OMPA_KLEAE
ID OMPA_KLEAE Reviewed; 350 AA.
AC P09146;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Outer membrane protein A {ECO:0000255|HAMAP-Rule:MF_00842};
DE AltName: Full=Outer membrane porin A {ECO:0000255|HAMAP-Rule:MF_00842};
DE Flags: Precursor;
GN Name=ompA {ECO:0000255|HAMAP-Rule:MF_00842};
OS Klebsiella aerogenes (Enterobacter aerogenes).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=548;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6363059; DOI=10.1111/j.1432-1033.1983.tb07853.x;
RA Braun G., Cole S.T.;
RT "Molecular characterization of the gene coding for major outer membrane
RT protein OmpA from Enterobacter aerogenes.";
RL Eur. J. Biochem. 137:495-500(1983).
CC -!- FUNCTION: With TolR probably plays a role in maintaining the position
CC of the peptidoglycan cell wall in the periplasm. Acts as a porin with
CC low permeability that allows slow penetration of small solutes; an
CC internal gate slows down solute passage. {ECO:0000255|HAMAP-
CC Rule:MF_00842}.
CC -!- FUNCTION: Required for conjugation with F-type plasmids; probably
CC serves as the mating receptor on recipient cells. {ECO:0000255|HAMAP-
CC Rule:MF_00842}.
CC -!- SUBUNIT: Monomer and homodimer. {ECO:0000255|HAMAP-Rule:MF_00842}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC Rule:MF_00842}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00842}.
CC -!- DOMAIN: The extracellular loops are most variable in sequence, and in
CC some bacteria confer sensitivity to phage and/or colicins.
CC {ECO:0000255|HAMAP-Rule:MF_00842}.
CC -!- SIMILARITY: Belongs to the outer membrane OOP (TC 1.B.6) superfamily.
CC OmpA family. {ECO:0000255|HAMAP-Rule:MF_00842}.
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DR EMBL; X00254; CAA25062.1; -; Genomic_DNA.
DR PIR; S07222; S07222.
DR AlphaFoldDB; P09146; -.
DR SMR; P09146; -.
DR STRING; 548.EAG7_02416; -.
DR PRIDE; P09146; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015288; F:porin activity; IEA:UniProtKB-UniRule.
DR GO; GO:0034220; P:ion transmembrane transport; IEA:UniProtKB-UniRule.
DR CDD; cd07185; OmpA_C-like; 1.
DR Gene3D; 3.30.1330.60; -; 1.
DR HAMAP; MF_00842; OmpA; 1.
DR InterPro; IPR011250; OMP/PagP_b-brl.
DR InterPro; IPR006664; OMP_bac.
DR InterPro; IPR002368; OmpA.
DR InterPro; IPR006665; OmpA-like.
DR InterPro; IPR006690; OMPA-like_CS.
DR InterPro; IPR036737; OmpA-like_sf.
DR InterPro; IPR000498; OmpA-like_TM_dom.
DR Pfam; PF00691; OmpA; 1.
DR Pfam; PF01389; OmpA_membrane; 1.
DR PRINTS; PR01021; OMPADOMAIN.
DR PRINTS; PR01022; OUTRMMBRANEA.
DR SUPFAM; SSF103088; SSF103088; 1.
DR SUPFAM; SSF56925; SSF56925; 1.
DR PROSITE; PS01068; OMPA_1; 1.
DR PROSITE; PS51123; OMPA_2; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Conjugation; Disulfide bond; Ion transport; Membrane;
KW Porin; Repeat; Signal; Transmembrane; Transmembrane beta strand; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT CHAIN 22..350
FT /note="Outer membrane protein A"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT /id="PRO_0000020096"
FT TRANSMEM 27..37
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 59..70
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 74..82
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 100..111
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 116..124
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 146..155
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 160..167
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT TRANSMEM 186..194
FT /note="Beta stranded"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT REPEAT 205..206
FT /note="1"
FT REPEAT 207..208
FT /note="2"
FT REPEAT 209..210
FT /note="3"
FT REPEAT 211..212
FT /note="4"
FT DOMAIN 214..342
FT /note="OmpA-like"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT REGION 205..212
FT /note="4 X 2 AA tandem repeats of A-P"
FT SITE 77
FT /note="Part of salt bridge gating mechanism"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT SITE 163
FT /note="Part of salt bridge gating mechanism"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
FT DISULFID 315..327
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842"
SQ SEQUENCE 350 AA; 37575 MW; 6276C6F2F21065DA CRC64;
MKKTAIAIAV ALAGFATVAQ AAPKDNTWYA GGKLGWSQFH DTGWYNSNLN NNGPTHESQL
GAGAFGGYQV NPYLGFEMGY DWLGRMPYKG VKVNGAFSSQ AVQLTAKLGY PITDDLDIYT
RLGGMVWRAD SSNSIAGDNH DTGVSPVFAG GVEWAMTRDI ATRLEYQWVN NIGDAGTVGV
RPDNGMLSVG VSYRFGQEDN APVVAPAPAP APEVTTKTFT LKSDVLFNFN KATLKPEGQQ
ALDQLYTQLS NMDPKDGSAV VLGYTDRIGS EQYNQKLSEK RAQSVVDYLV AKGIPANKIS
ARGMGESDPV TGNTCDNVKA RAALIDCLAP DRRVAIEVKG YKDVVTQPQA
