OLNB6_BRANA
ID OLNB6_BRANA Reviewed; 375 AA.
AC Q43402; P93066; Q43397;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 3.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Oleosin-B6;
DE AltName: Full=Oleosin-B11;
DE AltName: Full=Oleosin-B13;
DE Contains:
DE RecName: Full=Pollen coat protein B6;
DE AltName: Full=Pollen coat protein B11;
DE AltName: Full=Pollen coat protein B13;
GN Name=OlnB6 {ECO:0000303|PubMed:8653113};
GN Synonyms=OlnB11 {ECO:0000303|PubMed:8653113},
GN OlnB13 {ECO:0000303|PubMed:9225865};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 141-160,
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Topas {ECO:0000269|PubMed:8653113};
RC TISSUE=Flower bud {ECO:0000269|PubMed:8653113}, and
RC Pollen {ECO:0000269|PubMed:8653113};
RX PubMed=8653113; DOI=10.1046/j.1365-313x.1996.9050625.x;
RA Ross J.H.E., Murphy D.J.;
RT "Characterization of anther-expressed genes encoding a major class of
RT extracellular oleosin-like proteins in the pollen coat of Brassicaceae.";
RL Plant J. 9:625-637(1996).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
RX PubMed=9225865; DOI=10.1023/a:1005840824926;
RA Hong H.P., Ross J.H.E., Gerster J.L., Rigas S., Datla R.S.S.,
RA Hatzopoulos P., Scoles G., Keller W., Murphy D.J., Robert L.S.;
RT "Promoter sequences from two different Brassica napus tapetal oleosin-like
RT genes direct tapetal expression of beta-glucuronidase in transgenic
RT Brassica plants.";
RL Plant Mol. Biol. 34:549-555(1997).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 141-155, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Topas {ECO:0000269|PubMed:9680961};
RC TISSUE=Pollen {ECO:0000269|PubMed:9680961};
RX PubMed=9680961;
RA Murphy D.J., Ross J.H.E.;
RT "Biosynthesis, targeting and processing of oleosin-like proteins, which are
RT major pollen coat components in Brassica napus.";
RL Plant J. 13:1-16(1998).
CC -!- FUNCTION: Many of the major pollen coat proteins are derived from
CC endoproteolytic cleavage of oleosin-like proteins.
CC {ECO:0000269|PubMed:8653113, ECO:0000269|PubMed:9680961}.
CC -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250}. Membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=Surface
CC of oil bodies. Oleosins exist at a monolayer lipid/water interface (By
CC similarity). {ECO:0000250|UniProtKB:P29526}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:9225865};
CC IsoId=Q43402-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:8653113};
CC IsoId=Q43402-2; Sequence=VSP_024474;
CC -!- TISSUE SPECIFICITY: The full-length protein is found in the tapetal
CC lipid bodies of immature anthers, the proteolytically cleaved C-
CC terminal product is found on the coats of pollen grains. Not found in
CC flowers, developing embryos or leaf tissue.
CC {ECO:0000269|PubMed:8653113, ECO:0000269|PubMed:9680961}.
CC -!- DEVELOPMENTAL STAGE: Only found in buds 3-5 mm long.
CC {ECO:0000269|PubMed:8653113}.
CC -!- SIMILARITY: Belongs to the oleosin family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA64805.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X95554; CAA64800.1; -; mRNA.
DR EMBL; X95559; CAA64805.1; ALT_INIT; mRNA.
DR EMBL; Y08986; CAA70173.1; -; Genomic_DNA.
DR PIR; T08109; T08109.
DR PIR; T08132; T08132.
DR PIR; T08134; T08134.
DR RefSeq; NP_001302642.1; NM_001315713.1.
DR AlphaFoldDB; Q43402; -.
DR EnsemblPlants; CDX81060; CDX81060; GSBRNA2T00134499001.
DR GeneID; 106443049; -.
DR Gramene; CDX81060; CDX81060; GSBRNA2T00134499001.
DR KEGG; bna:106443049; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0012511; C:monolayer-surrounded lipid storage body; IEA:InterPro.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR InterPro; IPR000136; Oleosin.
DR PANTHER; PTHR33203; PTHR33203; 3.
