OMCB_CHLPN
ID OMCB_CHLPN Reviewed; 556 AA.
AC P23700; Q9JQI6;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 25-MAY-2022, entry version 143.
DE RecName: Full=Large cysteine-rich periplasmic protein OmcB;
DE Short=Large-CRP;
DE AltName: Full=60 kDa cysteine-rich OMP;
DE Short=60 kDa CRP;
DE AltName: Full=60 kDa outer membrane protein;
DE AltName: Full=Cysteine-rich outer membrane protein;
DE Flags: Precursor;
GN Name=omcB; Synonyms=omp2; OrderedLocusNames=CPn_0557, CP_0195, CpB0579;
OS Chlamydia pneumoniae (Chlamydophila pneumoniae).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=83558;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=IOL-207;
RX PubMed=2402463; DOI=10.1093/nar/18.17.5299;
RA Watson M.W., Al-Mahdawi S., Lamden P.R., Clarke I.N.;
RT "The nucleotide sequence of the 60 kDa cysteine rich outer membrane protein
RT of Chlamydia pneumoniae strain IOL-207.";
RL Nucleic Acids Res. 18:5299-5299(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=IOL-207;
RX PubMed=7582008; DOI=10.1099/13500872-141-10-2489;
RA Watson M.W., Clarke I.N., Everson J.S., Lambden P.R.;
RT "The CrP operon of Chlamydia psittaci and Chlamydia pneumoniae.";
RL Microbiology 141:2489-2497(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CWL029;
RX PubMed=10192388; DOI=10.1038/7716;
RA Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W.,
RA Olinger L., Grimwood J., Davis R.W., Stephens R.S.;
RT "Comparative genomes of Chlamydia pneumoniae and C. trachomatis.";
RL Nat. Genet. 21:385-389(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AR39;
RX PubMed=10684935; DOI=10.1093/nar/28.6.1397;
RA Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O.,
RA Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S.,
RA Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J.,
RA Gwinn M.L., Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G.,
RA Salzberg S.L., Eisen J.A., Fraser C.M.;
RT "Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae
RT AR39.";
RL Nucleic Acids Res. 28:1397-1406(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J138;
RX PubMed=10871362; DOI=10.1093/nar/28.12.2311;
RA Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K.,
RA Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.;
RT "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from
RT Japan and CWL029 from USA.";
RL Nucleic Acids Res. 28:2311-2314(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND DISCUSSION OF SEQUENCE.
RC STRAIN=J138;
RX PubMed=10839753; DOI=10.1086/315616;
RA Shirai M., Hirakawa H., Ouchi K., Tabuchi M., Kishi F., Kimoto M.,
RA Takeuchi H., Nishida J., Shibata K., Fujinaga R., Yoneda H., Matsushima H.,
RA Tanaka C., Furukawa S., Miura K., Nakazawa A., Ishii K., Shiba T.,
RA Hattori M., Kuhara S., Nakazawa T.;
RT "Comparison of outer membrane protein genes omp and pmp in the whole genome
RT sequences of Chlamydia pneumoniae isolates from Japan and the United
RT States.";
RL J. Infect. Dis. 181 Suppl. 3:S524-S527(2000).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TW-183;
RA Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P.,
RA Schneider S., Pohl T., Essig A., Marre R., Melchers K.;
RT "The genome sequence of Chlamydia pneumoniae TW183 and comparison with
RT other Chlamydia strains based on whole genome sequence analysis.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In elementary bodies (EBs, the infectious stage, which is
CC able to survive outside the host cell) provides the structural
CC integrity of the outer envelope through disulfide cross-links with the
CC small cysteine-rich protein and the major outer membrane porin. It has
CC been described in publications as the Sarkosyl-insoluble COMC
CC (Chlamydia outer membrane complex), and serves as the functional
CC equivalent of peptidoglycan (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of a disulfide cross-linked outer membrane complex (COMC)
CC composed of the major outer membrane porin (MOMP), the small cysteine-
CC rich protein (OmcA) and the large cysteine-rich periplasmic protein
CC (OmcB). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: It is present but the disulfide bonds are reduced
CC in the intracellular reticulate bodies (RBs).
