OMP2B_BRUSS
ID OMP2B_BRUSS Reviewed; 375 AA.
AC P0DI96; Q2KMJ8; Q2KMJ9; Q2KMK0; Q2KMK1; Q2KMK2; Q2KMK3; Q2KMK4; Q45428;
AC Q7CEH8; Q9KH74;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=Porin Omp2b;
DE Flags: Precursor;
GN Name=omp2b;
OS Brucella suis.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=29461;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN PERMEABILITY TO SUGAR.
RC STRAIN=83-210;
RX PubMed=11466287; DOI=10.1128/jb.183.16.4839-4847.2001;
RA Paquet J.-Y., Diaz M.A., Genevrois S., Grayon M., Verger J.-M.,
RA de Bolle X., Lakey J.H., Letesson J.-J., Cloeckaert A.;
RT "Molecular, antigenic, and functional analyses of Omp2b porin size variants
RT of Brucella spp.";
RL J. Bacteriol. 183:4839-4847(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-375.
RC STRAIN=686, De12, Fr11, Fr7, Pt1, Thomsen, and Wf21;
RX PubMed=16530357; DOI=10.1016/j.vetmic.2006.02.002;
RA Ferrao-Beck L., Cardoso R., Munoz P.M., de Miguel M.J., Albert D.,
RA Ferreira A.C., Marin C.M., Thiebaud M., Jacques I., Grayon M.,
RA Zygmunt M.S., Garin-Bastuji B., Blasco J.M., Sa M.I.;
RT "Development of a multiplex PCR assay for polymorphism analysis of Brucella
RT suis biovars causing brucellosis in swine.";
RL Vet. Microbiol. 115:269-277(2006).
CC -!- FUNCTION: Forms passive diffusion pores that allow small molecular
CC weight hydrophilic materials across the outer membrane.
CC {ECO:0000269|PubMed:11466287}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250|UniProtKB:Q44665}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000250|UniProtKB:Q44665}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q44665}.
CC -!- DOMAIN: Consists of 16-stranded beta-barrel sheets, with large surface-
CC exposed loops, that form a transmembrane pore at the center of each
CC barrel. The pore is partially ocluded by a peptide loop that folds into
CC the pore lumen. {ECO:0000250|UniProtKB:Q44665}.
CC -!- MISCELLANEOUS: The pore formed by Omp2a is larger than the one formed
CC by Omp2b. Omp2b pores have optimal permeability to allow growth and
CC protection against harmful compounds. The larger pore formed by Omp2a
CC may be advantageous for intracellular growth, when the bacterium is
CC competing with the host cell for nutrients whose concentration is
CC particularly low within the phagosome. {ECO:0000250|UniProtKB:Q44665}.
CC -!- SIMILARITY: Belongs to the alphaproteobacteria porin family.
CC {ECO:0000305}.
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DR EMBL; AF268034; AAF80105.1; -; Genomic_DNA.
DR EMBL; AY903259; AAX83995.1; -; Genomic_DNA.
DR EMBL; AY903260; AAX83996.1; -; Genomic_DNA.
DR EMBL; AY903261; AAX83997.1; -; Genomic_DNA.
DR EMBL; AY903262; AAX83998.1; -; Genomic_DNA.
DR EMBL; AY903263; AAX83999.1; -; Genomic_DNA.
DR EMBL; AY903264; AAX84000.1; -; Genomic_DNA.
DR EMBL; AY903265; AAX84001.1; -; Genomic_DNA.
DR AlphaFoldDB; P0DI96; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR003684; Porin_alphabac.
DR Pfam; PF02530; Porin_2; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Ion transport; Membrane; Porin; Signal; Transmembrane;
KW Transmembrane beta strand; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..375
FT /note="Porin Omp2b"
FT /id="PRO_0000412629"
FT VARIANT 59..60
FT /note="VH -> IS (in strain: Fr7)"
FT VARIANT 75..77
FT /note="SGT -> TGS (in strain: 686, De12, Fr7, Pt1 and
FT Thomsen)"
FT VARIANT 80
FT /note="K -> N (in strain: Fr11 and Wf21)"
FT VARIANT 85
FT /note="S -> G (in strain: De12, Fr7, Pt1 and Thomsen)"
FT VARIANT 91..95
FT /note="RVSTG -> MFNTN (in strain: De12, Fr7, Pt1 and
FT Thomsen)"
FT VARIANT 114..129
FT /note="SASNSREDGYYGTNSD -> AANNSGVDGKYGNETSS (in strain:
FT 686, De12, Fr7, Fr11, Pt1, Thomsen and Wf21)"
FT VARIANT 134
FT /note="Q -> E (in strain: 686, De12, Fr7, Fr11, Pt1,
FT Thomsen and Wf21)"
FT VARIANT 153
FT /note="Q -> H (in strain: 686, De12, Fr7, Fr11, Pt1,
FT Thomsen and Wf21)"
FT VARIANT 172
FT /note="T -> S (in strain: 686, De12, Fr7, Fr11, Pt1,
FT Thomsen and Wf21)"
FT VARIANT 206..222
FT /note="PVFKDSQGREINGRGYQ -> GTTNYH (in strain: 686, De12,
FT Fr7, Fr11, Pt1, Thomsen and Wf21)"
FT VARIANT 258
FT /note="T -> A (in strain: 686, De12, Fr7, Fr11, Pt1,
FT Thomsen and Wf21)"
FT VARIANT 339
FT /note="I -> V (in strain: 686, De12, Fr7, Fr11, Pt1,
FT Thomsen and Wf21)"
FT VARIANT 349..350
FT /note="SN -> GG (in strain: 686, De12, Fr7, Fr11, Pt1,
FT Thomsen and Wf21)"
FT VARIANT 354..363
FT /note="RELGNDTLDD -> NTVAEDN (in strain: 686, De12, Fr7,
FT Fr11, Pt1, Thomsen and Wf21)"
SQ SEQUENCE 375 AA; 40569 MW; 539EB73D9B828DBB CRC64;
MNIKSLLLGS AAALVAASGA QAADAIVAPE PEAVEYVRVC DAYGAGYFYI PGTETCLRVH
GYVRYDVKGG DDVYSGTDRK GWDKSARFAL RVSTGSETEL GTLKTFTELR FNYSASNSRE
DGYYGTNSDG TVMQFAYIQL GGLRVGIDES EFQTFTGYLG DVINDDVISA GTYRTGKISY
TFTGGNGFSA VIALEQGGDN DGGYTPVFKD SQGREINGRG YQIDGYMPDV VGGLKYAGGW
GSIAGVVAYD SVIEEWATKV RGDVNITDQF SVWLQGAYSS AATPDQNYGQ WGGDWAVWGG
LKYQATQKAA FNLQAAHDDW GKTAVTANVA YELVPGFTIT PEVSYTKFSN EWKRELGNDT
LDDAWGGIVR FQRSF
