OMPR_SALTI
ID OMPR_SALTI Reviewed; 239 AA.
AC P0AA20; O31133; P03025; P08981; P41405;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=DNA-binding dual transcriptional regulator OmpR {ECO:0000305};
DE AltName: Full=Transcriptional regulatory protein OmpR {ECO:0000303|PubMed:8063417};
GN Name=ompR {ECO:0000303|PubMed:8063417};
GN Synonyms=ompB {ECO:0000303|PubMed:8063417};
GN OrderedLocusNames=STY4294, t4004;
OS Salmonella typhi.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=90370;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN VIRULENCE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=ATCC 700931 / Ty2 / CVD908;
RX PubMed=8063417; DOI=10.1128/iai.62.9.3984-3993.1994;
RA Pickard D.J., Li J., Roberts M.R., Maskell D., Hone D., Levine M.,
RA Dougan G., Chatfield S.;
RT "Characterization of defined ompR mutants of Salmonella typhi: ompR is
RT involved in the regulation of Vi polysaccharide expression.";
RL Infect. Immun. 62:3984-3993(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=IMSS-1;
RA Martinez-Flores I., Bustamante V., Puente J.L., Calva E.;
RT "Cloning and characterization of the Salmonella typhi ompR and envZ
RT genes.";
RL Asia Pac. J. Mol. Biol. Biotechnol. 3:135-144(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CT18;
RX PubMed=11677608; DOI=10.1038/35101607;
RA Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A.,
RA Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T.,
RA Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A.,
RA Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A.,
RA Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S.,
RA Barrell B.G.;
RT "Complete genome sequence of a multiple drug resistant Salmonella enterica
RT serovar Typhi CT18.";
RL Nature 413:848-852(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700931 / Ty2;
RX PubMed=12644504; DOI=10.1128/jb.185.7.2330-2337.2003;
RA Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V.,
RA Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and
RT CT18.";
RL J. Bacteriol. 185:2330-2337(2003).
CC -!- FUNCTION: Member of the two-component regulatory system EnvZ/OmpR
CC involved in regulating expression of the outer membrane porins OmpC and
CC OmpF as well as other genes. Unlike E.coli or S.typhimurium both porins
CC are expressed constitutively. Involved in regulation of the
CC biosynthesis of Vi polysaccharide, a capsular antigen thought to be
CC involved in the virulence of S.typhi. Vi antigen is synthesized at low
CC NaCl concentrations (under 0.4 M) (PubMed:8063417). Binds to the
CC promoter of both ompC and ompF (By similarity).
CC {ECO:0000250|UniProtKB:P0AA16, ECO:0000269|PubMed:8063417}.
CC -!- SUBUNIT: Monomer and multimer. {ECO:0000250|UniProtKB:P0AA16}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0AA16}.
CC -!- PTM: Phosphorylated by EnvZ; this stimulates its DNA-binding ability.
CC Asp-55 is the primary phosphate acceptor site.
CC {ECO:0000250|UniProtKB:P0AA16}.
CC -!- DISRUPTION PHENOTYPE: Loss of expression of OmpC and OmpF under low and
CC high osmolarity. No longer agglutinates with Vi antiserum, agglutinates
CC with 09 antiserum without boiling, indicating the Vi polysaccharide is
CC not masking the lipopolysaccharide antigen.
CC {ECO:0000269|PubMed:8063417}.
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DR EMBL; X78270; CAA55076.1; -; Genomic_DNA.
DR EMBL; X77305; CAA54510.1; -; Genomic_DNA.
DR EMBL; AL513382; CAD08112.1; -; Genomic_DNA.
DR EMBL; AE014613; AAO71474.1; -; Genomic_DNA.
DR PIR; S41887; S41887.
DR RefSeq; NP_458402.1; NC_003198.1.
DR RefSeq; WP_001157751.1; NZ_WSUR01000001.1.
DR AlphaFoldDB; P0AA20; -.
DR SMR; P0AA20; -.
DR STRING; 220341.16505091; -.
DR EnsemblBacteria; AAO71474; AAO71474; t4004.
DR GeneID; 8912757; -.
DR KEGG; stt:t4004; -.
DR KEGG; sty:STY4294; -.
DR PATRIC; fig|220341.7.peg.4388; -.
DR eggNOG; COG0745; Bacteria.
DR HOGENOM; CLU_000445_30_4_6; -.
DR OMA; NIHGEGF; -.
DR Proteomes; UP000000541; Chromosome.
DR Proteomes; UP000002670; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Repressor;
KW Stress response; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..239
FT /note="DNA-binding dual transcriptional regulator OmpR"
FT /id="PRO_0000081179"
FT DOMAIN 6..120
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 135..234
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 55
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000250|UniProtKB:P0AA16,
FT ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 118
FT /note="A -> P (in Ref. 1; CAA55076)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 239 AA; 27354 MW; 823CA7720E9A1D2A CRC64;
MQENYKILVV DDDMRLRALL ERYLTEQGFQ VRSVANAEQM DRLLTRESFH LMVLDLMLPG
EDGLSICRRL RSQSNPMPII MVTAKGEEVD RIVGLEIGAD DYIPKPFNPR ELLARIRAVL
RRQANELPGA PSQEEAVIAF GKFKLNLGTR EMFREDEPMP LTSGEFAVLK ALVSHPREPL
SRDKLMNLAR GREYSAMERS IDVQISRLRR MVEEDPAHPR YIQTVWGLGY VFVPDGSKA
