ID   A28_VAR67               Reviewed;         146 AA.
AC   P0DSX7; P33847;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Envelope protein A28 homolog;
DE   AltName: Full=Protein A31;
GN   ORFNames=A31.5L, A31L, A32L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1666548;
RA   Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA   Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA   Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT   "Creation of a clone library of fragments from the natural variola virus
RT   and study of the structural and functional organization of viral genes from
RT   a circle of hosts.";
RL   Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein required for virus entry into host cell and
CC       for cell-cell fusion (syncytium formation). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       III membrane protein {ECO:0000305}. Note=Component of the intracellular
CC       mature virion (IMV) outer membrane. {ECO:0000250}.
CC   -!- PTM: Contains two intramolecular disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific pathway
CC       that operates on the cytoplasmic side of the MV membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae A28 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X69198; CAA49076.1; -; Genomic_DNA.
DR   EMBL; X67115; CAA47502.1; -; Genomic_DNA.
DR   PIR; S46858; S46858.
DR   RefSeq; NP_042179.1; NC_001611.1.
DR   GeneID; 1486509; -.
DR   KEGG; vg:1486509; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039663; P:membrane fusion involved in viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR007664; Poxvirus_A28.
DR   Pfam; PF04584; Pox_A28; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Fusion of virus membrane with host membrane; Late protein;
KW   Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral penetration into host cytoplasm; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..146
FT                   /note="Envelope protein A28 homolog"
FT                   /id="PRO_0000099301"
FT   TRANSMEM        1..21
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        22..146
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   146 AA;  16244 MW;  B8121A3A5D75A365 CRC64;
     MNSLSIFFIV VATAAVCLLF IQGYSIYENY GNIKEFNATH AAFEYSKSIG GTPALDRRVQ
     DVNDTISDVK QKWRCVAYPG NGFVSASIFG FQAEVGPNNT RSIRKFNTMA QCIDFTFSDV
     INIDIYNPCV APNINNVECQ FLKSVL