OLF16_MOUSE
ID OLF16_MOUSE Reviewed; 309 AA.
AC Q62007;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Olfactory receptor 16;
GN Name=Olfr16 {ECO:0000312|MGI:MGI:106648};
GN Synonyms=Mor23 {ECO:0000303|PubMed:8619840};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:CAA63545.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=8619840; DOI=10.1006/bbrc.1996.0580;
RA Asai H., Kasai H., Matsuda Y., Yamazaki N., Nagawa F., Sakano H.,
RA Tsuboi A.;
RT "Genomic structure and transcription of a murine odorant receptor gene:
RT differential initiation of transcription in the olfactory and testicular
RT cells.";
RL Biochem. Biophys. Res. Commun. 221:240-247(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14611657; DOI=10.1186/gb-2003-4-11-r71;
RA Young J.M., Shykind B.M., Lane R.P., Tonnes-Priddy L., Ross J.A.,
RA Walker M., Williams E.M., Trask B.J.;
RT "Odorant receptor expressed sequence tags demonstrate olfactory expression
RT of over 400 genes, extensive alternate splicing and unequal expression
RT levels.";
RL Genome Biol. 4:R71.1-R71.13(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION.
RX PubMed=10097159; DOI=10.1073/pnas.96.7.4040;
RA Touhara K., Sengoku S., Inaki K., Tsuboi A., Hirono J., Sato T., Sakano H.,
RA Haga T.;
RT "Functional identification and reconstitution of an odorant receptor in
RT single olfactory neurons.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:4040-4045(1999).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15522887; DOI=10.1242/jcs.01507;
RA Fukuda N., Yomogida K., Okabe M., Touhara K.;
RT "Functional characterization of a mouse testicular olfactory receptor and
RT its role in chemosensing and in regulation of sperm motility.";
RL J. Cell Sci. 117:5835-5845(2004).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=19922870; DOI=10.1016/j.devcel.2009.09.004;
RA Griffin C.A., Kafadar K.A., Pavlath G.K.;
RT "MOR23 promotes muscle regeneration and regulates cell adhesion and
RT migration.";
RL Dev. Cell 17:649-661(2009).
RN [8]
RP FUNCTION.
RX PubMed=25791473; DOI=10.1016/j.bbrc.2015.03.046;
RA Kim S.H., Yoon Y.C., Lee A.S., Kang N., Koo J., Rhyu M.R., Park J.H.;
RT "Expression of human olfactory receptor 10J5 in heart aorta, coronary
RT artery, and endothelial cells and its functional role in angiogenesis.";
RL Biochem. Biophys. Res. Commun. 460:404-408(2015).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=28842679; DOI=10.1038/s41598-017-10379-x;
RA Tong T., Ryu S.E., Min Y., de March C.A., Bushdid C., Golebiowski J.,
RA Moon C., Park T.;
RT "Olfactory receptor 10J5 responding to alpha-cedrene regulates hepatic
RT steatosis via the cAMP-PKA pathway.";
RL Sci. Rep. 7:9471-9471(2017).
CC -!- FUNCTION: Olfactory receptor. Activated by the synthetic floral
CC odorant, lyral, and by alpha-cedrene, a sesquiterpene constituent of
CC cedarwood oil (PubMed:25791473, PubMed:28842679, PubMed:10097159). Its
CC activation increases intracellular Ca(2+) (PubMed:25791473,
CC PubMed:28842679, PubMed:10097159, PubMed:15522887). Acts as a key
CC regulator of myogenesis through its actions on cell migration and
CC adhesion by activating the Ca(2+)-dependent AKT signal transduction
CC pathway (PubMed:19922870). Acts also as a regulator of angiogenesis
CC (PubMed:25791473). Moreover, plays a role in the regulation of lipid
CC accumulation in hepatocytes via the cAMP-PKA pathway (PubMed:28842679).
CC Involved in sperm chemotaxis and motility (PubMed:15522887).
CC {ECO:0000269|PubMed:10097159, ECO:0000269|PubMed:15522887,
CC ECO:0000269|PubMed:19922870, ECO:0000269|PubMed:25791473,
CC ECO:0000269|PubMed:28842679}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28842679};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the olfactory epithelium as well as in
CC the testis (PubMed:8619840). Expressed in round spermatids during
CC stages VI-VIII of spermatogenesis (PubMed:15522887).
