ID   OMH4_SCHPO              Reviewed;         440 AA.
AC   O94565;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 2.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=O-glycoside alpha-1,2-mannosyltransferase homolog 4;
DE            EC=2.4.1.-;
GN   Name=omh4; ORFNames=SPBC1773.08c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   REVISION OF GENE MODEL.
RX   PubMed=21511999; DOI=10.1126/science.1203357;
RA   Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA   Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA   Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA   Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA   French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA   Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA   Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA   Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA   Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA   Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT   "Comparative functional genomics of the fission yeasts.";
RL   Science 332:930-936(2011).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=19054127; DOI=10.1111/j.1567-1364.2008.00458.x;
RA   Ikeda Y., Ohashi T., Tanaka N., Takegawa K.;
RT   "Identification and characterization of a gene required for alpha1,2-
RT   mannose extension in the O-linked glycan synthesis pathway in
RT   Schizosaccharomyces pombe.";
RL   FEMS Yeast Res. 9:115-125(2009).
CC   -!- FUNCTION: Probable mannosyltransferase involved in O-glycosylation of
CC       cell wall and secreted proteins. Transfers an alpha-D-mannosyl residue
CC       from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-
CC       (1->2)-D-mannosyl-D-mannose linkage. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC       {ECO:0000269|PubMed:16823372}. Golgi apparatus membrane {ECO:0000305};
CC       Single-pass type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 15 family.
CC       {ECO:0000305}.
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DR   EMBL; CU329671; CAA21913.2; -; Genomic_DNA.
DR   PIR; T39673; T39673.
DR   RefSeq; NP_595123.2; NM_001021030.2.
DR   AlphaFoldDB; O94565; -.
DR   SMR; O94565; -.
DR   BioGRID; 276712; 2.
DR   STRING; 4896.SPBC1773.08c.1; -.
DR   CAZy; GT15; Glycosyltransferase Family 15.
DR   PaxDb; O94565; -.
DR   EnsemblFungi; SPBC1773.08c.1; SPBC1773.08c.1:pep; SPBC1773.08c.
DR   GeneID; 2540179; -.
DR   KEGG; spo:SPBC1773.08c; -.
DR   PomBase; SPBC1773.08c; omh4.
DR   VEuPathDB; FungiDB:SPBC1773.08c; -.
DR   eggNOG; KOG4472; Eukaryota.
DR   HOGENOM; CLU_024327_2_1_1; -.
DR   InParanoid; O94565; -.
DR   OMA; HDKVFVT; -.
DR   PRO; PR:O94565; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; ISS:PomBase.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; ISM:PomBase.
DR   GO; GO:0000032; P:cell wall mannoprotein biosynthetic process; ISM:PomBase.
DR   GO; GO:0097502; P:mannosylation; IBA:GO_Central.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR002685; Glyco_trans_15.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR31121; PTHR31121; 2.
DR   Pfam; PF01793; Glyco_transf_15; 1.
DR   PIRSF; PIRSF018153; Glyco_trans_15; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycosyltransferase; Golgi apparatus; Membrane; Nucleus;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..440
FT                   /note="O-glycoside alpha-1,2-mannosyltransferase homolog 4"
FT                   /id="PRO_0000316586"
FT   TOPO_DOM        1..35
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        57..440
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        336
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   440 AA;  51375 MW;  15BF935CB83652C6 CRC64;
     MLGWNKHVFF SESRINFRCL LRKKLKKRCP LSARFVLVLL LIVLIFILKM GYKQLIYKLN
     HPPLRRIDER NDPLFSNGCK NVHLEAQLHP RMNATFVMLA RNSDLPGVVS SINSLEKHFN
     RHFNYPYTFL NDEPFDEKFK ETILKLTSAN VEFGTLEKDT FGFPGNVDVD AAREAIASQG
     DRGIMYGDTE SYHHMCRFFS GFFYKHPLLL KYQWYWRVEP DVAFTCDISY DPFYYMEENG
     KIYGYVVALK ELEDTVPNLF RYTSAYRRNN NLTSNMWKFF LDAPKKENYD ISRKDPTVGL
     SFSSHLNAMI DSSYSAETSS MEGESYNMCH FWSNFEIANF KFFRNEQYEN FFRTMDATGG
     FWTERWGDAP FHSLAAGLFL SKEQVHYFRD LGYRHSDIHH CGQSLGCNCE CIPELSEIES
     TSGGCVTQWV NLIGDGFLDE