OLIG2_MOUSE
ID OLIG2_MOUSE Reviewed; 323 AA.
AC Q9EQW6; Q9JKN4;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Oligodendrocyte transcription factor 2;
DE Short=Oligo2;
GN Name=Olig2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=11091082; DOI=10.1016/s0925-4773(00)00466-4;
RA Takebayashi H., Yoshida S., Sugimori M., Kosako H., Kominami R.,
RA Nakafuku M., Nabeshima Y.;
RT "Dynamic expression of basic helix-loop-helix Olig family members:
RT implication of Olig2 in neuron and oligodendrocyte differentiation and
RT identification of a new member, Olig3.";
RL Mech. Dev. 99:143-148(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=10719889; DOI=10.1016/s0896-6273(00)80898-3;
RA Zhou Q., Wang S., Anderson D.J.;
RT "Identification of a novel family of oligodendrocyte lineage-specific basic
RT helix-loop-helix transcription factors.";
RL Neuron 25:331-343(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INDUCTION BY SHH.
RX PubMed=10719888; DOI=10.1016/s0896-6273(00)80897-1;
RA Lu Q.R., Yuk D.-I., Alberta J.A., Zhu Z., Pawlitzky I., Chan J.A.,
RA McMahon A.P., Stiles C.D., Rowitch D.H.;
RT "Sonic hedgehog-regulated oligodendrocyte lineage genes encoding bHLH
RT proteins in the mammalian central nervous system.";
RL Neuron 25:317-329(2000).
RN [5]
RP FUNCTION.
RX PubMed=11955448; DOI=10.1016/s0092-8674(02)00678-5;
RA Lu Q.R., Sun T., Zhu Z., Ma N., Garcia M., Stiles C.D., Rowitch D.H.;
RT "Common developmental requirement for Olig function indicates a motor
RT neuron/oligodendrocyte connection.";
RL Cell 109:75-86(2002).
RN [6]
RP FUNCTION.
RX PubMed=12121626; DOI=10.1016/s0960-9822(02)00926-0;
RA Takebayashi H., Nabeshima Y., Yoshida S., Chisaka O., Ikenaka K.,
RA Nabeshima Y.;
RT "The basic helix-loop-helix factor olig2 is essential for the development
RT of motoneuron and oligodendrocyte lineages.";
RL Curr. Biol. 12:1157-1163(2002).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=11960707; DOI=10.1016/s0925-4773(02)00021-7;
RA Takebayashi H., Ohtsuki T., Uchida T., Kawamoto S., Okubo K., Ikenaka K.,
RA Takeichi M., Chisaka O., Nabeshima Y.;
RT "Non-overlapping expression of Olig3 and Olig2 in the embryonic neural
RT tube.";
RL Mech. Dev. 113:169-174(2002).
RN [8]
RP INDUCTION BY NKX6-1.
RX PubMed=14602683; DOI=10.1242/dev.00868;
RA Liu R., Cai J., Hu X., Tan M., Qi Y., German M., Rubenstein J.L.R.,
RA Sander M., Qiu M.;
RT "Region-specific and stage-dependent regulation of Olig gene expression and
RT oligodendrogenesis by Nkx6.1 homeodomain transcription factor.";
RL Development 130:6221-6231(2003).
RN [9]
RP INTERACTION WITH NKX2-2, AND SUBCELLULAR LOCATION.
RX PubMed=14573534; DOI=10.1523/jneurosci.23-29-09547.2003;
RA Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D.;
RT "Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors
RT in developing neural tube associated with formation of a specific physical
RT complex.";
RL J. Neurosci. 23:9547-9556(2003).
RN [10]
RP FUNCTION, AND INTERACTION WITH ZNF488.
RX PubMed=16908628; DOI=10.1242/dev.02522;
RA Wang S.Z., Dulin J., Wu H., Hurlock E., Lee S.E., Jansson K., Lu Q.R.;
RT "An oligodendrocyte-specific zinc-finger transcription regulator cooperates
RT with Olig2 to promote oligodendrocyte differentiation.";
RL Development 133:3389-3398(2006).
CC -!- FUNCTION: Required for oligodendrocyte and motor neuron specification
CC in the spinal cord, as well as for the development of somatic motor
CC neurons in the hindbrain (PubMed:11955448, PubMed:12121626,
CC PubMed:16908628). Functions together with ZNF488 to promote
CC oligodendrocyte differentiation (PubMed:16908628). Cooperates with
CC OLIG1 to establish the pMN domain of the embryonic neural tube
CC (PubMed:11955448, PubMed:12121626). Antagonist of V2 interneuron and of
CC NKX2-2-induced V3 interneuron development (PubMed:11955448,
CC PubMed:12121626). {ECO:0000269|PubMed:11955448,
CC ECO:0000269|PubMed:12121626, ECO:0000269|PubMed:16908628}.
CC -!- SUBUNIT: Interacts with NKX2-2 (PubMed:14573534). Interacts with ZNF488
CC (PubMed:16908628). {ECO:0000269|PubMed:14573534,
CC ECO:0000269|PubMed:16908628}.
