OMCBD_CHLTR
ID OMCBD_CHLTR Reviewed; 547 AA.
AC P0CC04; P18151;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 53.
DE RecName: Full=Large cysteine-rich periplasmic protein OmcB;
DE Short=Large-CRP;
DE AltName: Full=60 kDa cysteine-rich OMP;
DE AltName: Full=60 kDa outer membrane protein;
DE AltName: Full=Cysteine-rich outer membrane protein;
DE Short=CRP;
DE Flags: Precursor;
GN Name=omcB; Synonyms=omp2, omp2B; OrderedLocusNames=CT_443;
OS Chlamydia trachomatis (strain D/UW-3/Cx).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=272561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B/Tw-5/OT;
RX PubMed=2287277; DOI=10.1111/j.1365-2958.1990.tb02065.x;
RA Allen J.E., Cerrone M.C., Beatty P.R., Stephens R.S.;
RT "Cysteine-rich outer membrane proteins of Chlamydia trachomatis display
RT compensatory sequence changes between biovariants.";
RL Mol. Microbiol. 4:1543-1550(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D/UW-3/Cx;
RX PubMed=9784136; DOI=10.1126/science.282.5389.754;
RA Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L.,
RA Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.;
RT "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia
RT trachomatis.";
RL Science 282:754-759(1998).
CC -!- FUNCTION: In elementary bodies (EBs, the infectious stage, which is
CC able to survive outside the host cell) provides the structural
CC integrity of the outer envelope through disulfide cross-links with the
CC small cysteine-rich protein and the major outer membrane protein. It
CC has been described in publications as the Sarkosyl-insoluble COMC
CC (Chlamydia outer membrane complex), and serves as the functional
CC equivalent of peptidoglycan (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of a disulfide cross-linked outer membrane complex (COMC)
CC composed of the major outer membrane porin (MOMP), the small cysteine-
CC rich protein (OmcA) and the large cysteine-rich periplasmic protein
CC (OmcB). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: It is present but the disulfide bonds are reduced
CC in the intracellular reticulate bodies (RBs).
CC -!- CAUTION: Was thought to be an outer membrane protein as it is part of a
CC disulfide cross-linked complex that is insoluble in the detergent
CC Sarkosyl; however based on experiments in C.psittaci it is likely to be
CC periplasmic. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC68042.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE001273; AAC68042.1; ALT_INIT; Genomic_DNA.
DR PIR; D71515; D71515.
DR RefSeq; NP_219955.1; NC_000117.1.
DR AlphaFoldDB; P0CC04; -.
DR STRING; 813.O172_02415; -.
DR EnsemblBacteria; AAC68042; AAC68042; CT_443.
DR GeneID; 884223; -.
DR KEGG; ctr:CT_443; -.
DR PATRIC; fig|272561.5.peg.478; -.
DR HOGENOM; CLU_029611_0_0_0; -.
DR InParanoid; P0CC04; -.
DR OMA; CEAEFVS; -.
DR Proteomes; UP000000431; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005201; F:extracellular matrix structural constituent; IEA:InterPro.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR003506; Chlam_OMP6.
DR InterPro; IPR001434; DUF11.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF03504; Chlam_OMP6; 1.
DR Pfam; PF01345; DUF11; 3.
DR PRINTS; PR01336; CHLAMIDIAOM6.
DR TIGRFAMs; TIGR01451; B_ant_repeat; 2.
PE 2: Evidence at transcript level;
KW Cell shape; Disulfide bond; Periplasm; Reference proteome; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..40
FT /evidence="ECO:0000255"
FT /id="PRO_0000020170"
FT CHAIN 41..547
FT /note="Large cysteine-rich periplasmic protein OmcB"
FT /id="PRO_0000020171"
FT REGION 45..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..77
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 233
FT /note="I -> V (in strain: B/TW-05/OT)"
SQ SEQUENCE 547 AA; 58694 MW; 42719B4BDCEDCCEA CRC64;
MNKLIRRAVT IFAVTSVASL FASGVLETSM AESLSTNVIS LADTKAKDNT SHKSKKARKN
HSKETPVDRK EVAPVHESKA TGPKQDSCFG RMYTVKVNDD RNVEITQAVP EYATVGSPYP
IEITATGKRD CVDVIITQQL PCEAEFVRSD PATTPTADGK LVWKIDRLGQ GEKSKITVWV
KPLKEGCCFT AATVCACPEI RSVTKCGQPA ICVKQEGPEN ACLRCPVVYK INIVNQGTAT
ARNVVVENPV PDGYAHSSGQ RVLTFTLGDM QPGEHRTITV EFCPLKRGRA TNIATVSYCG
GHKNTASVTT VINEPCVQVS IAGADWSYVC KPVEYVISVS NPGDLVLRDV VVEDTLSPGV
TVLEAAGAQI SCNKVVWTVK ELNPGESLQY KVLVRAQTPG QFTNNVVVKS CSDCGTCTSC
AEATTYWKGV AATHMCVVDT CDPVCVGENT VYRICVTNRG SAEDTNVSLM LKFSKELQPV
SFSGPTKGTI TGNTVVFDSL PRLGSKETVE FSVTLKAVSA GDARGEAILS SDTLTVPVSD
TENTHIY