OMPB_RICCN
ID OMPB_RICCN Reviewed; 1655 AA.
AC Q9KKA3; Q9KK98; Q9XC45;
DT 11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2001, sequence version 2.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Outer membrane protein B;
DE AltName: Full=168 kDa surface-layer protein;
DE AltName: Full=Cell surface antigen 5;
DE Short=Sca5;
DE AltName: Full=Surface protein antigen;
DE AltName: Full=rOmp B;
DE Short=rOmpB;
DE Contains:
DE RecName: Full=120 kDa surface-exposed protein;
DE AltName: Full=120 kDa outer membrane protein OmpB;
DE AltName: Full=Surface protein antigen;
DE AltName: Full=p120;
DE Contains:
DE RecName: Full=32 kDa beta peptide;
DE Flags: Precursor;
GN Name=ompB; OrderedLocusNames=RC1085;
OS Rickettsia conorii (strain ATCC VR-613 / Malish 7).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX NCBI_TaxID=272944;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC VR-613 / Malish 7;
RX PubMed=11557893; DOI=10.1126/science.1061471;
RA Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V.,
RA Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.;
RT "Mechanisms of evolution in Rickettsia conorii and R. prowazekii.";
RL Science 293:2093-2098(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-1649.
RC STRAIN=ATCC VR-613 / Malish 7, and Indian tick typhus;
RX PubMed=10939649; DOI=10.1099/00207713-50-4-1449;
RA Roux V., Raoult D.;
RT "Phylogenetic analysis of members of the genus Rickettsia using the gene
RT encoding the outer-membrane protein rOmpB (ompB).";
RL Int. J. Syst. Evol. Microbiol. 50:1449-1455(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 353-1655.
RC STRAIN=ATCC VR-613 / Malish 7;
RX PubMed=11034486; DOI=10.1099/00207713-50-5-1775;
RA Stenos J., Walker D.H.;
RT "The rickettsial outer-membrane protein A and B genes of Rickettsia
RT australis, the most divergent rickettsia of the spotted fever group.";
RL Int. J. Syst. Evol. Microbiol. 50:1775-1779(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND ADHESION TO BIOTINYLATED
RP EUKARYOTIC CELLS.
RC STRAIN=ATCC VR-613 / Malish 7;
RX PubMed=16574381; DOI=10.1016/j.resmic.2006.02.002;
RA Renesto P., Samson L., Ogata H., Azza S., Fourquet P., Gorvel J.-P.,
RA Heinzen R.A., Raoult D.;
RT "Identification of two putative rickettsial adhesins by proteomic
RT analysis.";
RL Res. Microbiol. 157:605-612(2006).
CC -!- FUNCTION: The 120 kDa surface-exposed protein is a major structural
CC protein which may play a role as a rickettsial virulence factor and/or
CC immunogen during infection. {ECO:0000250}.
CC -!- FUNCTION: The 32 kDa beta peptide may serve as a membrane anchor (By
CC similarity). It has been shown to adhere to biotinylated Vero cell
CC proteins. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Outer membrane protein B]: Periplasm
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [120 kDa surface-exposed protein]: Secreted
CC {ECO:0000250}. Cell surface {ECO:0000250}. Note=Surface exposed. This
CC bacterium is covered by a S-layer with hexagonal symmetry (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [32 kDa beta peptide]: Cell outer membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=The
CC cleaved C-terminal fragment (autotransporter domain) is localized in
CC the outer membrane.
CC -!- SIMILARITY: Belongs to the rickettsiae OmpA/OmpB family. {ECO:0000305}.
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DR EMBL; AE006914; AAL03623.1; -; Genomic_DNA.
DR EMBL; AF123721; AAF34124.1; -; Genomic_DNA.
DR EMBL; AF123726; AAF34129.1; -; Genomic_DNA.
DR EMBL; AF149110; AAD39533.1; -; Genomic_DNA.
DR PIR; E97835; E97835.
DR RefSeq; WP_010977663.1; NC_003103.1.
DR AlphaFoldDB; Q9KKA3; -.
DR SMR; Q9KKA3; -.
DR EnsemblBacteria; AAL03623; AAL03623; RC1085.
DR KEGG; rco:RC1085; -.
DR PATRIC; fig|272944.4.peg.1245; -.
DR HOGENOM; CLU_000413_0_0_5; -.
DR OMA; YPEVHAF; -.
DR Proteomes; UP000000816; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR Gene3D; 2.40.128.130; -; 1.
DR InterPro; IPR005546; Autotransporte_beta.
DR InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR InterPro; IPR006315; OM_autotransptr_brl.
DR InterPro; IPR022095; rOmpB.
DR Pfam; PF03797; Autotransporter; 1.
DR Pfam; PF12334; rOmpB; 1.
DR SMART; SM00869; Autotransporter; 1.
DR SUPFAM; SSF103515; SSF103515; 1.
DR TIGRFAMs; TIGR01414; autotrans_barl; 1.
DR PROSITE; PS51208; AUTOTRANSPORTER; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Membrane; Periplasm; Secreted; Transmembrane;
KW Transmembrane beta strand; Virulence.