CC -!- INTERACTION:
CC Q9EQW6; P41136: Id2; NbExp=4; IntAct=EBI-1213740, EBI-309167;
CC Q9EQW6; P41139: Id4; NbExp=5; IntAct=EBI-1213740, EBI-1213725;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=The NLS contained in the
CC bHLH domain could be masked in the native form and translocation to the
CC nucleus could be mediated by interaction either with class E bHLH
CC partner protein or with NKX2-2.
CC -!- TISSUE SPECIFICITY: Expressed specifically in the brain.
CC {ECO:0000269|PubMed:11091082}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the ventral spinal cord as early as
CC 9.5 dpc. Expression becomes progressively restricted to a narrow zone
CC within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc
CC spinal cord, expressed in the oligodendrocyte progenitors of the
CC ventral ventricular zone, but not dorsal root ganglia Schwann cells.
CC Also expressed scattered in the mantle zone, likely corresponding to
CC oligodendrocyte progenitors migrating out from their site of origin. In
CC the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in
CC the ventricular and subventricular zones of the lateral and medial
CC ganglionic eminences, suggesting that expression might not be limited
CC to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the
CC gray matter, with little or no residual expression in the ventricular
CC zone. In the postnatal brain, present preferentially in the white
CC matter, such as corpus callosum and cerebellar medulla. Expressed in
CC the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5
CC dpc onward. {ECO:0000269|PubMed:10719889, ECO:0000269|PubMed:11091082}.
CC -!- INDUCTION: By SHH. Also induced by NKX6-1 in the developing spinal
CC cord, but not in the rostral hindbrain. {ECO:0000269|PubMed:10719888,
CC ECO:0000269|PubMed:14602683}.
CC -!- DOMAIN: The bHLH is essential for interaction with NKX2-2.
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DR EMBL; AB038697; BAB18907.1; -; mRNA.
DR EMBL; AF232929; AAF61722.1; -; Genomic_DNA.
DR EMBL; BC051967; AAH51967.1; -; mRNA.
DR CCDS; CCDS28323.1; -.
DR RefSeq; NP_058663.2; NM_016967.2.
DR AlphaFoldDB; Q9EQW6; -.
DR SMR; Q9EQW6; -.
DR BioGRID; 206152; 8.
DR IntAct; Q9EQW6; 4.
DR STRING; 10090.ENSMUSP00000036797; -.
DR iPTMnet; Q9EQW6; -.
DR PhosphoSitePlus; Q9EQW6; -.
DR PaxDb; Q9EQW6; -.
DR PRIDE; Q9EQW6; -.
DR ProteomicsDB; 293515; -.
DR Antibodypedia; 917; 495 antibodies from 41 providers.
DR DNASU; 50913; -.
DR Ensembl; ENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830.
DR GeneID; 50913; -.
DR KEGG; mmu:50913; -.
DR UCSC; uc007zxe.1; mouse.
DR CTD; 10215; -.
DR MGI; MGI:1355331; Olig2.
DR VEuPathDB; HostDB:ENSMUSG00000039830; -.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000161651; -.
DR HOGENOM; CLU_065376_1_0_1; -.
DR InParanoid; Q9EQW6; -.
DR OMA; PVHHPIL; -.
DR OrthoDB; 1290623at2759; -.
DR PhylomeDB; Q9EQW6; -.
DR TreeFam; TF322733; -.
DR BioGRID-ORCS; 50913; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Olig2; mouse.
DR PRO; PR:Q9EQW6; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9EQW6; protein.
DR Bgee; ENSMUSG00000039830; Expressed in future spinal cord and 102 other tissues.
DR ExpressionAtlas; Q9EQW6; baseline and differential.
DR Genevisible; Q9EQW6; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005667; C:transcription regulator complex; ISS:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0071837; F:HMG box domain binding; IPI:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0042552; P:myelination; IMP:MGI.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IDA:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0007399; P:nervous system development; IDA:MGI.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0048663; P:neuron fate commitment; IMP:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IMP:MGI.
DR GO; GO:0048714; P:positive regulation of oligodendrocyte differentiation; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0021522; P:spinal cord motor neuron differentiation; IMP:MGI.
DR GO; GO:0021529; P:spinal cord oligodendrocyte cell differentiation; IGI:MGI.
DR GO; GO:0021530; P:spinal cord oligodendrocyte cell fate specification; IDA:MGI.
DR GO; GO:0021794; P:thalamus development; IMP:MGI.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032658; Olig2.
DR PANTHER; PTHR19290:SF32; PTHR19290:SF32; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..323
FT /note="Oligodendrocyte transcription factor 2"
FT /id="PRO_0000127415"
FT DOMAIN 108..162
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..95
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 198
FT /note="L -> I (in Ref. 2; AAF61722)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="P -> PTRHGAP (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 323 AA; 32407 MW; 598AE76CB512D716 CRC64;
MDSDASLVSS RPSSPEPDDL FLPARSKGGS SSGFTGGTVS SSTPSDCPPE LSSELRGAMG
ASGAHPGDKL GGGGFKSSSS STSSSTSSAA TSSTKKDKKQ MTEPELQQLR LKINSRERKR
MHDLNIAMDG LREVMPYAHG PSVRKLSKIA TLLLARNYIL MLTNSLEEMK RLVSEIYGGH
HAGFHPSACG GLAHSAPLPT ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS
ASLPGSGLSS VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG GSGGFQHWGG MPCPCSMCQV
PPPHHHVSAM GAGTLPRLTS DAK
