OLF15_MOUSE
ID OLF15_MOUSE Reviewed; 312 AA.
AC P23275; Q496W6; Q8VFF0;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 4.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Olfactory receptor 15;
DE AltName: Full=Odorant receptor OR3;
DE AltName: Full=Olfactory receptor 256-17;
GN Name=Olfr15; Synonyms=Mor256-17;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=1384038; DOI=10.1073/pnas.89.19.8948;
RA Nef P., Hermans-Borgmeyer I., Artieres-Pin H., Beasley L., Dionne V.E.,
RA Heinemann S.F.;
RT "Spatial pattern of receptor expression in the olfactory epithelium.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:8948-8952(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11875048; DOI=10.1093/hmg/11.5.535;
RA Young J.M., Friedman C., Williams E.M., Ross J.A., Tonnes-Priddy L.,
RA Trask B.J.;
RT "Different evolutionary processes shaped the mouse and human olfactory
RT receptor gene families.";
RL Hum. Mol. Genet. 11:535-546(2002).
RN [3]
RP ERRATUM OF PUBMED:11875048.
RA Young J.M., Friedman C., Williams E.M., Ross J.A., Tonnes-Priddy L.,
RA Trask B.J.;
RL Hum. Mol. Genet. 11:1683-1683(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11802173; DOI=10.1038/nn800;
RA Zhang X., Firestein S.;
RT "The olfactory receptor gene superfamily of the mouse.";
RL Nat. Neurosci. 5:124-133(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14611657; DOI=10.1186/gb-2003-4-11-r71;
RA Young J.M., Shykind B.M., Lane R.P., Tonnes-Priddy L., Ross J.A.,
RA Walker M., Williams E.M., Trask B.J.;
RT "Odorant receptor expressed sequence tags demonstrate olfactory expression
RT of over 400 genes, extensive alternate splicing and unequal expression
RT levels.";
RL Genome Biol. 4:R71.1-R71.13(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-312.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15342743; DOI=10.1523/jneurosci.2588-04.2004;
RA Strotmann J., Levai O., Fleischer J., Schwarzenbacher K., Breer H.;
RT "Olfactory receptor proteins in axonal processes of chemosensory neurons.";
RL J. Neurosci. 24:7754-7761(2004).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=25901328; DOI=10.1073/pnas.1503054112;
RA Block E., Jang S., Matsunami H., Sekharan S., Dethier B., Ertem M.Z.,
RA Gundala S., Pan Y., Li S., Li Z., Lodge S.N., Ozbil M., Jiang H.,
RA Penalba S.F., Batista V.S., Zhuang H.;
RT "Implausibility of the vibrational theory of olfaction.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:E2766-E2774(2015).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=29659735; DOI=10.1093/chemse/bjy022;
RA Zhang R., Pan Y., Ahmed L., Block E., Zhang Y., Batista V.S., Zhuang H.;
RT "A Multispecific Investigation of the Metal Effect in Mammalian Odorant
RT Receptors for Sulfur-containing Compounds.";
RL Chem. Senses 43:357-366(2018).
CC -!- FUNCTION: Olfactory receptor that is activated by the binding of
CC organosulfur odorants with thioether groups such as
CC (methylthio)methanetiol (MTMT) (PubMed:29659735). Also binds odorants
CC acetophenone and benzaldehyde (PubMed:25901328). The activity of this
CC receptor is mediated by G proteins which activate adenylyl cyclase
CC (Probable). May be involved in the molecular processes underlying
CC fasciculation and targeting of olfactory axons (PubMed:15342743).
CC {ECO:0000269|PubMed:15342743, ECO:0000269|PubMed:25901328,
CC ECO:0000269|PubMed:29659735, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15342743,
CC ECO:0000305|PubMed:25901328, ECO:0000305|PubMed:29659735}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Olfactory epithelium. Present in various
CC subcellular compartments of the olfactory sensory neurons, particularly
CC in the axonal processes and neve terminals.
CC {ECO:0000269|PubMed:1384038, ECO:0000269|PubMed:15342743}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M84005; AAA39862.1; -; Genomic_DNA.
