ID   OMP2A_BRUME             Reviewed;         367 AA.
AC   Q7CNU3; Q45312;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Porin Omp2a;
DE   Flags: Precursor;
GN   Name=omp2a; OrderedLocusNames=BMEI1306;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=biovar 1;
RX   PubMed=8573514; DOI=10.1099/00207713-46-1-329;
RA   Ficht T.A., Husseinen H.S., Derr J., Bearden S.W.;
RT   "Species-specific sequences at the omp2 locus of Brucella type strains.";
RL   Int. J. Syst. Bacteriol. 46:329-331(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
RN   [3]
RP   FUNCTION IN PERMEABILITY TO SUGAR.
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11466287; DOI=10.1128/jb.183.16.4839-4847.2001;
RA   Paquet J.-Y., Diaz M.A., Genevrois S., Grayon M., Verger J.-M.,
RA   de Bolle X., Lakey J.H., Letesson J.-J., Cloeckaert A.;
RT   "Molecular, antigenic, and functional analyses of Omp2b porin size variants
RT   of Brucella spp.";
RL   J. Bacteriol. 183:4839-4847(2001).
CC   -!- FUNCTION: Forms passive diffusion pores that allow small molecular
CC       weight hydrophilic materials across the outer membrane.
CC       {ECO:0000269|PubMed:11466287}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:B2SAB9}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000250|UniProtKB:B2SAB9}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:B2SAB9}.
CC   -!- DOMAIN: Consists of 16-stranded beta-barrel sheets, with large surface-
CC       exposed loops, that form a transmembrane pore at the center of each
CC       barrel. The pore is partially ocluded by a peptide loop that folds into
CC       the pore lumen. {ECO:0000250|UniProtKB:B2SAB9}.
CC   -!- MISCELLANEOUS: The pore formed by Omp2a is larger than the one formed
CC       by Omp2b. Omp2b pores have optimal permeability to allow growth and
CC       protection against harmful compounds. The larger pore formed by Omp2a
CC       may be advantageous for intracellular growth, when the bacterium is
CC       competing with the host cell for nutrients whose concentration is
CC       particularly low within the phagosome. {ECO:0000250|UniProtKB:B2SAB9}.
CC   -!- SIMILARITY: Belongs to the alphaproteobacteria porin family.
CC       {ECO:0000305}.
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DR   EMBL; U26440; AAA67789.1; -; Genomic_DNA.
DR   EMBL; AE008917; AAL52487.1; -; Genomic_DNA.
DR   RefSeq; WP_002970988.1; NZ_GG703778.1.
DR   AlphaFoldDB; Q7CNU3; -.
DR   STRING; 224914.BMEI1306; -.
DR   EnsemblBacteria; AAL52487; AAL52487; BMEI1306.
DR   GeneID; 29594164; -.
DR   KEGG; bme:BMEI1306; -.
DR   PATRIC; fig|224914.52.peg.102; -.
DR   eggNOG; COG3203; Bacteria.
DR   OMA; NMRFTLR; -.
DR   PhylomeDB; Q7CNU3; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR003684; Porin_alphabac.
DR   Pfam; PF02530; Porin_2; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Ion transport; Membrane; Porin; Signal; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..367
FT                   /note="Porin Omp2a"
FT                   /id="PRO_0000354013"
FT   VARIANT         301
FT                   /note="P -> T (in strain: biovar 1)"
FT   VARIANT         362
FT                   /note="R -> P (in strain: biovar 1)"
SQ   SEQUENCE   367 AA;  39478 MW;  8730BF084D70FD5F CRC64;
     MNIKSLLLGS AAALVAASGA QAADAIVAPE PEAVEYVRVC DAYGAGYFYI PGTETCLRVH
     GYVRYDVKGG DDVYSGTDRN GWDKGARFAL MFNTNSETEL GTLGTYTQLR FNYTSNNSRH
     DGQYGDFSDD RDVADGGVST GTDLQFAYIT LGGFKVGIDE SEFHTFTGYL GDVINDDVVA
     AGSYRTGKIA YTFTGGNGFS AVIALEQGGE DVDNDYTIDG YMPHVVGGLK YAGGWGSIAG
     VVAYDSVIEE WATKVRGDVN ITDRFSVWLQ GAYSSAATPN QNYGQWGGDW AVWGGAKFIA
     PEKATFNLQA AHDDWGKTAV TANVAYQLVP GFTITPEVSY TKFGGEWKDT VAEDNAWGGI
     VRFQRSF