OMCB_CHLT2
ID OMCB_CHLT2 Reviewed; 547 AA.
AC B0B815; P18586; P21354;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 2.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Large cysteine-rich periplasmic protein OmcB;
DE Short=Large-CRP;
DE AltName: Full=60 kDa CRP;
DE AltName: Full=60 kDa outer membrane protein;
DE AltName: Full=Cysteine-rich outer membrane protein;
DE Contains:
DE RecName: Full=Large cysteine-rich periplasmic protein OmcB, isoform 1;
DE Contains:
DE RecName: Full=Large cysteine-rich periplasmic protein OmcB, isoform 2;
DE Flags: Precursor;
GN Name=omcB; Synonyms=omp2, omp2B; OrderedLocusNames=CTL0702;
OS Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=471472;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-32 AND 41-51,
RP DEVELOPMENTAL STAGE, AND EXISTENCE OF TWO MATURE FORMS.
RC STRAIN=434/Bu / ATCC VR-902B;
RX PubMed=2914847; DOI=10.1128/jb.171.1.285-291.1989;
RA Allen J.E., Stephens R.S.;
RT "Identification by sequence analysis of two-site posttranslational
RT processing of the cysteine-rich outer membrane protein 2 of Chlamydia
RT trachomatis serovar L2.";
RL J. Bacteriol. 171:285-291(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=434/Bu / ATCC VR-902B;
RX PubMed=18032721; DOI=10.1101/gr.7020108;
RA Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P.,
RA Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D.,
RA Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N.;
RT "Chlamydia trachomatis: genome sequence analysis of lymphogranuloma
RT venereum isolates.";
RL Genome Res. 18:161-171(2008).
CC -!- FUNCTION: In elementary bodies (EBs, the infectious stage, which is
CC able to survive outside the host cell) provides the structural
CC integrity of the outer envelope through disulfide cross-links with the
CC small cysteine-rich protein and the major outer membrane protein. It
CC has been described in publications as the Sarkosyl-insoluble COMC
CC (Chlamydia outer membrane complex), and serves as the functional
CC equivalent of peptidoglycan.
CC -!- SUBUNIT: Part of a disulfide cross-linked outer membrane complex (COMC)
CC composed of the major outer membrane porin (MOMP), the small cysteine-
CC rich protein (OmcA) and the large cysteine-rich periplasmic protein
CC (OmcB).
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: It is present but the disulfide bonds are reduced
CC in the intracellular reticulate bodies (RBs).
CC {ECO:0000269|PubMed:2914847}.
CC -!- MISCELLANEOUS: In strain L2 the origin of the observed doublet has been
CC shown to be different post-translational cleavage.
CC -!- CAUTION: Was thought to be an outer membrane protein as it is part of a
CC disulfide cross-linked complex that is insoluble in the detergent
CC Sarkosyl; however based on experiments in C.psittaci it is likely to be
CC periplasmic. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAP04141.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M23001; AAA23152.1; -; Genomic_DNA.
DR EMBL; AM884176; CAP04141.1; ALT_INIT; Genomic_DNA.
DR PIR; A32244; A32244.
DR RefSeq; WP_013984953.1; NC_010287.1.
DR RefSeq; YP_001654774.1; NC_010287.1.
DR AlphaFoldDB; B0B815; -.
DR SMR; B0B815; -.
DR TCDB; 1.B.2.1.2; the chlamydial porin (cp) family.
DR EnsemblBacteria; CAP04141; CAP04141; CTL0702.
DR KEGG; ctb:CTL0702; -.
DR PATRIC; fig|471472.4.peg.754; -.
DR HOGENOM; CLU_029611_0_0_0; -.
DR Proteomes; UP000000795; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005201; F:extracellular matrix structural constituent; IEA:InterPro.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR003506; Chlam_OMP6.
DR InterPro; IPR001434; DUF11.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF03504; Chlam_OMP6; 1.
DR Pfam; PF01345; DUF11; 3.
DR PRINTS; PR01336; CHLAMIDIAOM6.
DR TIGRFAMs; TIGR01451; B_ant_repeat; 2.
PE 1: Evidence at protein level;
KW Cell shape; Direct protein sequencing; Disulfide bond; Periplasm; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:2914847"
FT CHAIN 23..547
FT /note="Large cysteine-rich periplasmic protein OmcB,
FT isoform 1"
FT /id="PRO_0000417577"
FT CHAIN 41..547
FT /note="Large cysteine-rich periplasmic protein OmcB,
FT isoform 2"
FT /id="PRO_0000417578"
FT REGION 45..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..65
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 547 AA; 58782 MW; 78CEB41CCF98472D CRC64;
MNKLIRRAVT IFAVTSVASL FASGVLETSM AEFISTNVIS LADTKAKDNT SHKSKKARKN
HSKETPVNRK KVAPVHESKA TGPKQDSCFG RMYTVKVNDD RNVEITQAVP KYATVGSPYP
VEITATGKRD CVDVIITQQL PCEAEFVRSD PATTPTADGK LVWKIDRLGQ GEKSKITVWV
KPLKEGCCFT AATVCACPEI RSVTKCGQPA ICVKQEGPEN ACLRCPVVYK INVVNQGTAT
ARNVVVENPV PDSYAHSSGQ RVLTFTLGDM QPGEHRTITV EFCPLKRGRA TNIAMVSYCG
GHKNTASVTT VINEPCVQVS IAGADWSYVC KPVEYVISVS NPGDLVLRDV VVKDTLSPGV
TVLEAAGAQI SCNKVVWTVK ELNPGESLQY KVLVRAQTPG QFTNNVVVKS CSDCGTCTSC
AEATTYWKGV AATHMCVVDT CDPVCVGENT VYRICVTNRG SAEDTNVSLM LKFSKELQPV
SFSGPTKGTI TGNTVVFDSL PRLGSKETVE FSVTLKAVSA GDARGEAILS SDTLTVPVSD
TENTHIY
