ID   OLSB_AGRFC              Reviewed;         293 AA.
AC   Q7D1N1;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=L-ornithine N(alpha)-acyltransferase {ECO:0000305};
DE            EC=2.3.2.30 {ECO:0000250|UniProtKB:Q92SJ1};
GN   Name=olsB {ECO:0000303|PubMed:22958119};
GN   OrderedLocusNames=Atu0344 {ECO:0000312|EMBL:AAK86161.2};
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
RN   [3]
RP   DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=22958119; DOI=10.1111/j.1462-2920.2012.02867.x;
RA   Vences-Guzman M.A., Guan Z., Bermudez-Barrientos J.R., Geiger O.,
RA   Sohlenkamp C.;
RT   "Agrobacteria lacking ornithine lipids induce more rapid tumour
RT   formation.";
RL   Environ. Microbiol. 15:895-906(2013).
CC   -!- FUNCTION: Catalyzes the first step in the biosynthesis of ornithine
CC       lipids, which are phosphorus-free membrane lipids. Catalyzes the 3-
CC       hydroxyacyl-acyl carrier protein-dependent acylation of ornithine to
CC       form lyso-ornithine lipid (LOL). {ECO:0000250|UniProtKB:Q92SJ1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-hydroxyacyl-[ACP] + L-ornithine = a lyso-ornithine
CC         lipid + H(+) + holo-[ACP]; Xref=Rhea:RHEA:20633, Rhea:RHEA-COMP:9685,
CC         Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:46911,
CC         ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:138482;
CC         EC=2.3.2.30; Evidence={ECO:0000250|UniProtKB:Q92SJ1};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20634;
CC         Evidence={ECO:0000250|UniProtKB:Q92SJ1};
CC   -!- PATHWAY: Lipid metabolism. {ECO:0000269|PubMed:22958119}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant is unable to produce ornithine
CC       lipids. Mutant promotes earlier tumor formation on the plant host.
CC       {ECO:0000269|PubMed:22958119}.
CC   -!- SIMILARITY: Belongs to the acetyltransferase family. OlsB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE007869; AAK86161.2; -; Genomic_DNA.
DR   RefSeq; NP_353376.2; NC_003062.2.
DR   RefSeq; WP_006310189.1; NC_003062.2.
DR   AlphaFoldDB; Q7D1N1; -.
DR   STRING; 176299.Atu0344; -.
DR   EnsemblBacteria; AAK86161; AAK86161; Atu0344.
DR   GeneID; 66220554; -.
DR   KEGG; atu:Atu0344; -.
DR   PATRIC; fig|176299.10.peg.335; -.
DR   eggNOG; COG3176; Bacteria.
DR   HOGENOM; CLU_058962_1_0_5; -.
DR   OMA; YLRLGAW; -.
DR   PhylomeDB; Q7D1N1; -.
DR   BioCyc; AGRO:ATU0344-MON; -.
DR   Proteomes; UP000000813; Chromosome circular.
DR   GO; GO:0043810; F:ornithine-acyl [acyl carrier protein] N-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   SUPFAM; SSF55729; SSF55729; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW   Transferase.
FT   CHAIN           1..293
FT                   /note="L-ornithine N(alpha)-acyltransferase"
FT                   /id="PRO_0000452110"
SQ   SEQUENCE   293 AA;  32805 MW;  2C3D0A14BF08E3C4 CRC64;
     MVAEIFNHDI CENNVVISPR SETAQDNEGL FGRIGTLETR LARNEREIDA AQSVRYRVFV
     EEMKARLPAE AMRRKRDFDA WDSVCDHLLV LDKSIEGDSE DQIVGTYRLL RQETALANNG
     FYSASEFDIA GLVARHPGKR FMELGRSCVL PEYRTKRTVE LLWQGNWAYA VKHRMDAMIG
     CASFPGVQPE AHALALSFLH HNCLAKGEWE AVALPELYHE MDLVPVEALN TRKALNAMPP
     LIKGYMRLGA MFGSGAVVDH AFNTTDVLVV LPVSSIAGRY ISYYGGEAER ING