OMB_DROME
ID OMB_DROME Reviewed; 972 AA.
AC Q24432; Q27917; Q9W4K5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2003, sequence version 3.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Optomotor-blind protein;
DE AltName: Full=Lethal(1)optomotor-blind;
DE Short=L(1)omb;
DE AltName: Full=Protein bifid;
GN Name=bi; Synonyms=OMB; ORFNames=CG3578;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=1741374; DOI=10.1073/pnas.89.4.1199;
RA Pflugfelder G.O., Roth H., Poeck B., Kerscher S., Schwarz H., Jonschker B.,
RA Heisenberg M.;
RT "The lethal(1)optomotor-blind gene of Drosophila melanogaster is a major
RT organizer of optic lobe development: isolation and characterization of the
RT gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:1199-1203(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 1-447, AND MUTAGENESIS.
RC TISSUE=Larva;
RX PubMed=8492800; DOI=10.1007/bf00291990;
RA Poeck B., Balles J., Pflugfelder G.O.;
RT "Transcript identification in the optomotor-blind locus of Drosophila
RT melanogaster by intragenic recombination mapping and PCR-aided sequence
RT analysis of lethal point mutations.";
RL Mol. Gen. Genet. 238:325-332(1993).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-887, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Essential protein that may function as a transcription
CC regulator. Vital for pupal development. Required for proper development
CC of the optic lobes and wings, and abdominal pigmentation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201}.
CC -!- TISSUE SPECIFICITY: In third-instar larvae, expressed in the brain
CC region that will develop into optic lobes and more weakly in the
CC thoracic part of the ventral ganglion.
CC -!- DEVELOPMENTAL STAGE: The peak periods of expression are: mid-
CC embryogenesis, the second day of pupal development and in the adult.
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DR EMBL; M81796; AAA28736.1; -; mRNA.
DR EMBL; AE014298; AAF45946.2; -; Genomic_DNA.
DR EMBL; S61732; AAB26697.1; -; Genomic_DNA.
DR EMBL; S61727; AAB26697.1; JOINED; Genomic_DNA.
DR EMBL; S61729; AAB26697.1; JOINED; Genomic_DNA.
DR EMBL; S61744; AAB26699.1; -; Genomic_DNA.
DR EMBL; S61743; AAB26699.1; JOINED; Genomic_DNA.
DR EMBL; S61955; AAB26699.1; JOINED; Genomic_DNA.
DR PIR; A40213; A40213.
DR RefSeq; NP_001162662.1; NM_001169191.1.
DR RefSeq; NP_001162663.1; NM_001169192.2.
DR RefSeq; NP_525070.2; NM_080331.3.
DR AlphaFoldDB; Q24432; -.
DR SMR; Q24432; -.
DR BioGRID; 57896; 17.
DR IntAct; Q24432; 2.
DR STRING; 7227.FBpp0301040; -.
DR iPTMnet; Q24432; -.
DR PaxDb; Q24432; -.
DR EnsemblMetazoa; FBtr0070672; FBpp0070640; FBgn0000179.
DR GeneID; 31379; -.
DR KEGG; dme:Dmel_CG3578; -.
DR CTD; 31379; -.
DR FlyBase; FBgn0000179; bi.
DR VEuPathDB; VectorBase:FBgn0000179; -.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00940000163374; -.
DR HOGENOM; CLU_011202_0_0_1; -.
DR InParanoid; Q24432; -.
DR SignaLink; Q24432; -.
DR BioGRID-ORCS; 31379; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 31379; -.
DR PRO; PR:Q24432; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000179; Expressed in ventral nervous system (Drosophila) and 35 other tissues.
DR ExpressionAtlas; Q24432; baseline and differential.
DR Genevisible; Q24432; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:FlyBase.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0001745; P:compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0048066; P:developmental pigmentation; TAS:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0035265; P:organ growth; IMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048100; P:wing disc anterior/posterior pattern formation; IMP:FlyBase.
DR GO; GO:0007472; P:wing disc morphogenesis; IMP:FlyBase.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR021101; Optomoror-blind_N.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF11078; Optomotor-blind; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..972
FT /note="Optomotor-blind protein"
FT /id="PRO_0000184466"
FT DNA_BIND 332..513
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 44..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 263..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 645..676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 805..889
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 918..972
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..177
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..193
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..528
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 529..544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 654..671
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 845..859
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 934..954
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 887
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 10
FT /note="L -> F (in Ref. 2; AAF45946)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="P -> A (in Ref. 2; AAF45946)"
FT /evidence="ECO:0000305"
FT CONFLICT 511
FT /note="F -> L (in Ref. 1; AAA28736)"
FT /evidence="ECO:0000305"
FT CONFLICT 522
FT /note="K -> NCYR (in Ref. 1; AAA28736)"
FT /evidence="ECO:0000305"
FT CONFLICT 820
FT /note="Missing (in Ref. 1; AAA28736)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 972 AA; 102557 MW; 61D2B3576DC6B853 CRC64;
MRYDVQELLL HQSAEDPFAR FANGMAYHPF LQLTQRPTDF SVSSLLTAGS NNNNSGNTNS
GNNNSNSNNN TNSNTNNTNN LVAVSPTGGG AQLSPQSNHS SSNTTTTSNT NNSSSNNNNN
NSTHNNNNNH TNNNNNNNNN TSQKQGHHLS TTEEPPSPAG TPPPTIVGLP PIPPPNNNSS
SSSSNNSASA AAHPSHHPTA AHHSPSTGAA APPAGPTGLP PPTPPHHLQQ QQQQQQHPAP
PPPPYFPAAA LAALAGSPAG PHPGLYPGGG LRFPPHHPGA HPHAHHLGSA YTTAEDVVLA
SAVAHQLHPA MRPLRALQPE DDGVVDDPKV TLEGKDLWEK FHKLGTEMVI TKSGRQMFPQ
MKFRVSGLDA KAKYILLLDI VAADDYRYKF HNSRWMVAGK ADPEMPKRMY IHPDSPTTGE
QWMQKVVSFH KLKLTNNISD KHGFVSTTIL NSMHKYQPRF HLVRANDILK LPYSTFRTYV
FKETEFIAVT AYQNEKITQL KIDNNPFAKG FRDTGAGKRE KKQALMSNRG SDSDKLNPTH
VSSSRAPLHL GHAGRPPHLH PHAALLDNQQ DDDDKLLDVV GPPQSPLLPL SHSLQQMHAH
QHSAALAAWF NHLAGAGAGA SEHAAAAAAN ASAEDALRRR LQADADVERD GSDSSCSESV
GGSTGGAFRP TSTGSPKEAV GAAAAAAAAG LNPGGGSYPS PNISVGPPIH PSPHLLPYLY
PHGLYPPPHL GLLHNPAAAA AMSPAGLNPG LLFNAQLALA AQHPALFGHA YAAAGHTPVS
PLQGLKSHRF SPYSLPGSLG SAFDAVTPGS NANRSGDPPG GGGGGLGGGV VENGPRSLSS
SPRPRPASHS PPTRPISMSP TTPPSLMKQP RGGGAGAGVA QSQHSPSELK SMEKMVNGLE
VQHNGSAAAA AAALQLAEEA AQHHHHTQAH HQQQQHQSHH QQQHHQQPAQ PHPHHQTHLH
SHHGATTGGT DQ
