ID   OMPA_RICRI              Reviewed;        2249 AA.
AC   P15921;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Outer membrane protein A;
DE   AltName: Full=190 kDa antigen;
DE   AltName: Full=Cell surface antigen;
DE   AltName: Full=rOmp A;
DE            Short=rOmpA;
DE   Contains:
DE     RecName: Full=120 kDa surface-exposed protein;
DE     AltName: Full=120 kDa outer membrane protein OmpA;
DE   Contains:
DE     RecName: Full=32 kDa beta peptide;
DE   Flags: Precursor;
GN   Name=ompA;
OS   Rickettsia rickettsii.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=783;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=R;
RX   PubMed=2117568; DOI=10.1128/iai.58.9.2760-2769.1990;
RA   Anderson B.E., McDonald G.A., Jones D.C., Regnery R.L.;
RT   "A protective protein antigen of Rickettsia rickettsii has tandemly
RT   repeated, near-identical sequences.";
RL   Infect. Immun. 58:2760-2769(1990).
CC   -!- FUNCTION: Elicits protective immunity.
CC   -!- SUBCELLULAR LOCATION: [Outer membrane protein A]: Periplasm
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [120 kDa surface-exposed protein]: Secreted
CC       {ECO:0000250}. Cell surface {ECO:0000250}. Note=Surface exposed. This
CC       bacterium is covered by a S-layer with hexagonal symmetry.
CC   -!- SUBCELLULAR LOCATION: [32 kDa beta peptide]: Cell outer membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=The
CC       cleaved C-terminal fragment (autotransporter domain) is localized in
CC       the outer membrane.
CC   -!- PTM: Glycosylated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the rickettsiae OmpA/OmpB family. {ECO:0000305}.
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DR   EMBL; M31227; AAA26380.1; -; Genomic_DNA.
DR   PIR; A41477; A41477.
DR   AlphaFoldDB; P15921; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.128.130; -; 1.
DR   InterPro; IPR005546; Autotransporte_beta.
DR   InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR   InterPro; IPR006315; OM_autotransptr_brl.
DR   Pfam; PF03797; Autotransporter; 1.
DR   SMART; SM00869; Autotransporter; 1.
DR   SUPFAM; SSF103515; SSF103515; 1.
DR   TIGRFAMs; TIGR01414; autotrans_barl; 1.
DR   PROSITE; PS51208; AUTOTRANSPORTER; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Glycoprotein; Membrane; Periplasm; Repeat; Secreted;
KW   Signal; Transmembrane; Transmembrane beta strand.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..2249
FT                   /note="Outer membrane protein A"
FT                   /id="PRO_0000032647"
FT   CHAIN           29..?
FT                   /note="120 kDa surface-exposed protein"
FT                   /id="PRO_0000387577"
FT   CHAIN           ?..2249
FT                   /note="32 kDa beta peptide"
FT                   /id="PRO_0000387578"
FT   REPEAT          212..286
FT                   /note="Type I 1"
FT   REPEAT          287..358
FT                   /note="Type II 1"
FT   REPEAT          359..430
FT                   /note="Type II 2"
FT   REPEAT          431..505
FT                   /note="Type I 2"
FT   REPEAT          506..577
FT                   /note="Type II 3"
FT   REPEAT          578..652
FT                   /note="Type I 3"
FT   REPEAT          653..724
FT                   /note="Type II 4"
FT   REPEAT          725..799
FT                   /note="Type I 4"
FT   REPEAT          800..874