FT CHAIN 1..1655
FT /note="Outer membrane protein B"
FT /id="PRO_0000387579"
FT CHAIN 1..1334
FT /note="120 kDa surface-exposed protein"
FT /id="PRO_0000032648"
FT PROPEP 1335..1362
FT /evidence="ECO:0000255"
FT /id="PRO_0000032649"
FT CHAIN 1363..1655
FT /note="32 kDa beta peptide"
FT /id="PRO_0000032650"
FT DOMAIN 1367..1655
FT /note="Autotransporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT VARIANT 61
FT /note="P -> A (in strain: Indian tick typhus)"
FT VARIANT 75
FT /note="G -> S (in strain: Indian tick typhus)"
FT VARIANT 78
FT /note="K -> N (in strain: Indian tick typhus)"
FT VARIANT 251
FT /note="V -> A (in strain: Indian tick typhus)"
FT VARIANT 413
FT /note="N -> D (in strain: Indian tick typhus)"
FT VARIANT 959
FT /note="I -> V (in strain: Indian tick typhus)"
FT VARIANT 988
FT /note="A -> T (in strain: Indian tick typhus)"
FT VARIANT 1139
FT /note="R -> L (in strain: Indian tick typhus)"
FT CONFLICT 353..354
FT /note="KD -> GH (in Ref. 3; AAD39533)"
FT /evidence="ECO:0000305"
FT CONFLICT 776
FT /note="F -> S (in Ref. 3; AAD39533)"
FT /evidence="ECO:0000305"
FT CONFLICT 1159
FT /note="E -> D (in Ref. 3; AAD39533)"
FT /evidence="ECO:0000305"
FT CONFLICT 1177
FT /note="G -> S (in Ref. 3; AAD39533)"
FT /evidence="ECO:0000305"
FT CONFLICT 1492
FT /note="H -> R (in Ref. 3; AAD39533)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1655 AA; 168343 MW; E49E19377D5FCE37 CRC64;
MAQKPNFLKK LISAGLVTAS TATIVASFAG SAMGAAIQQN RTTNAVATTV DGVGFDQTAV
PANVAVPLNA VITAGVNKGI TLNTPAGSFN GLFLNTANNL DVTVREDTTL GFITNVVNNA
NHFNLMLNAG KTLTITGQGI TNVQAAATKN ANNVVAQVNN GAAIDNNDLQ GVGRIDCGAA
ASTLVFNLAN PTTQKAPLIL GDNAVIVNGA NGTLNVTNGF IKVSSKSFAT VNVINIGDGQ
GIMFNTDADN VNTLNLQANG ATITFNGTDG TGRLVLLSKN AAATDFNVTG SLGGNLKGII
EFNTVAVNGQ LKANAGANAA VIGTNNGAGR AAGFVVSVDN GKVATIDGQV YAKDMVIQSA
NAVGQVNFRH IVDVGTDGTT AFKTAASKVA ITQNSNFGTT DFGNLAAQII VPNTMTLNGN
FTGDASNPGN TAGVITFDAN GTLASASADA NVAVTNNITA IEASGAGVVQ LSGTHAAELR
LGNAGSVFKL ADGTVINGKV NQTALVGGAL AAGTITLDGS ATITGDIGNA GGAAALQGIT
LANDATKTLT LGGANIIGAN GGTINFQANG GTIKLTSTQN NIVVDFDLAI ATDQTGVVDA
SSLTNAQTLT INGKIGTVGA NNKTLGQFNI GSSKTVLSDG DVAINELVIG NNGAVQFAHN
TYLITRTTNA AGQGKIIFNP VVNNNTTLAT GTNLGSATNP LAEINFGSKG AANVDTVLNV
GKGVNLYATN ITTTDANVGS FIFNAGGTNI VSGTVGGQQG NKFNTVALDN GTTVKFLGNA
TFNGNTTIAA NSTLQIGGNY TADFVASADG TGIVEFVNTG PITVTLNKQA APVNALKQIT
VSGPGNVVIN EIGNAGNYHG AVTDTIAFEN SSLGAVVFLP RGIPFNDAGN RIPLTIKSTV
GNKTATGFDV PSVIVLGVDS VIADGQVIGD QNNIVGLGLG SDNDIIVNAT TLYAGIGTIN
NNQGTVTLSG GIPNTPGTVY GLGTGIGASK FKQVTFTTDY NNLGNIIATN ATINDGVTVT
TGGIAGIGFD GKITLGSVNG NGNVRFVDGI LSHSTSMIGT TKANNGTVTY LGNAFVGNIG
DSDTPVASVR FTGSDGGAGL QGNIYSQVID FGTYNLGISN SNVILGGGTT AINGKINLRT
NTLTFASGTS TWGNNTSIET TLTLANGNIG NIVILEGAQV NATTTGTTTI KVQDNANANF
SGTQTYTLIQ GGARFNGTLG GPNFVVTGSN RFVNYGLIRA ANQDYVITRT NNAENVVTND
IANSSFGGAP GVGQNVTTFV NATNTAAYNN LLLAKNSANS ANFVGAIVTD TSAAITNAQL
DVAKDIQAQL GNRLGALRYL GTPETAEMAG PEAGAIPAAV AAGDEAVDNV AYGIWAKPFY
TDAHQSKKGG LAGYKAKTTG VVIGLDTLAN DNLMIGAAIG ITKTDIKHQD YKKGDKTDVN
GFSFSLYGAQ QLVKNFFAQG SAIFSLNQVK NKSQRYFFDA NGNMSKQIAA GHYDNMTFGG
NLTVGYDYNA MQGVLVTPMA GLSYLKSSDE NYKETGTTVA NKQVNSKFSD RTDLIVGAKV
AGSTMNITDL AVYPEVHAFV VHKVTGRLSK TQSVLDGQVT PCISQPDRTA KTSYNLGLSA
SIRSDAKMEY GIGYDAQISS KYTAHQGTLK VRVNF
