ID   OLSB_BRUA2              Reviewed;         281 AA.
AC   Q2YNY9;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=L-ornithine N(alpha)-acyltransferase {ECO:0000305};
DE            EC=2.3.2.30 {ECO:0000269|PubMed:21249206};
GN   Name=olsB {ECO:0000303|PubMed:21249206};
GN   OrderedLocusNames=BAB1_0147 {ECO:0000312|EMBL:CAJ10103.1};
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=2308;
RX   PubMed=21249206; DOI=10.1371/journal.pone.0016030;
RA   Palacios-Chaves L., Conde-Alvarez R., Gil-Ramirez Y., Zuniga-Ripa A.,
RA   Barquero-Calvo E., Chacon-Diaz C., Chaves-Olarte E., Arce-Gorvel V.,
RA   Gorvel J.P., Moreno E., de Miguel M.J., Grillo M.J., Moriyon I.,
RA   Iriarte M.;
RT   "Brucella abortus ornithine lipids are dispensable outer membrane
RT   components devoid of a marked pathogen-associated molecular pattern.";
RL   PLoS ONE 6:e16030-e16030(2011).
CC   -!- FUNCTION: Catalyzes the first step in the biosynthesis of ornithine
CC       lipids, which are phosphorus-free membrane lipids. Catalyzes the 3-
CC       hydroxyacyl-acyl carrier protein-dependent acylation of ornithine to
CC       form lyso-ornithine lipid (LOL). {ECO:0000269|PubMed:21249206}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-hydroxyacyl-[ACP] + L-ornithine = a lyso-ornithine
CC         lipid + H(+) + holo-[ACP]; Xref=Rhea:RHEA:20633, Rhea:RHEA-COMP:9685,
CC         Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:46911,
CC         ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:138482;
CC         EC=2.3.2.30; Evidence={ECO:0000269|PubMed:21249206};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20634;
CC         Evidence={ECO:0000269|PubMed:21249206};
CC   -!- PATHWAY: Lipid metabolism. {ECO:0000269|PubMed:21249206}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene abrogates lyso-ornithine
CC       lipid and ornithine lipid synthesis. It does not affect resistance to
CC       bactericidal peptides or permeability of the outer membrane. Does not
CC       influence pro-inflammatory responses or the ability to multiply
CC       intracellularly and generate chronic infections.
CC       {ECO:0000269|PubMed:21249206}.
CC   -!- SIMILARITY: Belongs to the acetyltransferase family. OlsB subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AM040264; CAJ10103.1; -; Genomic_DNA.
DR   RefSeq; WP_011265290.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YNY9; -.
DR   STRING; 359391.BAB1_0147; -.
DR   EnsemblBacteria; CAJ10103; CAJ10103; BAB1_0147.
DR   GeneID; 3786947; -.
DR   KEGG; bmf:BAB1_0147; -.
DR   PATRIC; fig|359391.4.peg.155; -.
DR   HOGENOM; CLU_058962_1_1_5; -.
DR   OMA; YLRLGAW; -.
DR   Proteomes; UP000002719; Chromosome I.
DR   GO; GO:0043810; F:ornithine-acyl [acyl carrier protein] N-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   SUPFAM; SSF55729; SSF55729; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW   Transferase.
FT   CHAIN           1..281
FT                   /note="L-ornithine N(alpha)-acyltransferase"
FT                   /id="PRO_0000452111"
SQ   SEQUENCE   281 AA;  31730 MW;  B20BA0EB3CF613B3 CRC64;
     MSGLEAQQAL FASNGDAIIL GRIGSLEVRL ANSRAAIQAA QELRFRVFFE EMGARKETIE
     AVEQRDADRF DTICDHLLVY DTALPVPEHQ QIVGTYRLMR NEQAEKALGF YSADEYDVQR
     LKLSRPNLRL LELGRSCVKP EYRSKRTVEL LWQGAWAYCR RHSIDVMFGC ASFHGAVPAA
     HALGLSFLHH NCRATDDWDV RALPHRYQAM DLMPKEAINN KVALFSMPPL VKGYLRLGAM
     IGDGAVIDEA FGTTDVFIIL PIERISSRYI SYYGAEANRF V