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..226
FT                   /note="P24 oleosin isoform A"
FT                   /id="PRO_0000108142"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          159..165
FT                   /note="1-1"
FT   REPEAT          166..173
FT                   /note="1-2"
FT   REPEAT          184..191
FT                   /note="2-1"
FT   REPEAT          192..199
FT                   /note="2-2"
FT   REPEAT          200..207
FT                   /note="2-3"
FT   REGION          1..76
FT                   /note="Polar"
FT   REGION          77..130
FT                   /note="Hydrophobic"
FT   REGION          159..173
FT                   /note="2 X 7 AA tandem repeats"
FT   REGION          164..226
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          184..207
FT                   /note="3 X 8 AA tandem repeats"
FT   COMPBIAS        164..215
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        75..76
FT                   /note="LT -> NS (in Ref. 2; CAA43182)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   226 AA;  23502 MW;  D3AE88135654167B CRC64;
     MTTQVPPHSV QVHTTTTHRY EAGVVPPGAR FETSYEAGVK AASIYHSERG PTTSQVLAVL
     AGLPVGGILL LLAGLTLAGT LTGLAVATPL FVLFSPVLVP ATVAIGLAVA GFLTSGAFGL
     TALSSFSWIL NYIRETQPAS ENLAAAAKHH LAEAAEYVGQ KTKEVGQKTK EVGQDIQSKA
     QDTREAAARD AREAAARDAR EAAARDAKVE ARDVKRTTVT ATTATA