ID   OMPA_NEIMB              Reviewed;         392 AA.
AC   P0DH58; Q51240;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Major outer membrane protein P.IA;
DE            Short=PIA;
DE            Short=Protein IA;
DE   AltName: Full=Class 1 protein;
DE   Flags: Precursor;
GN   Name=porA; OrderedLocusNames=NMB1429;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710308; DOI=10.1126/science.287.5459.1816;
RA   Pizza M., Scarlato V., Masignani V., Giuliani M.M., Arico' B.,
RA   Comanducci M., Jennings G.T., Baldi L., Bartolini E., Capecchi B.,
RA   Galeotti C.L., Luzzi E., Manetti R., Marchetti E., Mora M., Nuti S.,
RA   Ratti G., Santini L., Savino S., Scarselli M., Storni E., Zuo P.,
RA   Broeker M., Hundt E., Knapp B., Blair E., Mason T., Tettelin H., Hood D.W.,
RA   Jeffries A.C., Saunders N.J., Granoff D.M., Venter J.C., Moxon E.R.,
RA   Grandi G., Rappuoli R.;
RT   "Identification of vaccine candidates against serogroup B meningococcus by
RT   whole-genome sequencing.";
RL   Science 287:1816-1820(2000).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17038831; DOI=10.4161/hv.1.2.1651;
RA   Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J.;
RT   "Characterization of the protein content of a meningococcal outer membrane
RT   vesicle vaccine by polyacrylamide gel electrophoresis and mass
RT   spectrometry.";
RL   Hum. Vaccin. 1:80-84(2005).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=NZ98/254 / Serogroup B;
RX   PubMed=16645985; DOI=10.1002/pmic.200500821;
RA   Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.;
RT   "Proteomic analysis of a meningococcal outer membrane vesicle vaccine
RT   prepared from the group B strain NZ98/254.";
RL   Proteomics 6:3400-3413(2006).
CC   -!- FUNCTION: Serves as a slightly cation selective porin. Major antigen on
CC       the gonococcal cell surface and it may have pathogenic properties in
CC       addition to its porin activity.
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein.
CC   -!- MISCELLANEOUS: Present in outer membrane vesicle formulations which are
CC       used as vaccines in human.
CC   -!- SIMILARITY: Belongs to the Gram-negative porin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE002098; AAF41790.1; -; Genomic_DNA.
DR   EMBL; AF226344; AAF42493.1; -; Genomic_DNA.
DR   PIR; F81084; F81084.
DR   RefSeq; NP_274441.1; NC_003112.2.
DR   RefSeq; WP_010980936.1; NC_003112.2.
DR   AlphaFoldDB; P0DH58; -.
DR   SMR; P0DH58; -.
DR   STRING; 122586.NMB1429; -.
DR   PaxDb; P0DH58; -.
DR   ABCD; P0DH58; 2 sequenced antibodies.
DR   EnsemblBacteria; AAF41790; AAF41790; NMB1429.
DR   KEGG; nme:NMB1429; -.
DR   PATRIC; fig|122586.8.peg.1791; -.
DR   HOGENOM; CLU_038238_4_0_4; -.
DR   OMA; GDKTKNS; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0034220; P:ion transmembrane transport; IEA:InterPro.
DR   CDD; cd00342; gram_neg_porins; 1.
DR   Gene3D; 2.40.160.10; -; 1.
DR   InterPro; IPR033900; Gram_neg_porin_domain.
DR   InterPro; IPR023614; Porin_dom_sf.
DR   InterPro; IPR001702; Porin_Gram-ve.
DR   InterPro; IPR013793; Porin_Gram-ve_CS.
DR   InterPro; IPR002299; Porin_Neis.
DR   Pfam; PF00267; Porin_1; 1.
DR   PRINTS; PR00182; ECOLNEIPORIN.
DR   PRINTS; PR00184; NEISSPPORIN.
DR   PROSITE; PS00576; GRAM_NEG_PORIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Ion transport; Membrane; Porin; Reference proteome;
KW   Signal; Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000250"
FT   CHAIN           20..392
FT                   /note="Major outer membrane protein P.IA"
FT                   /id="PRO_0000025274"
SQ   SEQUENCE   392 AA;  42078 MW;  1D78EA55BF76E940 CRC64;
     MRKKLTALVL SALPLAAVAD VSLYGEIKAG VEGRNYQLQL TEAQAANGGA SGQVKVTKVT
     KAKSRIRTKI SDFGSFIGFK GSEDLGDGLK AVWQLEQDVS VAGGGATQWG NRESFIGLAG
     EFGTLRAGRV ANQFDDASQA IDPWDSNNDV ASQLGIFKRH DDMPVSVRYD SPEFSGFSGS
     VQFVPIQNSK SAYTPAYYTK NTNNNLTLVP AVVGKPGSDV YYAGLNYKNG GFAGNYAFKY
     ARHANVGRNA FELFLIGSGS DQAKGTDPLK NHQVHRLTGG YEEGGLNLAL AAQLDLSENG
     DKTKNSTTEI AATASYRFGN AVPRISYAHG FDFIERGKKG ENTSYDQIIA GVDYDFSKRT
     SAIVSGAWLK RNTGIGNYTQ INAASVGLRH KF