OLNB3_BRANA
ID OLNB3_BRANA Reviewed; 424 AA.
AC Q42626;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Oleosin-B3;
DE Contains:
DE RecName: Full=Pollen coat protein B3;
GN Name=OlnB3 {ECO:0000303|PubMed:8653113};
GN Synonyms=STA 41-2 {ECO:0000312|EMBL:AAA70400.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA70400.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Westar {ECO:0000312|EMBL:AAA70400.1};
RC TISSUE=Tapetum {ECO:0000312|EMBL:AAA70400.1};
RX PubMed=7849761; DOI=10.1046/j.1365-313x.1994.6060927.x;
RA Robert L.S., Gerster J., Allard S., Cass L., Simmonds J.;
RT "Molecular characterization of two Brassica napus genes related to oleosins
RT which are highly expressed in the tapetum.";
RL Plant J. 6:927-933(1994).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 1-10 AND 109-129, FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Topas {ECO:0000269|PubMed:9680961};
RC TISSUE=Pollen {ECO:0000269|PubMed:9680961};
RX PubMed=9680961;
RA Murphy D.J., Ross J.H.E.;
RT "Biosynthesis, targeting and processing of oleosin-like proteins, which are
RT major pollen coat components in Brassica napus.";
RL Plant J. 13:1-16(1998).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 109-128, FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Topas {ECO:0000269|PubMed:8653113};
RC TISSUE=Pollen {ECO:0000269|PubMed:8653113};
RX PubMed=8653113; DOI=10.1046/j.1365-313x.1996.9050625.x;
RA Ross J.H.E., Murphy D.J.;
RT "Characterization of anther-expressed genes encoding a major class of
RT extracellular oleosin-like proteins in the pollen coat of Brassicaceae.";
RL Plant J. 9:625-637(1996).
CC -!- FUNCTION: Many of the major pollen coat proteins are derived from
CC endoproteolytic cleavage of oleosin-like proteins.
CC {ECO:0000269|PubMed:8653113, ECO:0000269|PubMed:9680961}.
CC -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250}. Membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=Surface
CC of oil bodies. Oleosins exist at a monolayer lipid/water interface (By
CC similarity). {ECO:0000250|UniProtKB:P29526}.
CC -!- TISSUE SPECIFICITY: The full-length protein is found in the tapetal
CC lipid bodies of immature anthers, the proteolytically cleaved C-
CC terminal product is found on the coats of pollen grains. No expression
CC is detected in other flower organs, siliques or seedlings.
CC {ECO:0000269|PubMed:7849761, ECO:0000269|PubMed:9680961}.
CC -!- DEVELOPMENTAL STAGE: Only expressed in early developing buds and
CC anthers, mRNA is first detected in 2-3 mm buds, expression levels peak
CC in 4-5 mm buds and are absent in later stages of development.
CC {ECO:0000269|PubMed:7849761, ECO:0000269|PubMed:8653113,
CC ECO:0000269|PubMed:9680961}.
CC -!- SIMILARITY: Belongs to the oleosin family. {ECO:0000255}.
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DR EMBL; L33282; AAA70400.1; -; mRNA.
DR PIR; T08093; T08093.
DR RefSeq; NP_001303026.1; NM_001316097.1.
DR AlphaFoldDB; Q42626; -.
DR SMR; Q42626; -.
DR EnsemblPlants; CDY68880; CDY68880; GSBRNA2T00080952001.
DR GeneID; 106449581; -.
DR Gramene; CDY68880; CDY68880; GSBRNA2T00080952001.
DR KEGG; bna:106449581; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0012511; C:monolayer-surrounded lipid storage body; IEA:InterPro.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR InterPro; IPR000136; Oleosin.
DR PANTHER; PTHR33203; PTHR33203; 3.
DR Pfam; PF01277; Oleosin; 1.
DR PROSITE; PS00811; OLEOSINS; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Lipid droplet; Membrane; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..424
FT /note="Oleosin-B3"
FT /id="PRO_0000284754"
FT CHAIN 109..424
FT /note="Pollen coat protein B3"
FT /evidence="ECO:0000269|PubMed:8653113"
FT /id="PRO_0000284755"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 111..120
FT /note="1-1"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 121..130
FT /note="1-2"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 131..140
FT /note="1-3"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 141..150
FT /note="1-4"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 196..202
FT /note="2-1"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 203..209
FT /note="2-2"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 210..216
FT /note="2-3"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 217..223
FT /note="2-4"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 241..258
FT /note="3-1"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 259..276
FT /note="3-2"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 277..294
FT /note="3-3"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 301..318
FT /note="3-4"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 319..336
FT /note="3-5"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 337..354
FT /note="3-6"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 396..400
FT /note="4-1"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 401..405
FT /note="4-2"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 406..410
FT /note="4-3"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REPEAT 411..415
FT /note="4-4"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REGION 1..37
FT /note="Polar"
FT /evidence="ECO:0000255"
FT REGION 38..119
FT /note="Hydrophobic"
FT /evidence="ECO:0000255"
FT REGION 111..150
FT /note="4 X 10 AA tandem repeats of I-P-[EV]-S-I-K-P-S-N-
FT [IV]"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REGION 164..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 196..223
FT /note="4 X 7 AA tandem repeats of E-[SD]-[KT]-H-G-K-G"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REGION 241..354
FT /note="6 X 18 AA tandem repeats of [KR]-H-[EG]-[SG]-G-G-
FT [SA]-[PSA]-M-G-G-G-K-H-[GE]-S-[GV]-G"
FT /evidence="ECO:0000269|PubMed:7849761"
FT REGION 396..415
FT /note="4 X 5 AA tandem repeats of S-S-D-G-S"
FT /evidence="ECO:0000269|PubMed:7849761"
FT COMPBIAS 164..241
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 387..418
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 424 AA; 42086 MW; 751C6D971FBBA6B0 CRC64;
MRNEIQNETA QTDQTQGSMF SFFNLFPFLL PMFEVIKMVV ASVASVVYLG FAGVTLSGSA
VALAVSTPLF IIFSPILLPA IAATTVLAAG LGSKKVAAAP AASPSLSLLG IPESIKPSNV
IPESIKPSNI IPESIKPSNI IPVSIKPSNI KDKIKDTIGK VKNKIKAKQE EKSKGKSEDS
SKGKGKSKGE DTTTDEDKHG KGESKHGKGE SKHGKGESTH GKGGKHGSEG SSMDEGKHGG
KHGSGGSPMG GGKHGSGGKH ESGGSPMGGG KHGSGGKHES GGASMGGGKH ESVGKHGSGG
KHESGGSPMG GGKHGSGGKH ESGGASMGGG KHGSGGRHEG GGSAMGGGKH GSGGKHGSEG
KHGGEGSSMG KNSLSKNKKE FHYRGQAMDA SSTSESSDGS SSDGSSSDGS SSDGSSHGSG
GKHI
