OLM2A_HUMAN
ID OLM2A_HUMAN Reviewed; 652 AA.
AC Q68BL7; Q5JTM5; Q5JTM6; Q6UXW1; Q7Z5V3;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Olfactomedin-like protein 2A;
DE AltName: Full=Photomedin-1;
DE Flags: Precursor;
GN Name=OLFML2A; ORFNames=UNQ9394/PRO34319;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ALA-309.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Retina;
RA Furutani Y., Manabe R., Tsutsui K., Yamada T., Sugimoto N., Kawai J.,
RA Hayashizaki Y., Sekiguchi K.;
RT "Photomedin-1 and -2, novel extracellular glycoproteins with olfactomedin
RT domain, isolated from RIKEN full-length mouse cDNA clones by computational
RT screening.";
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND VARIANT ALA-309.
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=22913984; DOI=10.1038/ki.2012.321;
RA Sistani L., Rodriguez P.Q., Hultenby K., Uhlen M., Betsholtz C.,
RA Jalanko H., Tryggvason K., Wernerson A., Patrakka J.;
RT "Neuronal proteins are novel components of podocyte major processes and
RT their expression in glomerular crescents supports their role in crescent
RT formation.";
RL Kidney Int. 83:63-71(2013).
CC -!- SUBUNIT: Homodimer. Binds to heparin and chondroitin sulfate E.
CC {ECO:0000250|UniProtKB:Q8BHP7}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q8BHP7}.
CC Note=Localizes to the podocyte major processes. Colocalized with the
CC major process protein VIM throughout podocyte development.
CC {ECO:0000269|PubMed:22913984}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q68BL7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q68BL7-2; Sequence=VSP_029566;
CC Name=3;
CC IsoId=Q68BL7-3; Sequence=VSP_029565, VSP_029567;
CC -!- TISSUE SPECIFICITY: In the kidney expressed only by podocytes, wherein
CC they localize to major processes. {ECO:0000269|PubMed:22913984}.
CC -!- DEVELOPMENTAL STAGE: Detected at the vesicle stage of developing
CC glomeruli, expressed in invading endothelial cells in the glomerular
CC cleft at the S-shaped stage and is later expressed only at the basal
CC aspect of maturing podocytes. {ECO:0000269|PubMed:22913984}.
CC -!- PTM: May be cleaved at Lys-295 after secretion.
CC {ECO:0000250|UniProtKB:Q8BHP7}.
CC -!- PTM: O-glycosylated but not N-glycosylated.
CC {ECO:0000250|UniProtKB:Q8BHP7}.
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DR EMBL; AY358185; AAQ88552.1; -; mRNA.
DR EMBL; AB119055; BAD38864.1; -; mRNA.
DR EMBL; AL354928; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471090; EAW87596.1; -; Genomic_DNA.
DR EMBL; BC054001; AAH54001.1; -; mRNA.
DR CCDS; CCDS65129.1; -. [Q68BL7-3]
DR CCDS; CCDS6857.2; -. [Q68BL7-1]
DR RefSeq; NP_001269644.1; NM_001282715.1. [Q68BL7-3]
DR RefSeq; NP_872293.2; NM_182487.3. [Q68BL7-1]
DR AlphaFoldDB; Q68BL7; -.
DR SMR; Q68BL7; -.
DR BioGRID; 127983; 15.
DR IntAct; Q68BL7; 1.
DR STRING; 9606.ENSP00000362682; -.
DR GlyGen; Q68BL7; 1 site.
DR iPTMnet; Q68BL7; -.
DR PhosphoSitePlus; Q68BL7; -.
DR BioMuta; OLFML2A; -.
DR DMDM; 74748246; -.
DR EPD; Q68BL7; -.
DR jPOST; Q68BL7; -.
DR MassIVE; Q68BL7; -.
DR PaxDb; Q68BL7; -.
DR PeptideAtlas; Q68BL7; -.
DR PRIDE; Q68BL7; -.
DR ProteomicsDB; 65996; -. [Q68BL7-1]
DR ProteomicsDB; 65997; -. [Q68BL7-2]
DR ProteomicsDB; 65998; -. [Q68BL7-3]
DR Antibodypedia; 16348; 161 antibodies from 24 providers.
DR DNASU; 169611; -.
DR Ensembl; ENST00000288815.5; ENSP00000288815.5; ENSG00000185585.20. [Q68BL7-3]
DR Ensembl; ENST00000373580.8; ENSP00000362682.3; ENSG00000185585.20. [Q68BL7-1]
DR GeneID; 169611; -.
DR KEGG; hsa:169611; -.
DR MANE-Select; ENST00000373580.8; ENSP00000362682.3; NM_182487.4; NP_872293.2.
DR UCSC; uc004bov.5; human. [Q68BL7-1]
DR CTD; 169611; -.
