ID   OMPB_RICJY              Reviewed;        1656 AA.
AC   O06653; G4KLB3;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Outer membrane protein B;
DE   AltName: Full=168 kDa surface-layer protein;
DE   AltName: Full=Cell surface antigen 5;
DE            Short=Sca5;
DE   AltName: Full=Surface protein antigen;
DE   AltName: Full=rOmp B;
DE            Short=rOmpB;
DE   Contains:
DE     RecName: Full=120 kDa surface-exposed protein;
DE     AltName: Full=120 kDa outer membrane protein OmpB;
DE     AltName: Full=Surface protein antigen;
DE     AltName: Full=p120;
DE   Contains:
DE     RecName: Full=32 kDa beta peptide;
DE   Flags: Precursor;
GN   Name=ompB; OrderedLocusNames=RJP_0809;
OS   Rickettsia japonica (strain ATCC VR-1363 / YH).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=652620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC VR-1363 / YH;
RA   Uchiyama T.;
RT   "Sequencing of the gene encoding the protein rOmp B of Rickettsia
RT   japonica.";
RL   Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1363 / YH;
RX   PubMed=24039725; DOI=10.1371/journal.pone.0071861;
RA   Matsutani M., Ogawa M., Takaoka N., Hanaoka N., Toh H., Yamashita A.,
RA   Oshima K., Hirakawa H., Kuhara S., Suzuki H., Hattori M., Kishimoto T.,
RA   Ando S., Azuma Y., Shirai M.;
RT   "Complete genomic DNA sequence of the East Asian spotted fever disease
RT   agent Rickettsia japonica.";
RL   PLoS ONE 8:E71861-E71861(2013).
CC   -!- FUNCTION: The 120 kDa surface-exposed protein is a major structural
CC       protein which may play a role as a rickettsial virulence factor and/or
CC       immunogen during infection. {ECO:0000250}.
CC   -!- FUNCTION: The 32 kDa beta peptide may serve as a membrane anchor.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Outer membrane protein B]: Periplasm
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [120 kDa surface-exposed protein]: Secreted. Cell
CC       surface. Note=Surface exposed. This bacterium is covered by a S-layer
CC       with hexagonal symmetry (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [32 kDa beta peptide]: Cell outer membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=The
CC       cleaved C-terminal fragment (autotransporter domain) is localized in
CC       the outer membrane.
CC   -!- SIMILARITY: Belongs to the rickettsiae OmpA/OmpB family. {ECO:0000305}.
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DR   EMBL; AB003681; BAA20138.1; -; Genomic_DNA.
DR   EMBL; AP011533; BAK97005.1; -; Genomic_DNA.
DR   PIR; JC1340; JC1340.
DR   RefSeq; WP_014121034.1; NZ_AMRT02000001.1.
DR   AlphaFoldDB; O06653; -.
DR   SMR; O06653; -.
DR   EnsemblBacteria; BAK97005; BAK97005; RJP_0809.
DR   KEGG; rja:RJP_0809; -.
DR   HOGENOM; CLU_000413_0_0_5; -.
DR   OMA; YPEVHAF; -.
DR   Proteomes; UP000002659; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.128.130; -; 1.
DR   InterPro; IPR005546; Autotransporte_beta.
DR   InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR   InterPro; IPR006315; OM_autotransptr_brl.
DR   InterPro; IPR022095; rOmpB.
DR   Pfam; PF03797; Autotransporter; 1.
DR   Pfam; PF12334; rOmpB; 1.
DR   SMART; SM00869; Autotransporter; 1.
DR   SUPFAM; SSF103515; SSF103515; 1.
DR   TIGRFAMs; TIGR01414; autotrans_barl; 1.
DR   PROSITE; PS51208; AUTOTRANSPORTER; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Periplasm; Secreted; Transmembrane;
KW   Transmembrane beta strand; Virulence.
