ID   OMPB1_NEIMB             Reviewed;         331 AA.
AC   P30690;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 136.
DE   RecName: Full=Major outer membrane protein P.IB;
DE            Short=PIB;
DE            Short=Protein IB;
DE   AltName: Full=Class 3 protein;
DE   AltName: Full=Porin;
DE   Flags: Precursor;
GN   Name=porB; OrderedLocusNames=NMB2039;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=1330818; DOI=10.1016/0378-1097(92)90644-4;
RA   Ward M.J., Lambden P.R., Heckels J.E.;
RT   "Sequence analysis and relationships between meningococcal class 3 serotype
RT   proteins and other porins from pathogenic and non-pathogenic Neisseria
RT   species.";
RL   FEMS Microbiol. Lett. 73:283-289(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17038831; DOI=10.4161/hv.1.2.1651;
RA   Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J.;
RT   "Characterization of the protein content of a meningococcal outer membrane
RT   vesicle vaccine by polyacrylamide gel electrophoresis and mass
RT   spectrometry.";
RL   Hum. Vaccin. 1:80-84(2005).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=NZ98/254 / Serogroup B;
RX   PubMed=16645985; DOI=10.1002/pmic.200500821;
RA   Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.;
RT   "Proteomic analysis of a meningococcal outer membrane vesicle vaccine
RT   prepared from the group B strain NZ98/254.";
RL   Proteomics 6:3400-3413(2006).
CC   -!- FUNCTION: Serves as a slightly cation selective porin.
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein.
CC   -!- MISCELLANEOUS: Present in outer membrane vesicle formulations which are
CC       used as vaccines in human.
CC   -!- SIMILARITY: Belongs to the Gram-negative porin family. {ECO:0000305}.
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DR   EMBL; X65532; CAA46502.1; -; Genomic_DNA.
DR   EMBL; AE002098; AAF42360.1; -; Genomic_DNA.
DR   PIR; S21407; S21407.
DR   RefSeq; NP_275030.1; NC_003112.2.
DR   RefSeq; WP_002244345.1; NC_003112.2.
DR   PDB; 3WI4; X-ray; 3.32 A; A=20-331.
DR   PDB; 3WI5; X-ray; 2.40 A; A=20-331.
DR   PDBsum; 3WI4; -.
DR   PDBsum; 3WI5; -.
DR   AlphaFoldDB; P30690; -.
DR   SMR; P30690; -.
DR   STRING; 122586.NMB2039; -.
DR   PaxDb; P30690; -.
DR   EnsemblBacteria; AAF42360; AAF42360; NMB2039.
DR   KEGG; nme:NMB2039; -.
DR   PATRIC; fig|122586.8.peg.2608; -.
DR   HOGENOM; CLU_038238_4_0_4; -.
DR   OMA; YKADAMS; -.
DR   Reactome; R-HSA-1236974; ER-Phagosome pathway.
DR   Reactome; R-HSA-166058; MyD88:MAL(TIRAP) cascade initiated on plasma membrane.
DR   Reactome; R-HSA-168179; Toll Like Receptor TLR1:TLR2 Cascade.
DR   Reactome; R-HSA-5602498; MyD88 deficiency (TLR2/4).
DR   Reactome; R-HSA-5603041; IRAK4 deficiency (TLR2/4).
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0034220; P:ion transmembrane transport; IEA:InterPro.
DR   CDD; cd00342; gram_neg_porins; 1.
DR   Gene3D; 2.40.160.10; -; 1.
DR   InterPro; IPR033900; Gram_neg_porin_domain.
DR   InterPro; IPR023614; Porin_dom_sf.
DR   InterPro; IPR001702; Porin_Gram-ve.
DR   InterPro; IPR013793; Porin_Gram-ve_CS.
DR   InterPro; IPR002299; Porin_Neis.
DR   Pfam; PF00267; Porin_1; 1.
DR   PRINTS; PR00182; ECOLNEIPORIN.
DR   PRINTS; PR00184; NEISSPPORIN.
DR   PROSITE; PS00576; GRAM_NEG_PORIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Ion transport; Membrane; Porin;
KW   Reference proteome; Signal; Transmembrane; Transmembrane beta strand;
KW   Transport.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000250"
FT   CHAIN           20..331
FT                   /note="Major outer membrane protein P.IB"
FT                   /id="PRO_0000025281"
FT   STRAND          21..39
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          41..55
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          59..69
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          72..81
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          94..102
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          105..113
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   HELIX           116..118
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   TURN            130..134
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   TURN            136..140
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          143..150
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          157..164
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   HELIX           166..169
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          176..185
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          188..200
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          203..205
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          208..222
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          225..240
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          245..256
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          262..271
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          277..279
FT                   /evidence="ECO:0007829|PDB:3WI4"
FT   STRAND          285..310
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          314..316
FT                   /evidence="ECO:0007829|PDB:3WI5"
FT   STRAND          318..331
FT                   /evidence="ECO:0007829|PDB:3WI5"
SQ   SEQUENCE   331 AA;  35682 MW;  D6945BC81B04B6AF CRC64;
     MKKSLIALTL AALPVAAMAD VTLYGTIKAG VETSRSVFHQ NGQVTEVTTA TGIVDLGSKI
     GFKGQEDLGN GLKAIWQVEQ KASIAGTDSG WGNRQSFIGL KGGFGKLRVG RLNSVLKDTG
     DINPWDSKSD YLGVNKIAEP EARLISVRYD SPEFAGLSGS VQYALNDNAG RHNSESYHAG
     FNYKNGGFFV QYGGAYKRHH QVQEGLNIEK YQIHRLVSGY DNDALYASVA VQQQDAKLTD
     ASNSHNSQTE VAATLAYRFG NVTPRVSYAH GFKGLVDDAD IGNEYDQVVV GAEYDFSKRT
     SALVSAGWLQ EGKGENKFVA TAGGVGLRHK F