DR DisGeNET; 169611; -.
DR GeneCards; OLFML2A; -.
DR HGNC; HGNC:27270; OLFML2A.
DR HPA; ENSG00000185585; Low tissue specificity.
DR MIM; 615899; gene.
DR neXtProt; NX_Q68BL7; -.
DR OpenTargets; ENSG00000185585; -.
DR PharmGKB; PA134967883; -.
DR VEuPathDB; HostDB:ENSG00000185585; -.
DR eggNOG; KOG3545; Eukaryota.
DR GeneTree; ENSGT00940000157194; -.
DR HOGENOM; CLU_024107_0_0_1; -.
DR InParanoid; Q68BL7; -.
DR OMA; PQLPFRN; -.
DR OrthoDB; 311650at2759; -.
DR PhylomeDB; Q68BL7; -.
DR TreeFam; TF351220; -.
DR PathwayCommons; Q68BL7; -.
DR SignaLink; Q68BL7; -.
DR BioGRID-ORCS; 169611; 19 hits in 1070 CRISPR screens.
DR ChiTaRS; OLFML2A; human.
DR GenomeRNAi; 169611; -.
DR Pharos; Q68BL7; Tdark.
DR PRO; PR:Q68BL7; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q68BL7; protein.
DR Bgee; ENSG00000185585; Expressed in dorsal root ganglion and 165 other tissues.
DR ExpressionAtlas; Q68BL7; baseline and differential.
DR Genevisible; Q68BL7; HS.
DR GO; GO:0031012; C:extracellular matrix; IEA:Ensembl.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0050840; F:extracellular matrix binding; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0030198; P:extracellular matrix organization; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR003112; Olfac-like_dom.
DR InterPro; IPR031231; OLFML2A.
DR PANTHER; PTHR23192:SF29; PTHR23192:SF29; 1.
DR Pfam; PF02191; OLF; 1.
DR SMART; SM00284; OLF; 1.
DR PROSITE; PS51132; OLF; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Disulfide bond; Glycoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000250|UniProtKB:Q8BHP7"
FT CHAIN 28..652
FT /note="Olfactomedin-like protein 2A"
FT /id="PRO_0000311428"
FT DOMAIN 394..652
FT /note="Olfactomedin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00446"
FT REGION 209..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 312..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 157..183
FT /evidence="ECO:0000255"
FT COMPBIAS 349..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 294..295
FT /note="Cleavage"
FT /evidence="ECO:0000250|UniProtKB:Q8BHP7"
FT DISULFID 395..582
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00446"
FT VAR_SEQ 1..214
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029565"
FT VAR_SEQ 1..39
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_029566"
FT VAR_SEQ 215..223
FT /note="ATGTGSKAQ -> MSKRDKAGK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029567"
FT VARIANT 309
FT /note="T -> A (in dbSNP:rs7874348)"
FT /evidence="ECO:0000269|PubMed:12975309,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_037250"
FT VARIANT 425
FT /note="R -> Q (in dbSNP:rs16927649)"
FT /id="VAR_037251"
SQ SEQUENCE 652 AA; 73054 MW; E3D3B61C8CC0699C CRC64;
MAAAALPPRP LLLLPLVLLL SGRPTRADSK VFGDLDQVRM TSEGSDCRCK CIMRPLSKDA
CSRVRSGRAR VEDFYTVETV SSGTDCRCSC TAPPSSLNPC ENEWKMEKLK KQAPELLKLQ
SMVDLLEGTL YSMDLMKVHA YVHKVASQMN TLEESIKANL SRENEVVKDS VRHLSEQLRH
YENHSAIMLG IKKELSRLGL QLLQKDAAAA PATPATGTGS KAQDTARGKG KDISKYGSVQ
KSFADRGLPK PPKEKLLQVE KLRKESGKGS FLQPTAKPRA LAQQQAVIRG FTYYKAGKQE
VTEAVADNTL QGTSWLEQLP PKVEGRSNSA EPNSAEQDEA EPRSSERVDL ASGTPTSIPA
TTTTATTTPT PTTSLLPTEP PSGPEVSSQG REASCEGTLR AVDPPVRHHS YGRHEGAWMK
DPAARDDRIY VTNYYYGNSL VEFRNLENFK QGRWSNMYKL PYNWIGTGHV VYQGAFYYNR
AFTKNIIKYD LRQRFVASWA LLPDVVYEDT TPWKWRGHSD IDFAVDESGL WVIYPAVDDR
DEAQPEVIVL SRLDPGDLSV HRETTWKTRL RRNSYGNCFL VCGILYAVDT YNQQEGQVAY
AFDTHTGTDA RPQLPFLNEH AYTTQIDYNP KERVLYAWDN GHQLTYTLHF VV