DR Pfam; PF01277; Oleosin; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Lipid droplet; Membrane;
KW Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..375
FT /note="Oleosin-B6"
FT /id="PRO_0000273522"
FT CHAIN 141..375
FT /note="Pollen coat protein B6"
FT /evidence="ECO:0000269|PubMed:8653113"
FT /id="PRO_0000273523"
FT TRANSMEM 21..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 207..209
FT /note="1"
FT /evidence="ECO:0000255"
FT REPEAT 210..212
FT /note="2"
FT /evidence="ECO:0000255"
FT REPEAT 213..215
FT /note="3"
FT /evidence="ECO:0000255"
FT REPEAT 216..218
FT /note="4"
FT /evidence="ECO:0000255"
FT REPEAT 219..221
FT /note="5"
FT /evidence="ECO:0000255"
FT REPEAT 222..224
FT /note="6"
FT /evidence="ECO:0000255"
FT REPEAT 225..227
FT /note="7"
FT /evidence="ECO:0000255"
FT REPEAT 228..230
FT /note="8"
FT /evidence="ECO:0000255"
FT REPEAT 231..233
FT /note="9"
FT /evidence="ECO:0000255"
FT REPEAT 234..236
FT /note="10"
FT /evidence="ECO:0000255"
FT REPEAT 237..239
FT /note="11"
FT /evidence="ECO:0000255"
FT REPEAT 240..242
FT /note="12"
FT /evidence="ECO:0000255"
FT REPEAT 243..245
FT /note="13"
FT /evidence="ECO:0000255"
FT REPEAT 246..248
FT /note="14"
FT /evidence="ECO:0000255"
FT REPEAT 249..251
FT /note="15"
FT /evidence="ECO:0000255"
FT REPEAT 252..254
FT /note="16"
FT /evidence="ECO:0000255"
FT REPEAT 255..257
FT /note="17"
FT /evidence="ECO:0000255"
FT REPEAT 258..260
FT /note="18"
FT /evidence="ECO:0000255"
FT REPEAT 261..263
FT /note="19"
FT /evidence="ECO:0000255"
FT REPEAT 264..266
FT /note="20"
FT /evidence="ECO:0000255"
FT REPEAT 267..269
FT /note="21"
FT /evidence="ECO:0000255"
FT REPEAT 270..272
FT /note="22"
FT /evidence="ECO:0000255"
FT REPEAT 273..275
FT /note="23"
FT /evidence="ECO:0000255"
FT REGION 1..32
FT /note="Polar"
FT /evidence="ECO:0000255"
FT REGION 33..112
FT /note="Hydrophobic"
FT /evidence="ECO:0000255"
FT REGION 144..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 207..275
FT /note="23 X 3 AA approximate tandem repeats of P-A-A"
FT /evidence="ECO:0000255"
FT REGION 303..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..283
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..328
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..344
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..375
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 185..345
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8653113"
FT /id="VSP_024474"
FT CONFLICT 2
FT /note="K -> E (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
FT CONFLICT 13..14
FT /note="QL -> LS (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="F -> Y (in Ref. 1; CAA64805)"
FT /evidence="ECO:0000305"
FT CONFLICT 28
FT /note="I -> L (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="T -> S (in Ref. 1; CAA64805)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="K -> E (in Ref. 1; CAA64805)"
FT /evidence="ECO:0000305"
FT CONFLICT 163..165
FT /note="Missing (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="E -> D (in Ref. 1; CAA64805)"
FT /evidence="ECO:0000305"
FT CONFLICT 353..355
FT /note="EKS -> DKK (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
FT CONFLICT 360..364
FT /note="Missing (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
FT CONFLICT 373..374
FT /note="ES -> DE (in Ref. 1; CAA64805/CAA64800)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 37993 MW; CB12D512FDFF991D CRC64;
MKEEIQNETA QTQLQREGRM FSFLFPVIEV IKVVMASVAS VVFLGFGGVT LACSAVALAV
STPLFIIFSP ILVPATIATT LLATGLGAGT TLGVTGMGLL MRLIKHPGKE GAASAPAAQP
SFLSLLEMPN FIKSKMLERL IHIPGVGKKS EGRGESKGKK GKKGKSEHGR GKHEGEGKSK
GRKGHRMGVN PENNPPPAGA PPTGSPPAAP AAPEAPAAPA APAAPAAPAA PAAPAAPEDP
AAPAAPEAPA TPAAPPAPAA APAPAAPAAP PAPAAPPRPP SFLSLLEMPS FIKSKLIEAL
INIPGFGKKS NDRGKSKGGK KSKGKGKSNG RGKHEGEGKS KSRKSKSRGK DKEKSKGKGI
FGRSSRKGSS DDESS
