OM45_YEAST
ID OM45_YEAST Reviewed; 393 AA.
AC P16547; D6VVF0;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Mitochondrial outer membrane protein OM45;
DE AltName: Full=Outer membrane protein of 45 kDa;
GN Name=OM45; OrderedLocusNames=YIL136W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2687271; DOI=10.1016/s0021-9258(19)30050-x;
RA Yaffe M.P., Jensen R.E., Guido E.C.;
RT "The major 45-kDa protein of the yeast mitochondrial outer membrane is not
RT essential for cell growth or mitochondrial function.";
RL J. Biol. Chem. 264:21091-21096(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP PROTEIN SEQUENCE OF 129-136 AND 251-257, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 201238 / W303-1B;
RX PubMed=11502169; DOI=10.1021/bi010277r;
RA Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S.,
RA Schmitter J.-M.;
RT "Yeast mitochondrial dehydrogenases are associated in a supramolecular
RT complex.";
RL Biochemistry 40:9758-9769(2001).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP INTERACTION WITH AIM5; FCJ1 AND MOS1.
RX PubMed=21987634; DOI=10.1083/jcb.201107053;
RA Hoppins S., Collins S.R., Cassidy-Stone A., Hummel E., Devay R.M.,
RA Lackner L.L., Westermann B., Schuldiner M., Weissman J.S., Nunnari J.;
RT "A mitochondrial-focused genetic interaction map reveals a scaffold-like
RT complex required for inner membrane organization in mitochondria.";
RL J. Cell Biol. 195:323-340(2011).
CC -!- SUBUNIT: Interacts with AIM5, FCJ1 and MOS1.
CC {ECO:0000269|PubMed:21987634}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:11502169}.
CC -!- MISCELLANEOUS: Present with 6490 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z38059; CAA86142.1; -; Genomic_DNA.
DR EMBL; M31796; AAA34827.1; -; Genomic_DNA.
DR EMBL; AY692773; AAT92792.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08416.1; -; Genomic_DNA.
DR PIR; A34185; S48398.
DR RefSeq; NP_012130.1; NM_001179484.1.
DR AlphaFoldDB; P16547; -.
DR BioGRID; 34855; 73.
DR DIP; DIP-5289N; -.
DR IntAct; P16547; 5.
DR MINT; P16547; -.
DR STRING; 4932.YIL136W; -.
DR iPTMnet; P16547; -.
DR MaxQB; P16547; -.
DR PaxDb; P16547; -.
DR PRIDE; P16547; -.
DR EnsemblFungi; YIL136W_mRNA; YIL136W; YIL136W.
DR GeneID; 854670; -.
DR KEGG; sce:YIL136W; -.
DR SGD; S000001398; OM45.
DR VEuPathDB; FungiDB:YIL136W; -.
DR eggNOG; ENOG502QTPN; Eukaryota.
DR HOGENOM; CLU_035516_0_0_1; -.
DR InParanoid; P16547; -.
DR OMA; IEYERYG; -.
DR BioCyc; YEAST:G3O-31387-MON; -.
DR PRO; PR:P16547; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P16547; protein.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..393
FT /note="Mitochondrial outer membrane protein OM45"
FT /id="PRO_0000058041"
FT TOPO_DOM 1..4
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 86..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 186..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..105
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..214
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..251
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 51
FT /note="P -> A (in Ref. 1; AAA34827)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 393 AA; 44580 MW; 77154D522E578225 CRC64;
MSSRIIVGSA ALAAAITASI MVREQKAKGQ RREGNVSAYY NGQEYGSSAP PQLGKLHNIK
QGIKEDALSL KDALLGVSQK AREEAPKVTK RVISPEEDAQ TRKQLGQKAK DSSSQSIFNW
GFSEAERRKA IAIGEFDTAK KRFEEAVDRN EKELLSTVMR EKKAALDRAS IEYERYGRAR
DFNELSDKLD QQERNSNPLK RLLKNNTGDA NTEEAAARSV QGWGDTAQEF GREELEEAKR
NASSEPSEAQ KRLDELKKIK EKGWFGYNKG EQSEQQIAER VARGLEGWGE TAAQLSKDEM
DDLRWNYENS KKQLDKNVSD AMDSLSKAKE DLKQYGSHWW SGWTSKVDND KQALKDEAQK
KYDEALKKYD EAKNKFKEWN DKGDGKFWSS KKD
