OMPB_RICRI
ID OMPB_RICRI Reviewed; 1654 AA.
AC Q53047; P14914;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Outer membrane protein B;
DE AltName: Full=168 kDa surface-layer protein;
DE AltName: Full=Cell surface antigen 5;
DE Short=Sca5;
DE AltName: Full=Surface protein antigen;
DE AltName: Full=rOmp B;
DE Short=rOmpB;
DE Contains:
DE RecName: Full=120 kDa surface-exposed protein;
DE AltName: Full=120 kDa outer membrane protein OmpB;
DE AltName: Full=Surface protein antigen;
DE AltName: Full=p120;
DE Contains:
DE RecName: Full=32 kDa beta peptide;
DE Flags: Precursor;
GN Name=ompB;
OS Rickettsia rickettsii.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX NCBI_TaxID=783;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE (N-TERMINAL
RP OF THE 32 KDA BETA PEPTIDE).
RC STRAIN=R;
RX PubMed=1724278; DOI=10.1111/j.1365-2958.1991.tb02082.x;
RA Gilmore R.D. Jr., Cieplak W. Jr., Policastro P.F., Hackstadt T.;
RT "The 120 kilodalton outer membrane protein (rOmp B) of Rickettsia
RT rickettsii is encoded by an unusually long open reading frame: evidence for
RT protein processing from a large precursor.";
RL Mol. Microbiol. 5:2361-2370(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-1654.
RC STRAIN=R;
RX PubMed=2515418; DOI=10.1111/j.1365-2958.1989.tb00143.x;
RA Gilmore R.D. Jr., Joste N., McDonald G.A.;
RT "Cloning, expression and sequence analysis of the gene encoding the 120 kD
RT surface-exposed protein of Rickettsia rickettsii.";
RL Mol. Microbiol. 3:1579-1586(1989).
RN [3]
RP ERRATUM OF PUBMED:2515418, AND SEQUENCE REVISION TO 1348-1358.
RX PubMed=1809847;
RA Gilmore R.D. Jr., Joste N., McDonald G.A.;
RL Mol. Microbiol. 5:3089-3089(1991).
RN [4]
RP PROTEIN SEQUENCE OF 1362-1380, AND CLEAVAGE SITE.
RC STRAIN=R;
RX PubMed=1729180; DOI=10.1128/iai.60.1.159-165.1992;
RA Hackstadt T., Messer R., Cieplak W. Jr., Peacock M.G.;
RT "Evidence for proteolytic cleavage of the 120-kilodalton outer membrane
RT protein of rickettsiae: identification of an avirulent mutant deficient in
RT processing.";
RL Infect. Immun. 60:159-165(1992).
CC -!- FUNCTION: The 120 kDa surface-exposed protein is a major structural
CC protein which may play a role as a rickettsial virulence factor and/or
CC immunogen during infection.
CC -!- FUNCTION: The 32 kDa beta peptide may serve as a membrane anchor.
CC -!- SUBCELLULAR LOCATION: [Outer membrane protein B]: Periplasm
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [120 kDa surface-exposed protein]: Secreted. Cell
CC surface. Note=Surface exposed. This bacterium is covered by a S-layer
CC with hexagonal symmetry.
CC -!- SUBCELLULAR LOCATION: [32 kDa beta peptide]: Cell outer membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=The
CC cleaved C-terminal fragment (autotransporter domain) is localized in
CC the outer membrane.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the rickettsiae OmpA/OmpB family. {ECO:0000305}.
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DR EMBL; X16353; CAA34403.1; -; Genomic_DNA.
DR EMBL; X16353; CAA34402.1; -; Genomic_DNA.
DR PIR; S07575; S07575.
DR PIR; S18227; S18227.
DR AlphaFoldDB; Q53047; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR Gene3D; 2.40.128.130; -; 1.
DR InterPro; IPR005546; Autotransporte_beta.
DR InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR InterPro; IPR006315; OM_autotransptr_brl.
DR InterPro; IPR022095; rOmpB.
DR Pfam; PF03797; Autotransporter; 1.
DR Pfam; PF12334; rOmpB; 1.
DR SMART; SM00869; Autotransporter; 1.
DR SUPFAM; SSF103515; SSF103515; 1.
DR TIGRFAMs; TIGR01414; autotrans_barl; 1.
DR PROSITE; PS51208; AUTOTRANSPORTER; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Direct protein sequencing; Membrane; Periplasm;
KW Secreted; Transmembrane; Transmembrane beta strand; Virulence.
