ID   OMPA_THEMA              Reviewed;         400 AA.
AC   Q01969;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 126.
DE   RecName: Full=Outer membrane protein alpha;
DE   Flags: Precursor;
GN   Name=omp-alpha; OrderedLocusNames=TM_0477;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=1330536; DOI=10.1002/j.1460-2075.1992.tb05537.x;
RA   Engel A.M., Cejka Z., Lupas A., Lottspeich F., Baumeister W.;
RT   "Isolation and cloning of Omp alpha, a coiled-coil protein spanning the
RT   periplasmic space of the ancestral eubacterium Thermotoga maritima.";
RL   EMBO J. 11:4369-4378(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
CC   -!- FUNCTION: Links the outer membrane to the inner membrane. Long fibrous
CC       protein that could serve to separate the two membranes.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
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DR   EMBL; X68276; CAA48337.1; -; Genomic_DNA.
DR   EMBL; AE000512; AAD35562.1; -; Genomic_DNA.
DR   PIR; E72372; E72372.
DR   RefSeq; NP_228287.1; NC_000853.1.
DR   RefSeq; WP_004081489.1; NZ_CP011107.1.
DR   AlphaFoldDB; Q01969; -.
DR   SMR; Q01969; -.
DR   STRING; 243274.THEMA_02300; -.
DR   PRIDE; Q01969; -.
DR   EnsemblBacteria; AAD35562; AAD35562; TM_0477.
DR   KEGG; tma:TM0477; -.
DR   eggNOG; COG1196; Bacteria.
DR   InParanoid; Q01969; -.
DR   OMA; DHWAYEY; -.
DR   OrthoDB; 554219at2; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR001119; SLH_dom.
DR   Pfam; PF00395; SLH; 1.
DR   PROSITE; PS51272; SLH; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Coiled coil; Direct protein sequencing; Membrane;
KW   Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..20
FT   CHAIN           21..400
FT                   /note="Outer membrane protein alpha"
FT                   /id="PRO_0000032645"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          21..81
FT                   /note="SLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   REPEAT          208..232
FT                   /note="1"
FT   REPEAT          251..275
FT                   /note="2"
FT   REPEAT          326..350
FT                   /note="3"
FT   REGION          208..350
FT                   /note="3 X 25 AA approximate repeat"
FT   COILED          85..379
FT                   /evidence="ECO:0000255"
FT   CONFLICT        179
FT                   /note="V -> A (in Ref. 1; CAA48337)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   400 AA;  45339 MW;  F7C7EB0E80935583 CRC64;
     MKRVLLTVAM LSVFFSAMFA FFPDVPKDHW AYEYVWKLWQ RGIFIGYPDG EFKGDRYITR
     YEAATAVSRL LDFIEQKMLA GASGDLAQVV GNLSDKYMAL EEKVNGLTGI LDTLASQIGT
     TQANLTETER ELLEKIDAVK EEIEMEFDKE ISLNREVVNN IGLKLGNLSR DYERYKENVD
     AKISEVNEKL AALEKDLGNK IADLEGIVNL HEKDIINIYN KISSVNEELN NKIAATEEKL
     SRKDEEISAM VELHEKDIIN LYNKVAALNE DLNKKILDTK AELSAKIESQ EKTLNMVYTK
     LLDTESKLND EISALKEKDA EIQKTVDLHE QDIVNLYGKT SSLEEDLNMK YNETNEKIDQ
     VKAELEDKIE SVKAYNRNLS ILTGAFFGIL GLILIAISGK