DR EMBL; AY073576; AAL61239.1; -; Genomic_DNA.
DR EMBL; AY317256; AAP70766.1; -; Genomic_DNA.
DR EMBL; BC100680; AAI00681.1; -; mRNA.
DR EMBL; BC100685; AAI00686.1; -; mRNA.
DR EMBL; BC115838; AAI15839.1; -; mRNA.
DR CCDS; CCDS27910.1; -.
DR PIR; A46247; A46247.
DR RefSeq; NP_032788.2; NM_008762.2.
DR AlphaFoldDB; P23275; -.
DR SMR; P23275; -.
DR STRING; 10090.ENSMUSP00000079720; -.
DR GlyGen; P23275; 1 site.
DR PhosphoSitePlus; P23275; -.
DR PaxDb; P23275; -.
DR PRIDE; P23275; -.
DR Antibodypedia; 24106; 55 antibodies from 16 providers.
DR DNASU; 18312; -.
DR Ensembl; ENSMUST00000080917; ENSMUSP00000079720; ENSMUSG00000059043.
DR Ensembl; ENSMUST00000214238; ENSMUSP00000149504; ENSMUSG00000059043.
DR Ensembl; ENSMUST00000214590; ENSMUSP00000150757; ENSMUSG00000059043.
DR GeneID; 18312; -.
DR KEGG; mmu:18312; -.
DR UCSC; uc007xys.1; mouse.
DR CTD; 18312; -.
DR MGI; MGI:106182; Olfr15.
DR VEuPathDB; HostDB:ENSMUSG00000059043; -.
DR eggNOG; ENOG502RCDM; Eukaryota.
DR GeneTree; ENSGT01050000244803; -.
DR HOGENOM; CLU_012526_1_2_1; -.
DR InParanoid; P23275; -.
DR OMA; QLEMVFF; -.
DR OrthoDB; 1136762at2759; -.
DR PhylomeDB; P23275; -.
DR TreeFam; TF336512; -.
DR Reactome; R-MMU-381753; Olfactory Signaling Pathway.
DR BioGRID-ORCS; 18312; 2 hits in 65 CRISPR screens.
DR ChiTaRS; Olfr15; mouse.
DR PRO; PR:P23275; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; P23275; protein.
DR Bgee; ENSMUSG00000059043; Expressed in epithelium of nasal septum and 9 other tissues.
DR Genevisible; P23275; MM.
DR GO; GO:0005938; C:cell cortex; IDA:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0005549; F:odorant binding; IDA:UniProtKB.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:UniProtKB.
DR GO; GO:0050907; P:detection of chemical stimulus involved in sensory perception; IDA:UniProtKB.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISA:MGI.
DR GO; GO:0007608; P:sensory perception of smell; ISA:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000725; Olfact_rcpt.
DR Pfam; PF13853; 7tm_4; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00245; OLFACTORYR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Olfaction; Receptor; Reference proteome; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..312
FT /note="Olfactory receptor 15"
FT /id="PRO_0000150813"
FT TOPO_DOM 1..24
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..100
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..120
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..200
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..222
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..261
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..292
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..312
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 97..189
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 11
FT /note="S -> T (in Ref. 1; AAA39862)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 34320 MW; DC7E30EBBD0750E9 CRC64;
MEVDSNSSSG SFILMGVSDH PHLEIIFFAV ILASYLLTLV GNLTIILLSR LDARLHTPMY
FFLSNLSSLD LAFTTSSVPQ MLKNLWGPDK TISYGGCVTQ LYVFLWLGAT ECILLVVMAF
DRYVAVCRPL HYMTVMNPRL CWGLAAISWL GGLGNSVIQS TFTLQLPFCG HRKVDNFLCE
VPAMIKLACG DTSLNEAVLN GVCTFFTVVP VSVILVSYCF IAQAVMKIRS VEGRRKAFNT
CVSHLVVVFL FYGSAIYGYL LPAKSSNQSQ GKFISLFYSV VTPMVNPLIY TLRNKEVKGA
LGRLLGKGRG AS
