OMCBE_CHLTH
ID OMCBE_CHLTH Reviewed; 547 AA.
AC P23603;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Large cysteine-rich periplasmic protein OmcB, serovar E;
DE Short=Large-CRP;
DE AltName: Full=60 kDa CRP;
DE AltName: Full=60 kDa outer membrane protein;
DE AltName: Full=Cysteine-rich outer membrane protein;
DE Flags: Precursor;
GN Name=omcB; Synonyms=omp2, omp2B;
OS Chlamydia trachomatis.
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=813;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=E/DK-20;
RX PubMed=2251143; DOI=10.1093/nar/18.22.6713;
RA Coles A.M., Allan I., Pearce J.H.;
RT "The nucleotide and derived amino acid sequence of the omp2 gene of
RT Chlamydia trachomatis serovar E.";
RL Nucleic Acids Res. 18:6713-6713(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BOUR / Serovar E;
RX PubMed=1997423; DOI=10.1128/iai.59.3.1196-1201.1991;
RA de la Maza L.M., Fiedler T.J., Carlson E.J., Markoff B.A., Peterson E.M.;
RT "Sequence diversity of the 60-kilodalton protein and of a putative 15-
RT kilodalton protein between the trachoma and lymphogranuloma venereum
RT biovars of Chlamydia trachomatis.";
RL Infect. Immun. 59:1196-1201(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Zhang Y.-X., Caldwell H.D.;
RT "The nucleotide sequences of 10 and 60 kDa cysteine-rich outer membrane
RT protein genes of Chlamydia trachomatis serovar F.";
RL Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In elementary bodies (EBs, the infectious stage, which is
CC able to survive outside the host cell) provides the structural
CC integrity of the outer envelope through disulfide cross-links with the
CC small cysteine-rich protein and the major outer membrane protein. It
CC has been described in publications as the Sarkosyl-insoluble COMC
CC (Chlamydia outer membrane complex), and serves as the functional
CC equivalent of peptidoglycan (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of a disulfide cross-linked outer membrane complex (COMC)
CC composed of the major outer membrane porin (MOMP), the small cysteine-
CC rich protein (OmcA) and the large cysteine-rich periplasmic protein
CC (OmcB). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: It is present but the disulfide bonds are reduced
CC in the intracellular reticulate bodies (RBs).
CC -!- MISCELLANEOUS: The sequence shown is that of strain DK 20.
CC -!- CAUTION: Was thought to be an outer membrane protein as it is part of a
CC disulfide cross-linked complex that is insoluble in the detergent
CC Sarkosyl; however based on experiments in C.psittaci it is likely to be
CC periplasmic. {ECO:0000305}.
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DR EMBL; X55903; CAA39396.1; -; Genomic_DNA.
DR EMBL; X54389; CAA38259.1; -; Genomic_DNA.
DR EMBL; M85196; AAA23154.1; -; Genomic_DNA.
DR PIR; B43584; B43584.
DR RefSeq; WP_009873136.1; NZ_CVNT01000001.1.
DR AlphaFoldDB; P23603; -.
DR eggNOG; COG1361; Bacteria.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005201; F:extracellular matrix structural constituent; IEA:InterPro.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR003506; Chlam_OMP6.
DR InterPro; IPR001434; DUF11.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF03504; Chlam_OMP6; 1.
DR Pfam; PF01345; DUF11; 3.
DR PRINTS; PR01336; CHLAMIDIAOM6.
DR TIGRFAMs; TIGR01451; B_ant_repeat; 1.
PE 2: Evidence at transcript level;
KW Cell shape; Disulfide bond; Periplasm; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..40
FT /evidence="ECO:0000255"
FT /id="PRO_0000020172"
FT CHAIN 41..547
FT /note="Large cysteine-rich periplasmic protein OmcB,
FT serovar E"
FT /id="PRO_0000020173"
FT REGION 46..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..77
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 33..34
FT /note="SL -> FT (in strain: DK20)"
FT VARIANT 121
FT /note="I -> L (in strain: DK20)"
FT VARIANT 132
FT /note="V -> A (in strain: DK20)"
FT VARIANT 458
FT /note="N -> S (in strain: DK20)"
SQ SEQUENCE 547 AA; 58708 MW; 052066084F4E20AB CRC64;
MNKLIRRAVT IFAVTSVASL FASGVLETSM AESLSTNVIS LADTKAKDNT SHKSKKARKN
HSKETLVDRK EVAPVHESKA TGPKQDSCFG RMYTVKVNDD RNVEITQAVP EYATVGSPYP
IEITATGKRD CVDVIITQQL PCEAEFVRSD PATTPTADGK LVWKIDRLGQ GEKSKITVWV
KPLKEGCCFT AATVCACPEI RSVTKCGQPA ICVKQEGPEN ACLRCPVVYK INVVNQGTAI
ARNVVVENPV PDGYAHSSGQ RVLTFTLGDM QPGEHRTITV EFCPLKRGRA TNIATVSYCG
GHKNTASVTT VINEPCVQVS IAGADWSYVC KPVEYVISVS NPGDLVLRDV VVEDTLSPGV
TVLEAAGAQI SCNKVVWTVK ELNPGESLQY KVLVRAQTPG QFTNNVVVKS CSDCGTCTSC
AEATTYWKGV AATHMCVVDT CDPVCVGENT VYRICVTNRG SAEDTNVSLM LKFSKELQPV
SFSGPTKGTI TGNTVVFDSL PRLGSKETVE FSVTLKAVSA GDARGEAILS SDTLTVPVSD
TENTHIY