ID   OLEO2_SOYBN             Reviewed;         223 AA.
AC   P29531;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=P24 oleosin isoform B;
DE   AltName: Full=P91;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Century;
RX   PubMed=1932682; DOI=10.1007/bf00037150;
RA   Kalinski A., Loer D.S., Weisemann J.M., Matthews B.F., Herman E.M.;
RT   "Isoforms of soybean seed oil body membrane protein 24 kDa oleosin are
RT   encoded by closely related cDNAs.";
RL   Plant Mol. Biol. 17:1095-1098(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Century 84;
RX   PubMed=9084495; DOI=10.1016/s0005-2760(97)00004-0;
RA   Rowley D.L., Herman E.M.;
RT   "The upstream domain of soybean oleosin genes contains regulatory elements
RT   similar to those of legume storage proteins.";
RL   Biochim. Biophys. Acta 1345:1-4(1997).
CC   -!- FUNCTION: May have a structural role to stabilize the lipid body during
CC       desiccation of the seed by preventing coalescence of the oil. Probably
CC       interacts with both lipid and phospholipid moieties of lipid bodies.
CC       May also provide recognition signals for specific lipase anchorage in
CC       lipolysis during seedling growth.
CC   -!- SUBCELLULAR LOCATION: Lipid droplet. Membrane; Multi-pass membrane
CC       protein. Note=Surface of oil bodies. Oleosins exist at a monolayer
CC       lipid/water interface.
CC   -!- SIMILARITY: Belongs to the oleosin family. {ECO:0000305}.
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DR   EMBL; X60773; CAA43183.1; -; mRNA.
DR   EMBL; U09119; AAA17855.1; -; Unassigned_DNA.
DR   PIR; S17936; S17936.
DR   RefSeq; NP_001238518.1; NM_001251589.1.
DR   AlphaFoldDB; P29531; -.
DR   SMR; P29531; -.
DR   STRING; 3847.GLYMA16G07800.1; -.
DR   PRIDE; P29531; -.
DR   EnsemblPlants; KRH07172; KRH07172; GLYMA_16G071800.
DR   GeneID; 548081; -.
DR   Gramene; KRH07172; KRH07172; GLYMA_16G071800.
DR   KEGG; gmx:548081; -.
DR   eggNOG; ENOG502S1R0; Eukaryota.
DR   HOGENOM; CLU_101983_1_0_1; -.
DR   InParanoid; P29531; -.
DR   OrthoDB; 1510479at2759; -.
DR   Proteomes; UP000008827; Chromosome 16.
DR   Genevisible; P29531; GM.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0012511; C:monolayer-surrounded lipid storage body; IBA:GO_Central.
DR   GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR   GO; GO:0050826; P:response to freezing; IBA:GO_Central.
DR   GO; GO:0010344; P:seed oilbody biogenesis; IBA:GO_Central.
DR   InterPro; IPR000136; Oleosin.
DR   PANTHER; PTHR33203; PTHR33203; 1.
DR   Pfam; PF01277; Oleosin; 1.
DR   PROSITE; PS00811; OLEOSINS; 1.
PE   2: Evidence at transcript level;
KW   Lipid droplet; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..223
FT                   /note="P24 oleosin isoform B"
FT                   /id="PRO_0000108143"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          158..164
FT                   /note="1-1"
FT   REPEAT          165..171
FT                   /note="1-2"
FT   REPEAT          183..193
FT                   /note="2-1"
FT   REPEAT          194..204
FT                   /note="2-2"
FT   REGION          1..57
FT                   /note="Polar"
FT   REGION          58..129
FT                   /note="Hydrophobic"
FT   REGION          158..171
FT                   /note="2 X 7 AA tandem repeats"
FT   REGION          167..223
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          183..204
FT                   /note="2 X 11 AA tandem repeats"
FT   COMPBIAS        167..212
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   223 AA;  23393 MW;  5590D653FAB3334D CRC64;
     MTTVPPHSVQ VHTTTHRYEA GVVPPARFEA PRYEAGIKAP SSIYHSERGP TTSQVLAVVA
     GLPVGGILLL LAGLTLAGTL TGLVVATPLF IIFSPVLIPA TVAIGLAVAG FLTSGVFGLT
     ALSSFSWILN YIRETQPASE NLAAAAKHHL AEAAEYVGQK TKEVGQKTKE VGQDIQSKAQ
     DTREAAARDA RDAREAAARD ARDAKVEARD VKRTTVTATT ATA