FT                   /note="Type I 5"
FT   REPEAT          875..949
FT                   /note="Type I 6"
FT   REPEAT          950..1021
FT                   /note="Type II 5"
FT   REPEAT          1022..1093
FT                   /note="Type II 6"
FT   REPEAT          1094..1165
FT                   /note="Type II 7"
FT   REPEAT          1166..1180
FT                   /note="Type I 7; truncated"
FT   DOMAIN          1962..2249
FT                   /note="Autotransporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT   REGION          212..1180
FT                   /note="14 X approximate tandem repeats"
SQ   SEQUENCE   2249 AA;  224333 MW;  A9D6646C089DF087 CRC64;
     MANISPKLFK KAIQQGLKAA LFTTSTAAIM LSSSGALGVA TGVIATNNNA AFSNNVGNNN
     WNEITAAGVA NGTPAGGPQN NWAFTYGGDY TVTADAADRI IKAINVAGTT PVGLNITQNT
     VVGSIITKGN LLPVTLNAGK SLTLNGNNAV AANHGFDAPA DNYTGLGNIA LGGANAALII
     QSAAPSKITL AGNIDGGGII TVKTDAAING TIGNTNALAT VNVGAGTATL GGAVIKATTT
     KLTNAASVLT LTNANAVLTG AIDNTTGGDN VGVLNLNGAL SQVTGDIGNT NSLATISVGA
     GTATLGGAVI KATTTKLTDA ASAVKFTNPV VVTGAIDNTG NANNGIVTFT GNSTVTGNVG
     NTNALATVNV GAGLLQVQGG VVKANTINLT DNASAVTFTN PVVVTGAIDN TGNANNGIVT
     FTGNSTVTGD IGNTNALATV NVGAGTATLG GAVIKATTTK LTNAASVLTL TNANAVLTGA
     IDNTTGGDNV GVLNLNGALS QVTGNIGNTN SLATISVGAG TATLGGAVIK ATTTKLTDAA
     SAVKFTNPVV VTGAIDNTGN ANNGIVTFTG NSTVTGDIGN TNSLATISVG AGTATLGGAV
     IKATTTKLTN AASVLTLTNA NAVLTGAIDN TTGGDNVGVL NLNGALSQVT GDIGNTNSLA
     TISVGAGTAT LGGAVIKATT TKITNAVSAV KFTNPVVVTG AIDSTGNANN GIVTFTGNST
     VTGDIGNTNA LATVNVGAGT ATLGGAVIKA TTTKLTNAAS VLTLTNANAV LTGAIDNTTG
     GDNVGVLNLN GALSQVTGDI GNTNSLATIS VGAGTATLGG AVIKATTTKL TNAASVLTLT
     NANAVLTGAV DNTTGGDNVG VLNLNGALSQ VTGDIGNTNS LATISVGAGT ATLGGAVIKA
     TTTKLTNAAS VLTLTNANAV LTGAIDNTTG GDNVGVLNLN GALSQVTGDI GNTNSLATIS
     VGAGTATLGG AVIKATTTKL TDAASAVKFT NPVVVTGAID NTGNANNGIV TFTGNSTVTG
     NVGNTNALAT VNVGAGLLQV QGGVVKANTI NLTDNASAVT FTNPVVVTGA IDNTGNANNG
     IVTFTGNSTV TGNVGNTNAL ATVNVGAGLL QVQGGVVKAN TINLTDNASA VTFTNPVVVT
     GAIDNTGNAN NGIVTFTGNS TVTGDIGNTN ALATVNVGAG ITLQAGGSLA ANNIDFGARS
     TLEFNGPLDG GGKAIPYYFK GAIANGNNAI LNVNTKLLTA SHLTIGTVAE INIGAGNLFT
     IDASVGDVTI LNAQNINFRA RDSVLVLSNL TGVGVNNILL AADLVAPGAD EGTVVFNGGV
     NGLNVGSNVA GTARNIGDGG GNKFNTLLIY NAVTITDDVN LEGIQNVLIN KNADFTSSTA
     FNAGAIQIND ATYTIDANNG NLNIPAGNIQ FAHADAQLVL QNSSGNDRTI TLGANIDPDN
     DDEGIVILNS VTAGKKLTIA GGKTFGGAHK LQTILFKGAG DCSTAGTTFN TTNIVLDITG
     QLELGATTAN VVLFNDAVQL TQTGNIGGFL DFNAKNGMVT LNNNVNVAGA VQNTGGTNNG
     TLIVLGASNL NRVNGIAMLK VGAGNVTIAK GGKVKIGEIQ GTGTNTLTLP AHFNLTGSIN
     KTGGQALKLN FMNGGSVSGV VGTAANSVGD ITTAGATSFA SSVNAKGTAT LGGTTSFANT
     FTNTGAVTLA KGSITSFAKN VTATSFVANS ATINFSNSLA FNSNITGGGT TLTLGANQVT
     YTGTGSFTDT LTLNTTFDGA AKSGGNILIK SGSTLDLSGV STLALVVTAT NFDMNNISPD
     TKYTVISAET AGGLKPTSKE NVKITINNDN RFVDFTFDAS TLTLFAEDIA ADVIDGDFAP
     GGPLANIPNA ANIKKSLELM EDAPNGSDAR QAFNNFGLMT PLQEADATTH LIQDVVKPSD
     TIAAVNNQVV ASNISSNITA LNARMDKVQS GNKGPVSSGD EDMDAKFGAW ISPFVGNATQ
     KMCNSISGYK SDTTGGTIGF DGFVSDDLAL GLAYTRADTD IKLKNNKTGD KNKVESNIYS
     LYGLYNVPYE NLFVEAIASY SDNKIRSKSR RVIATTLETV GYQTANGKYK SESYTGQLMA
     GYTYMMPENI NLTPLAGLRY STIKDKGYKE TGTTYQNLTV KGKNYNTFDG LLGAKVSSNI
     NVNEIVLTPE LYAMVDYAFK NKVSAIDARL QGMTAPLPTN SFKQSKTSFD VGVGVTAKHK
     MMEYRINYDT NIGSKYFAQQ GSVKVRVNF