CC {ECO:0000269|PubMed:7582008}.
CC -!- CAUTION: Was thought to be an outer membrane protein as it is part of a
CC disulfide cross-linked complex that is insoluble in the detergent
CC Sarkosyl; however based on experiments in C.psittaci it is likely to be
CC periplasmic. {ECO:0000305}.
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DR EMBL; X53511; CAA37590.1; -; Genomic_DNA.
DR EMBL; AE001363; AAD18697.1; -; Genomic_DNA.
DR EMBL; AE002161; AAF38068.1; -; Genomic_DNA.
DR EMBL; BA000008; BAA98763.1; -; Genomic_DNA.
DR EMBL; AE009440; AAP98508.1; -; Genomic_DNA.
DR PIR; A86560; A86560.
DR PIR; S12602; S12602.
DR RefSeq; NP_224753.1; NC_000922.1.
DR RefSeq; WP_010883195.1; NZ_LN847257.1.
DR AlphaFoldDB; P23700; -.
DR STRING; 115711.CP_0195; -.
DR EnsemblBacteria; AAD18697; AAD18697; CPn_0557.
DR EnsemblBacteria; AAF38068; AAF38068; CP_0195.
DR KEGG; cpa:CP_0195; -.
DR KEGG; cpj:omcB; -.
DR KEGG; cpn:CPn_0557; -.
DR KEGG; cpt:CpB0579; -.
DR PATRIC; fig|115713.3.peg.618; -.
DR eggNOG; COG1361; Bacteria.
DR HOGENOM; CLU_029611_0_0_0; -.
DR OrthoDB; 1049286at2; -.
DR Proteomes; UP000000583; Chromosome.
DR Proteomes; UP000000801; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005201; F:extracellular matrix structural constituent; IEA:InterPro.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR InterPro; IPR003506; Chlam_OMP6.
DR InterPro; IPR001434; DUF11.
DR Pfam; PF03504; Chlam_OMP6; 1.
DR Pfam; PF01345; DUF11; 3.
DR PRINTS; PR01336; CHLAMIDIAOM6.
DR TIGRFAMs; TIGR01451; B_ant_repeat; 1.
PE 2: Evidence at transcript level;
KW Cell shape; Disulfide bond; Periplasm; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..40
FT /evidence="ECO:0000255"
FT /id="PRO_0000020164"
FT CHAIN 41..556
FT /note="Large cysteine-rich periplasmic protein OmcB"
FT /id="PRO_0000020165"
FT CONFLICT 412
FT /note="T -> I (in Ref. 6; AAP98508)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 556 AA; 59719 MW; 8D7ED9234CC99458 CRC64;
MSKLIRRVVT VLALTSMASC FASGGIEAAV AESLITKIVA SAETKPAPVP MTAKKVRLVR
RNKQPVEQKS RGAFCDKEFY PCEEGRCQPV EAQQESCYGR LYSVKVNDDC NVEICQSVPE
YATVGSPYPI EILAIGKKDC VDVVITQQLP CEAEFVSSDP ETTPTSDGKL VWKIDRLGAG
DKCKITVWVK PLKEGCCFTA ATVCACPELR SYTKCGQPAI CIKQEGPDCA CLRCPVCYKI
EVVNTGSAIA RNVTVDNPVP DGYSHASGQR VLSFNLGDMR PGDKKVFTVE FCPQRRGQIT
NVATVTYCGG HKCSANVTTV VNEPCVQVNI SGADWSYVCK PVEYSISVSN PGDLVLHDVV
IQDTLPSGVT VLEAPGGEIC CNKVVWRIKE MCPGETLQFK LVVKAQVPGR FTNQVAVTSE
SNCGTCTSCA ETTTHWKGLA ATHMCVLDTN DPICVGENTV YRICVTNRGS AEDTNVSLIL
KFSKELQPIA SSGPTKGTIS GNTVVFDALP KLGSKESVEF SVTLKGIAPG DARGEAILSS
DTLTSPVSDT ENTHVY