OLHYD_ELIME
ID OLHYD_ELIME Reviewed; 646 AA.
AC C7DLJ6;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Oleate hydratase;
DE EC=4.2.1.53;
DE AltName: Full=Fatty acid double bond hydratase;
DE AltName: Full=Fatty acid hydratase;
GN Name=ohyA;
OS Elizabethkingia meningoseptica (Chryseobacterium meningosepticum).
OC Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Elizabethkingia.
OX NCBI_TaxID=238;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ACTIVITY
RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, AND SUBUNIT.
RC STRAIN=3266;
RX PubMed=19465645; DOI=10.1128/jb.00306-09;
RA Bevers L.E., Pinkse M.W., Verhaert P.D., Hagen W.R.;
RT "Oleate hydratase catalyzes the hydration of a nonactivated carbon-carbon
RT bond.";
RL J. Bacteriol. 191:5010-5012(2009).
CC -!- FUNCTION: Catalyzes the hydration of oleate at its cis-9-double bond to
CC yield 10-hydroxyoctadecanoate. The hydration of unsaturated fatty acids
CC is suggested to be a detoxification mechanism and a survival strategy
CC for living in fatty acid-rich environments.
CC {ECO:0000269|PubMed:19465645}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-10-hydroxyoctadecanoate = (9Z)-octadecenoate + H2O;
CC Xref=Rhea:RHEA:21852, ChEBI:CHEBI:15377, ChEBI:CHEBI:15683,
CC ChEBI:CHEBI:30823; EC=4.2.1.53;
CC Evidence={ECO:0000269|PubMed:19465645};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC Note=Binds 1 FAD per subunit. FAD does not seem to be involved in
CC catalysis but rather in the structural stabilization of the enzyme.
CC {ECO:0000250};
CC -!- ACTIVITY REGULATION: Is not inhibited by EDTA in vitro.
CC {ECO:0000269|PubMed:19465645}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.21 mM for oleate (at 30 degrees Celsius)
CC {ECO:0000269|PubMed:19465645};
CC Vmax=0.170 umol/min/mg enzyme (at 30 degrees Celsius)
CC {ECO:0000269|PubMed:19465645};
CC Note=Due to the low solubility of oleate in Tris buffer, the values
CC given above are approximate, the real Vmax is most likely
CC significantly higher, whereas the real KM is expected to be much
CC lower.;
CC pH dependence:
CC Optimum pH is around 6. {ECO:0000269|PubMed:19465645};
CC -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:19465645}.
CC -!- INDUCTION: Strongly up-regulated in cells grown in the presence of
CC oleate. {ECO:0000269|PubMed:19465645}.
CC -!- SIMILARITY: Belongs to the oleate hydratase family. {ECO:0000305}.
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DR EMBL; GQ144652; ACT54545.1; -; Genomic_DNA.
DR PDB; 4UIR; X-ray; 2.75 A; A/B=1-646.
DR PDBsum; 4UIR; -.
DR AlphaFoldDB; C7DLJ6; -.
DR SMR; C7DLJ6; -.
DR STRING; 1216967.L100_11323; -.
DR eggNOG; COG4716; Bacteria.
DR BRENDA; 4.2.1.53; 1374.
DR SABIO-RK; C7DLJ6; -.
DR UniPathway; UPA00199; -.
DR GO; GO:0071949; F:FAD binding; ISS:UniProtKB.
DR GO; GO:0005504; F:fatty acid binding; IDA:UniProtKB.
DR GO; GO:0050151; F:oleate hydratase activity; IDA:UniProtKB.
DR GO; GO:0006631; P:fatty acid metabolic process; IDA:UniProtKB.
DR GO; GO:0070542; P:response to fatty acid; IDA:UniProtKB.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR010354; Oleate_hydratase.
DR PANTHER; PTHR37417; PTHR37417; 1.
DR Pfam; PF06100; MCRA; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Detoxification; FAD; Fatty acid metabolism; Flavoprotein;
KW Lipid metabolism; Lyase.
FT CHAIN 1..646
FT /note="Oleate hydratase"
FT /id="PRO_0000416454"
FT REGION 15..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 69..74
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 96
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT TURN 3..5
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 6..8
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 9..12
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 27..29
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 65..68
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 72..85
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 89..91
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 92..95
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 97..100
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 101..104
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 114..116
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 128..134
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 140..142
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 148..156
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 166..169
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 170..172
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 184..195
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 198..200
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 207..209
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 212..216
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 218..226
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 235..244
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 246..251
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 252..254
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 265..268
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 270..280
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 283..285
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 288..297
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 302..311
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 316..319
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 325..329
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 333..336
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 338..341
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 353..356
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 361..368
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 372..374
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 377..379
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 384..387
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 389..396
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 400..408
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 415..417
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 424..426
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 433..436
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 451..458
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 460..462
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 465..467
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 471..473
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 476..487
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 490..492
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 493..498
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 500..507
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 508..511
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 512..514
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 524..526
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 530..537
FT /evidence="ECO:0007829|PDB:4UIR"
FT STRAND 547..549
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 550..564
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 577..579
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 581..591
FT /evidence="ECO:0007829|PDB:4UIR"
FT TURN 592..594
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 600..607
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 613..615
FT /evidence="ECO:0007829|PDB:4UIR"
FT HELIX 630..645
FT /evidence="ECO:0007829|PDB:4UIR"
SQ SEQUENCE 646 AA; 73488 MW; 2E48182DD936251D CRC64;
MNPITSKFDK VLNASSEYGH VNHEPDSSKE QQRNTPQKSM PFSDQIGNYQ RNKGIPVQSY
DNSKIYIIGS GIAGMSAAYY FIRDGHVPAK NITFLEQLHI DGGSLDGAGN PTDGYIIRGG
REMDMTYENL WDMFQDIPAL EMPAPYSVLD EYRLINDNDS NYSKARLINN KGEIKDFSKF
GLNKMDQLAI IRLLLKNKEE LDDLTIEDYF SESFLKSNFW TFWRTMFAFE NWHSLLELKL
YMHRFLHAID GLNDLSSLVF PKYNQYDTFV TPLRKFLQEK GVNIHLNTLV KDLDIHINTE
GKVVEGIITE QDGKEVKIPV GKNDYVIVTT GSMTEDTFYG NNKTAPIIGI DNSTSGQSAG
WKLWKNLAAK SEIFGKPEKF CSNIEKSAWE SATLTCKPSA LIDKLKEYSV NDPYSGKTVT
GGIITITDSN WLMSFTCNRQ PHFPEQPDDV LVLWVYALFM DKEGNYIKKT MLECTGDEIL
AELCYHLGIE DQLENVQKNT IVRTAFMPYI TSMFMPRAKG DRPRVVPEGC KNLGLVGQFV
ETNNDVVFTM ESSVRTARIA VYKLLNLNKQ VPDINPLQYD IRHLLKAAKT LNDDKPFVGE
GLLRKVLKGT YFEHVLPAGA AEEEEHESFI AEHVNKFREW VKGIRG