CC {ECO:0000269|PubMed:15522887, ECO:0000269|PubMed:8619840}.
CC -!- DEVELOPMENTAL STAGE: Up-regulated during myogenesis in vitro and muscle
CC regeneration in vivo. {ECO:0000269|PubMed:19922870}.
CC -!- MISCELLANEOUS: The human olfactory receptor 10J5 (OR10J5) is the
CC ortholog of mouse olfr16. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; X92969; CAA63545.1; -; Genomic_DNA.
DR EMBL; AY318634; AAP71794.1; -; Genomic_DNA.
DR EMBL; BC119298; AAI19299.1; -; mRNA.
DR EMBL; BC119300; AAI19301.1; -; mRNA.
DR CCDS; CCDS15521.1; -.
DR RefSeq; NP_032789.1; NM_008763.2.
DR AlphaFoldDB; Q62007; -.
DR SMR; Q62007; -.
DR STRING; 10090.ENSMUSP00000041524; -.
DR PaxDb; Q62007; -.
DR Antibodypedia; 20471; 135 antibodies from 24 providers.
DR Ensembl; ENSMUST00000038432; ENSMUSP00000041524; ENSMUSG00000037924.
DR Ensembl; ENSMUST00000215254; ENSMUSP00000149249; ENSMUSG00000037924.
DR GeneID; 18313; -.
DR KEGG; mmu:18313; -.
DR UCSC; uc007dqz.2; mouse.
DR CTD; 18313; -.
DR MGI; MGI:106648; Olfr16.
DR VEuPathDB; HostDB:ENSMUSG00000037924; -.
DR eggNOG; ENOG502RU27; Eukaryota.
DR GeneTree; ENSGT00940000159272; -.
DR HOGENOM; CLU_012526_1_2_1; -.
DR InParanoid; Q62007; -.
DR OMA; ILATNNC; -.
DR OrthoDB; 1043779at2759; -.
DR PhylomeDB; Q62007; -.
DR TreeFam; TF337624; -.
DR Reactome; R-MMU-381753; Olfactory Signaling Pathway.
DR BioGRID-ORCS; 18313; 1 hit in 65 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q62007; protein.
DR Bgee; ENSMUSG00000037924; Expressed in spermatid and 21 other tissues.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
DR GO; GO:0016021; C:integral component of membrane; ISA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:UniProtKB.
DR GO; GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0006935; P:chemotaxis; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISA:MGI.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IDA:UniProtKB.
DR GO; GO:0055088; P:lipid homeostasis; IMP:UniProtKB.
DR GO; GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
DR GO; GO:0045765; P:regulation of angiogenesis; IMP:UniProtKB.
DR GO; GO:0051147; P:regulation of muscle cell differentiation; IMP:UniProtKB.
DR GO; GO:0007608; P:sensory perception of smell; IDA:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000725; Olfact_rcpt.
DR Pfam; PF13853; 7tm_4; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00245; OLFACTORYR.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Angiogenesis; Cell membrane; Chemotaxis; Disulfide bond;
KW G-protein coupled receptor; Membrane; Myogenesis; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..309
FT /note="Olfactory receptor 16"
FT /id="PRO_0000454829"
FT TOPO_DOM 1..27
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 28..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 58..78
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..84
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 85..105
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 132..152
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..203
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 204..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 236..256
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..270
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 271..291
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..309
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DISULFID 97..178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 309 AA; 34256 MW; BEE5CF666D0F7D68 CRC64;
MQRNNFTEVI EFVFLGFSSF GKHQITLFVV FLTIYILTLA GNIIIVTITH IDHHLHTPMY
FFLSMLASSE TVYTLVIVPR MLSSLIFYNL PISLAGCATQ MFFFVTLATN NCFLLTAMGY
DRYVAICNPL RYTIIMSKGM CALLVCGSLG TGLVMAVLHV PAMFHLPFCG TVVEHFFCDI
YPVMKLSCVD TTVNEIINYG VSSFVILVPI GLIFISYVLI VSSILKIVST EGQKKAFATC
ASHLTVVIVH YGCASIAYLK PKSESSVEKD LLLSVTYTII TPLLNPVVYS LRNKEVKDAL
CRAVGRNTS