FT   CHAIN           1..1656
FT                   /note="Outer membrane protein B"
FT                   /id="PRO_0000387580"
FT   CHAIN           1..1338
FT                   /note="120 kDa surface-exposed protein"
FT                   /id="PRO_0000032651"
FT   PROPEP          1339..1363
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000032652"
FT   CHAIN           1364..1656
FT                   /note="32 kDa beta peptide"
FT                   /id="PRO_0000032653"
FT   DOMAIN          1368..1656
FT                   /note="Autotransporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
SQ   SEQUENCE   1656 AA;  168098 MW;  3132A69C9DD5999F CRC64;
     MAQKPNFLKK LISAGLVTAS TATIVASFAG SAMGAAIQQN RTTNGVATTV DGVGFDQTVA
     LANVAVAPNA VITANANNGI NLNTPAGSFN GLFLSNANNL AVTVSEDTTL GFINNAANNA
     NRFNLTLDAG KTLTITGQGI TNVQSAATHN AQNIVAKFNG GAAIANNDLS GLGTIDFGAA
     ASTLVFDLAN PTTQKAPLIL ADNALIVNGA NGTLNVTNGF IQVSDKSFAT VKAINIGDGQ
     GFMFNTNATN ANALNLQAGG TTINFNGTDG TGRLVLLSKN GAATDFNVTG SLGGNLKGII
     ELNTVAINGQ LIANAGPANA VIGTNNGAGR AAGFVVSVDN GKAATIDGQV YAKDMVIQSA
     NANGQVNFRH IVDVGIDGTT AFKTAASIVA ITQNSNFGTT DFGNLAAQVT VPDTMTLTGN
     FTGDANNPGN TAGVITFAAN GTLASASADA NVAVTNNITA IEASGVGVVQ LSGTHTAELR
     LGNAGSVFKL ADGTVINGKV NQTVLVGGVL AAGAITLDGS ATITGDIGNG GGGAALQSIT
     LANDATKTLT LGGANIISAN GGTINFQANG GTIKLTSTQN NIVVDCDLAI ATDQTGVVDA
     SSLTNAQTLT ISGTIGIIGA NNTTLGQFNI GSSKTTLNGG NVAINELVIG NNGSVQFAHN
     TYLITRTTNA AGQGKIIFNP VVNNNTTLAA GTNLGSAANP LAEINFGSKG ARADTVLNVG
     EGVNLYATNI TTTDANVGSF VFNAGGKNIV SGTVGGQQGN KFNTVALDNG TTVKFLGNAT
     FNGNTTIAAN STLQISGNYT ADFIASADGT GIVEFVNTGP INVTLNKQAV PVNALKQITV
     SGPGNVVVNE IGNAGNYHGA MTDTIAFENS SLGAVLFLPS GIPFNDAGNT IPLTIKSTVG
     NETAEGFSVP SVIVSGVDSV IADGQVIGDQ NNIVGLGLGS DNGIIVNATT LYAGIGTINN
     NQGTVTLSGG VPNTPGTVYG LGTGIGASKF KQVTFTTDYN NLGNIIATNT TINDGVTVTT
     GGIAAGGIAG TDFDGKITLG SVNGNANVRF ADGIFSNSTS MIVTTKANNG TVTYLGNAFV
     GNIGDSDTPV ASVRFTGSNN GAGLKGNIYS QVIDFGTYNL GIVNSNVILG GSTTAINGKI
     DLLTNTLTFA GGTSTWGNNT SIETTLTLAN GNIGHIVIAE GAQVNATTTG TTTINVQDNA
     NANFSGTQTY TLIQGGARFN GTLGGPNFTV TGSNRFVNYG LIRAANQDYV ITRTNNAENI
     VTNDITNSPF GGAPGVGQNV TTFVNATNTA AYNNLLLAKN SADSANFVGT IVTDTSAAIT
     NAQLDVAKDI QAQLGNRLGA LRYLGTPEMV GSEAGAIPAA VAAGDEAVDN VAYGIWAKPF
     YTDAHQSKKG GLAGYKAKTT GIVIGLDTLA NNNLMIGAAI GITKTDIKHQ DYKKGDKTDV
     NGFSFSLYGA QQFVENFFAQ GSAIFSLNQV KNKSQRYFFD ANGNMSKQIA AGNYDNMTFG
     GNLTVGYDYN AMQGVLVTPM AGLSYLKSSD ENYKETGTTV ANKQVNSKFS DRTDLIVGAK
     VAGGTMNITD FAVYPEVHAF VVHKVTGRLS KTQSVLDGQV TPCISQPDRT AKTSYNLGLS
     ASIRSDAKME YGIGYDAQIA SKYTAHQGTL KVRVNF