OLSB_RHIME
ID OLSB_RHIME Reviewed; 296 AA.
AC Q92SJ1;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=L-ornithine N(alpha)-acyltransferase {ECO:0000305};
DE EC=2.3.2.30 {ECO:0000269|PubMed:15341653};
DE AltName: Full=Ornithine lipid synthesis protein B {ECO:0000305};
GN Name=olsB {ECO:0000303|PubMed:15341653};
GN ORFNames=SMc01127 {ECO:0000312|EMBL:CAC41830.1};
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND DISRUPTION PHENOTYPE.
RX PubMed=15341653; DOI=10.1111/j.1365-2958.2004.04240.x;
RA Gao J.L., Weissenmayer B., Taylor A.M., Thomas-Oates J., Lopez-Lara I.M.,
RA Geiger O.;
RT "Identification of a gene required for the formation of lyso-ornithine
RT lipid, an intermediate in the biosynthesis of ornithine-containing
RT lipids.";
RL Mol. Microbiol. 53:1757-1770(2004).
CC -!- FUNCTION: Catalyzes the first step in the biosynthesis of ornithine
CC lipids, which are phosphorus-free membrane lipids. Catalyzes the 3-
CC hydroxyacyl-acyl carrier protein-dependent acylation of ornithine to
CC form lyso-ornithine lipid (LOL). {ECO:0000269|PubMed:15341653}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3R)-hydroxyacyl-[ACP] + L-ornithine = a lyso-ornithine
CC lipid + H(+) + holo-[ACP]; Xref=Rhea:RHEA:20633, Rhea:RHEA-COMP:9685,
CC Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:46911,
CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:138482;
CC EC=2.3.2.30; Evidence={ECO:0000269|PubMed:15341653};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20634;
CC Evidence={ECO:0000269|PubMed:15341653};
CC -!- PATHWAY: Lipid metabolism. {ECO:0000269|PubMed:15341653}.
CC -!- DISRUPTION PHENOTYPE: Disruption mutant is unable to synthesize
CC ornithine lipids. It can still induce nitrogen-fixing nodules on
CC plants, but it shows delayed nodulation if compared to the wild type.
CC {ECO:0000269|PubMed:15341653}.
CC -!- SIMILARITY: Belongs to the acetyltransferase family. OlsB subfamily.
CC {ECO:0000305}.
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DR EMBL; AL591688; CAC41830.1; -; Genomic_DNA.
DR RefSeq; NP_384499.1; NC_003047.1.
DR RefSeq; WP_010968546.1; NC_003047.1.
DR AlphaFoldDB; Q92SJ1; -.
DR SMR; Q92SJ1; -.
DR STRING; 266834.SMc01127; -.
DR EnsemblBacteria; CAC41830; CAC41830; SMc01127.
DR GeneID; 61601872; -.
DR KEGG; sme:SMc01127; -.
DR PATRIC; fig|266834.11.peg.1766; -.
DR eggNOG; COG3176; Bacteria.
DR HOGENOM; CLU_058962_1_0_5; -.
DR OMA; YLRLGAW; -.
DR BioCyc; MetaCyc:MON-16663; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0043810; F:ornithine-acyl [acyl carrier protein] N-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR SUPFAM; SSF55729; SSF55729; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW Transferase.
FT CHAIN 1..296
FT /note="L-ornithine N(alpha)-acyltransferase"
FT /id="PRO_0000452113"
SQ SEQUENCE 296 AA; 32670 MW; B6460E02EA352622 CRC64;
MTIELLDSMG VVDTSNAYIR KAVAAPASDV LGRIANLETR LARSAAEIDA AQAVRYRVFV
EEMKAQVAPE AGRRKRDIDS WDAICDHLLV LDTSIEGDAE EQIVGTYRLL RQDVAERTGG
FYSASEFAIG ELLSRHPGKR FMELGRSCVL PEYRTKRTVE LLWQGNWAYA LKHGIDAMFG
CGSFPGVVPE EHALALSFLH HNVRVRDEWA VSARPELYRT MDLMPPEAIN PKKALAALPP
LIKGYMRLGA MVGDGAVVDQ AFRTTDVLIV LPIGKISGRY LNYYGADAGR FSSPVS
