OLNB2_BRANA
ID OLNB2_BRANA Reviewed; 183 AA.
AC P29526; P81097;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Oleosin-B2;
DE AltName: Full=Oleosin-C98;
DE Contains:
DE RecName: Full=Pollen coat protein B2;
DE Flags: Fragment;
GN Name=OlnB2; Synonyms=C98;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Anther;
RX PubMed=8374615; DOI=10.1111/j.1365-313x.1993.00629.x;
RA Roberts M.R., Hodge R., Ross J.H.E., Sorensen A., Murphy D.J., Draper J.,
RA Scott R.;
RT "Characterization of a new class of oleosins suggests a male gametophyte-
RT specific lipid storage pathway.";
RL Plant J. 3:629-636(1993).
RN [2]
RP PROTEIN SEQUENCE OF 104-120, AND FUNCTION.
RC STRAIN=cv. Topas; TISSUE=Pollen;
RX PubMed=9680961;
RA Murphy D.J., Ross J.H.E.;
RT "Biosynthesis, targeting and processing of oleosin-like proteins, which are
RT major pollen coat components in Brassica napus.";
RL Plant J. 13:1-16(1998).
CC -!- FUNCTION: Many of the major pollen coat proteins are derived from
CC endoproteolytic cleavage of oleosin-like proteins.
CC {ECO:0000269|PubMed:9680961}.
CC -!- SUBCELLULAR LOCATION: Lipid droplet. Membrane; Multi-pass membrane
CC protein. Note=Surface of oil bodies. Oleosins exist at a monolayer
CC lipid/water interface.
CC -!- TISSUE SPECIFICITY: The full-length protein is found in the tapetal
CC lipid bodies of immature anthers, the proteolytically cleaved C-
CC terminal product is found on the coats of pollen grains. Not present in
CC seeds. {ECO:0000269|PubMed:8374615}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing buds, until the pollen is
CC near to maturity. {ECO:0000269|PubMed:8374615}.
CC -!- SIMILARITY: Belongs to the oleosin family. {ECO:0000305}.
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DR EMBL; X67142; CAA47623.1; -; mRNA.
DR PIR; S24960; S24960.
DR AlphaFoldDB; P29526; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0012511; C:monolayer-surrounded lipid storage body; IEA:InterPro.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR InterPro; IPR000136; Oleosin.
DR PANTHER; PTHR33203; PTHR33203; 1.
DR Pfam; PF01277; Oleosin; 1.
DR PROSITE; PS00811; OLEOSINS; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Lipid droplet; Membrane; Transmembrane;
KW Transmembrane helix.
FT CHAIN <1..183
FT /note="Oleosin-B2"
FT /id="PRO_0000108135"
FT CHAIN 104..183
FT /note="Pollen coat protein B2"
FT /id="PRO_0000284379"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION <1..23
FT /note="Polar"
FT REGION 24..95
FT /note="Hydrophobic"
FT CONFLICT 108
FT /note="L -> I (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT NON_TER 1
SQ SEQUENCE 183 AA; 18149 MW; 198A5D3B6DF3045A CRC64;
QASIFSRFFR MFSFIFPFVN VIKLIIASVT SLVCLAFSCV ALGGSAVALI VSTPLFIMFS
PILVPATIAT TLLASGLMAG TTLGLTGIGL IMGLVRTAGG VSLLQSPLRK IIVNRIKARL
GGGGGGSRLA RLKKILGLLN KLRGMGAGGA AAPAAEPAPA AEAAPAAEAA PAAAPAAAPA
AAP