FT CHAIN 1..1654
FT /note="Outer membrane protein B"
FT /id="PRO_0000387582"
FT CHAIN 1..1333
FT /note="120 kDa surface-exposed protein"
FT /id="PRO_0000032657"
FT PROPEP 1334..1361
FT /evidence="ECO:0000255"
FT /id="PRO_0000032658"
FT CHAIN 1362..1654
FT /note="32 kDa beta peptide"
FT /id="PRO_0000032659"
FT DOMAIN 1366..1654
FT /note="Autotransporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
SQ SEQUENCE 1654 AA; 168184 MW; D7AB70FB7087F618 CRC64;
MAQKPNFLKK LISAGLVTAS TATIVASFAG SAMGAAIQQN RTTNGAATTV DGAGFDQTAA
PANVGVALNA VITANANNGI NFNTPAGSFN GLLLNTANNL AVTVSEDTTL GFITNVVHNA
HSFNLTLNAG KTLTITGQGV TNAQAAATKN AQNVVVQFNN GAAIDNNDLK GVGRIDFGAP
ASTLVFNLAN PTTQKAPLIL GDNAVIANGV NGTLNVTNGF IQVSNKSFAT VKAINIADGQ
GIIFNTDANN ANTLNLQAGG TTINFTGTDG TGRLVLLSKH AAATNFNITG SLGGNLKGVI
EFNTVAVDGQ LTANAGAANA VIGTNNGAGR AAGFVVSVDN GKVATIDGQV YAKDMVIQSA
NATGQVNFRH IVDVGADGTT AFKTAASKVT ITQDSNFGNT DFGNLAAQIK VPNAITLTGN
FTGDASNPGN TAGVITFDAN GTLESASADA NVAVTNNITA IEASGAGVVQ LSGTHAAELR
LGNAGSIFKL ADGTVINGKV NQTALVGGAL AAGTITLDGS ATITGDIGNA GGAAALQRIT
LANDAKKTLT LGGANIIGAG GGTIDLQANG GTIKLTSTQN NIVVDFDLAI ATDQTGVVDA
SSLTNAQTLT INGKIGTIGA NNKTLGQFNI GSSKTVLSNG NVAINELVIG NDGAVQFAHD
TYLITRTTNA AGQGKIIFNP VVNNGTTLAA GTNLGSATNP LAEINFGSKG VNVDTVLNVG
EGVNLYATNI TTTDANVGSF VFNAGGTNIV SGTVGGQQGN KFNTVALENG TTVKFLGNAT
FNGNTTIAAN STLQIGGNYT ADCVASADGT GIVEFVNTGP ITVTLNKQAA PVNALKQITV
SGPGNVVINE IGNAGNHHGA VTDTIAFENS SLGAVVFLPR GIPFNDAGNT MPLTIKSTVG
NKTAKGFDVP SVVVLGVDSV IADGQVIGDQ NNIVGLGLGS DNGIIVNATT LYAGISTLNN
NQGTVTLSGG VPNTPGTVYG LGTGIGASKF KQVTFTTDYN NLGNIIATNA TINDGVTVTT
GGIAGIGFDG KITLGSVNGN GNVRFADGIL SNSTSMIGTT KANNGTVTYL GNAFVGNIGD
SDTPVASVRF TGSDSGAGLQ GNIYSQVIDF GTYNLGIVNS NIILGGGTTA INGKIDLVTN
TLTFASGTST WGNNTSIETT LTLANGNIGH IVILEGAQVN TTTTGTTTIK VQDNANANFS
GTQTYTLIQG GARFNGTLGS PNFAVTGSNR FVNYSLIRAA NQDYVITRTN NAENVVTNDI
ANSPFGGAPG VDQNVTTFVN ATNTAAYNNL LLAKNSANSA NFVGAIVTDT SAAITNVQLD
LAKDIQAQLG NRLGALRYLG TPETAEMAGP EAGAISAAVA AGDEAIDNVA YGIWAKPFYT
DAHQSKKGGL AGYKAKTTGV VIGLDTLAND NLMIGAAIGI TKTDIKHQDY KKGDKTDVNG
FSFSLYGAQQ LVKNFFAQGS AIFSLNQVKN KSQRYFFDAN GNMSKQIAAG HYDNMTFGGN
LTVGYDYNAM QGVLVTPMAG LSYLKSSDEN YKETGTTVAN KQVNSKFSDR TDLIVGAKVA
GSTMNITDLA VYPEVHAFVV HKVTGRLSKT QSVLDGQVTP CINQPDRTTK TSYNLGLSAS
IRSDAKMEYG IGYDAQISSK YTAHQGTLKV RVNF
