ID   OMCB_CHLPS              Reviewed;         557 AA.
AC   P0CZ18; P23701;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Large cysteine-rich periplasmic protein omcB;
DE            Short=Large-CRP;
DE   AltName: Full=60 kDa cysteine-rich OMP;
DE   AltName: Full=60 kDa outer membrane protein;
DE   AltName: Full=Cysteine-rich outer membrane protein;
DE   Contains:
DE     RecName: Full=Large cysteine-rich periplasmic protein omcB-alpha;
DE   Contains:
DE     RecName: Full=Large cysteine-rich periplasmic protein omcB-beta;
DE   Flags: Precursor;
GN   Name=omcB; Synonyms=envB;
OS   Chlamydia psittaci (Chlamydophila psittaci).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=83554;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A22/M;
RX   PubMed=2402464; DOI=10.1093/nar/18.17.5300;
RA   Watson M.W., Lambden P.R., Clarke I.N.;
RT   "The nucleotide sequence of the 60 kDa cysteine rich outer membrane protein
RT   of Chlamydia psittaci strain EAE/A22/M.";
RL   Nucleic Acids Res. 18:5300-5300(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC   STRAIN=A22/M;
RX   PubMed=7582008; DOI=10.1099/13500872-141-10-2489;
RA   Watson M.W., Clarke I.N., Everson J.S., Lambden P.R.;
RT   "The CrP operon of Chlamydia psittaci and Chlamydia pneumoniae.";
RL   Microbiology 141:2489-2497(1995).
CC   -!- FUNCTION: In elementary bodies (EBs, the infectious stage, which is
CC       able to survive outside the host cell) provides the structural
CC       integrity of the outer envelope through disulfide cross-links with the
CC       small cysteine-rich protein and the major outer membrane porin. It has
CC       been described in publications as the Sarkosyl-insoluble COMC
CC       (Chlamydia outer membrane complex), and serves as the functional
CC       equivalent of peptidoglycan (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a disulfide cross-linked outer membrane complex (COMC)
CC       composed of the major outer membrane porin (MOMP), the small cysteine-
CC       rich protein (omcA) and the large cysteine-rich periplasmic protein
CC       (omcB). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: It is present but the disulfide bonds are reduced
CC       in the intracellular reticulate bodies (RBs).
CC       {ECO:0000269|PubMed:7582008}.
CC   -!- CAUTION: Was thought to be an outer membrane protein as it is part of a
CC       disulfide cross-linked complex that is insoluble in the detergent
CC       Sarkosyl. In PubMed:7532170 it was shown to likely be periplasmic.
CC       {ECO:0000305}.
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DR   EMBL; X53512; CAA37592.1; -; Genomic_DNA.
DR   PIR; B39439; B39439.
DR   RefSeq; WP_014947026.1; NZ_JACAAQ010000029.1.
DR   AlphaFoldDB; P0CZ18; -.
DR   STRING; 331636.G5O_0210; -.
DR   eggNOG; COG1361; Bacteria.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005201; F:extracellular matrix structural constituent; IEA:InterPro.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   InterPro; IPR003506; Chlam_OMP6.
DR   InterPro; IPR001434; DUF11.
DR   Pfam; PF03504; Chlam_OMP6; 1.
DR   Pfam; PF01345; DUF11; 2.
DR   PRINTS; PR01336; CHLAMIDIAOM6.
DR   TIGRFAMs; TIGR01451; B_ant_repeat; 1.
PE   2: Evidence at transcript level;
KW   Cell shape; Disulfide bond; Periplasm; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000250"
FT   PROPEP          23..40
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000020166"
FT   CHAIN           41..557
FT                   /note="Large cysteine-rich periplasmic protein omcB-alpha"
FT                   /id="PRO_0000020167"
FT   CHAIN           58..557
FT                   /note="Large cysteine-rich periplasmic protein omcB-beta"
FT                   /id="PRO_0000248870"
SQ   SEQUENCE   557 AA;  59843 MW;  0D444F09EAA073C6 CRC64;
     MSKLIRRVVT VLALTSMASS FASGKIEAAA AESLATRFIA STENADDNVF QATAKKVRFG
     RNKNQRQEQK HTEAFCDKEF YPCEGGQCQP VDATQESCYG KMYCVRVNDD CNVEISQSVP
     EYATVGSPYP IEILAVGKKD CVNVVITQQL PCEVEFVSSD PATTPTSDSK LIWTIDRLGQ
     GEKCKITVWV KPLKEGCCFT AATVCACPEL RSYTKCGQPA ICIKQEGPEC ACLRCPVCYK
     IEVCNTGSAI ARNVVVDNPV PDGYTHASGQ RVLSFNLGDM RPGDSKCFCV EFCPQKRGKV
     TNVATVSYCG GHKCSANVTT VVNEPCVQVN ISGADWSYVC KPVEYTIVVS NPGDLKLYDV
     VIEDTAPSGA TILEAAGAEI CCNKAVWCIK EMCPGETLQF KVVAKAQSPG KFTNQVVVKT
     NSDCGTCTSC AEVTTHWKGL AATHMCVIDT NDPICVGENT VYRICVTNRG SAEDTNVSLI
     LKFSKELQPV SSSGPTKGTI TGNTVVFDAL PKLGSKESVE FSVTLKGIAP GDARGEAILS
     SDTLTVPVAD TENTHVY