OM14_YEAST
ID OM14_YEAST Reviewed; 134 AA.
AC P38325; D6VQM5;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Mitochondrial outer membrane protein OM14;
DE AltName: Full=Outer membrane protein of 14 kDa;
GN Name=OM14; OrderedLocusNames=YBR230C; ORFNames=YBR1527;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP PROTEIN SEQUENCE OF 1-12, AND SUBCELLULAR LOCATION.
RX PubMed=16689936; DOI=10.1111/j.1742-4658.2006.05171.x;
RA Burri L., Vascotto K., Gentle I.E., Chan N.C., Beilharz T., Stapleton D.I.,
RA Ramage L., Lithgow T.;
RT "Integral membrane proteins in the mitochondrial outer membrane of
RT Saccharomyces cerevisiae.";
RL FEBS J. 273:1507-1515(2006).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12 AND SER-15, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:14576278,
CC ECO:0000269|PubMed:16689936}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:14576278,
CC ECO:0000269|PubMed:16689936}.
CC -!- MISCELLANEOUS: Present with 4750 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z36099; CAA85193.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07345.1; -; Genomic_DNA.
DR PIR; S46106; S46106.
DR RefSeq; NP_009789.1; NM_001178578.1.
DR AlphaFoldDB; P38325; -.
DR BioGRID; 32925; 88.
DR DIP; DIP-4946N; -.
DR IntAct; P38325; 2.
DR STRING; 4932.YBR230C; -.
DR TCDB; 9.B.235.1.1; the mitochondrial outer membrane protein-14 (om14) family.
DR iPTMnet; P38325; -.
DR MaxQB; P38325; -.
DR PaxDb; P38325; -.
DR PRIDE; P38325; -.
DR EnsemblFungi; YBR230C_mRNA; YBR230C; YBR230C.
DR GeneID; 852531; -.
DR KEGG; sce:YBR230C; -.
DR SGD; S000000434; OM14.
DR VEuPathDB; FungiDB:YBR230C; -.
DR eggNOG; ENOG502S89U; Eukaryota.
DR HOGENOM; CLU_156741_0_0_1; -.
DR InParanoid; P38325; -.
DR OMA; YANHNAR; -.
DR BioCyc; YEAST:G3O-29162-MON; -.
DR PRO; PR:P38325; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38325; protein.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:1990593; F:nascent polypeptide-associated complex binding; IDA:SGD.
DR GO; GO:0006626; P:protein targeting to mitochondrion; IMP:SGD.
DR GO; GO:0033750; P:ribosome localization; IMP:SGD.
DR InterPro; IPR039454; OM14.
DR PANTHER; PTHR38402; PTHR38402; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..134
FT /note="Mitochondrial outer membrane protein OM14"
FT /id="PRO_0000202518"
FT TRANSMEM 71..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 12
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17287358"
FT MOD_RES 15
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17287358"
SQ SEQUENCE 134 AA; 14610 MW; 2B4E4E35C283473D CRC64;
MSATAKHDSN ASPNSDSEDG HHHNNKKECA IEYLKARLNS ASAVACGYLQ AFVSKTQDFA
KVCFLELQNP VVLVNLLLHS SVVCYLCNGY ANHNARFLKG KPNSTVLATT AGALGLLTLD
GIISKKYYSR YDKK
